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Article

Anti-Metastatic Effects of Plant Sap-Derived Extracellular Vesicles in a 3D Microfluidic Cancer Metastasis Model

1
Department of Integrative Biosciences, University of Brain Education, Cheonan 31228, Korea
2
Department of Bio and Brain engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea
3
Electron Microscopy Research Center, Korea Basic Science Institute, Cheongju 28119, Korea
4
School of Integrative Engineering, Chung-Ang University, Seoul 06911, Korea
*
Author to whom correspondence should be addressed.
J. Funct. Biomater. 2020, 11(3), 49; https://doi.org/10.3390/jfb11030049
Received: 24 April 2020 / Revised: 7 June 2020 / Accepted: 23 June 2020 / Published: 8 July 2020
(This article belongs to the Special Issue Application of Biomechanical Model on Tissue Engineering)
Natural medicinal plants have attracted considerable research attention for their potential as effective drugs. The roots, leaves and stems of the plant, Dendropanax morbifera, which is endemic to southern regions of Asia, have long been used as a folk medicine to treat variety of diseases. However, the sap of this plant has not been widely studied and its bioactive properties have yet to be clearly elucidated. Here, we isolated extracellular vesicles from D. morbifera sap with the goal of improving the intracellular delivery efficiency and clinical effectiveness of bioactive compounds in D. morbifera sap. We further investigated the anti-metastatic effects of D. morbifera sap-derived extracellular vesicles (DMS-EVs) using a cancer metastasis model based on 3D microfluidic system that closely mimics the in vivo tumor environment. We found that DMS-EVs exerted a concentration-dependent suppressive effect on cancer-associated fibroblasts (CAFs), which are important mediators of cancer metastasis. DMS-EVs also altered expression level of genes, especially growth factor and extracellular matrix (ECM)-related genes, including integrin and collagen. Our findings suggest that DMS-EVs can act as anti-CAF agents to reduce CAFs in the tumor microenvironment. They further indicate the utility of our 3D microfluidic model for various drug-screening assays as a potential alternative to animal testing for use in validating therapeutic effects on cancer metastasis. View Full-Text
Keywords: cancer-associated fibroblasts; plant sap; extracellular vesicles; anti-metastatic effects; 3D microfluidics cancer-associated fibroblasts; plant sap; extracellular vesicles; anti-metastatic effects; 3D microfluidics
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MDPI and ACS Style

Kim, K.; Jung, J.-H.; Yoo, H.J.; Hyun, J.-K.; Park, J.-H.; Na, D.; Yeon, J.H. Anti-Metastatic Effects of Plant Sap-Derived Extracellular Vesicles in a 3D Microfluidic Cancer Metastasis Model. J. Funct. Biomater. 2020, 11, 49. https://doi.org/10.3390/jfb11030049

AMA Style

Kim K, Jung J-H, Yoo HJ, Hyun J-K, Park J-H, Na D, Yeon JH. Anti-Metastatic Effects of Plant Sap-Derived Extracellular Vesicles in a 3D Microfluidic Cancer Metastasis Model. Journal of Functional Biomaterials. 2020; 11(3):49. https://doi.org/10.3390/jfb11030049

Chicago/Turabian Style

Kim, Kimin, Jik-Han Jung, Hye Ju Yoo, Jae-Kyung Hyun, Ji-Ho Park, Dokyun Na, and Ju Hun Yeon. 2020. "Anti-Metastatic Effects of Plant Sap-Derived Extracellular Vesicles in a 3D Microfluidic Cancer Metastasis Model" Journal of Functional Biomaterials 11, no. 3: 49. https://doi.org/10.3390/jfb11030049

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