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Open AccessArticle

In Vitro Characterization of Hypoxia Preconditioned Serum (HPS)—Fibrin Hydrogels: Basis for an Injectable Biomimetic Tissue Regeneration Therapy

1
Experimental Plastic Surgery, Department for Plastic and Hand Surgery, Klinikum rechts der Isar, Technische Universität München (TUM), D-81675 Munich, Germany
2
Department of Plastic, Reconstructive and Aesthetic Surgery, Isar Klinikum, 80331 Munich, Germany
3
Department of Plastic, Reconstructive, Hand and Burn Surgery, Bogenhausen Hospital, 81925 Munich, Germany
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Institute of Biological and Medical Imaging, Helmholtz Zentrum Munich; Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), 85764 Neuherberg, Germany
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Department for Plastic, Reconstructive and Aesthetic Surgery, Ospedale Regionale di Lugano, 6900 Lugano, Switzerland
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Department of Trauma Surgery, Orthopedics and Plastic Surgery, Universitätsmedizin Göttingen, D-37075 Göttingen, Germany
*
Author to whom correspondence should be addressed.
Both authors contributed equally to this work.
Current address: Department of Experimental Plastic Surgery, Clinic for Plastic, Reconstructive and Hand Surgery, Klinikum rechts der Isar, Technische Universität München (TUM), Ismaninger Str. 22, D-81675 Munich, Germany.
J. Funct. Biomater. 2019, 10(2), 22; https://doi.org/10.3390/jfb10020022
Received: 16 March 2019 / Revised: 26 April 2019 / Accepted: 5 May 2019 / Published: 13 May 2019
Blood-derived growth factor preparations have long been employed to improve perfusion and aid tissue repair. Among these, platelet-rich plasma (PRP)-based therapies have seen the widest application, albeit with mixed clinical results to date. Hypoxia-preconditioned blood products present an alternative to PRP, by comprising the complete wound healing factor-cascade, i.e., hypoxia-induced peripheral blood cell signaling, in addition to platelet-derived factors. This study set out to characterize the preparation of hypoxia preconditioned serum (HPS), and assess the utility of HPS–fibrin hydrogels as vehicles for controlled factor delivery. Our findings demonstrate the positive influence of hypoxic incubation on HPS angiogenic potential, and the individual variability of HPS angiogenic factor concentration. HPS–fibrin hydrogels can rapidly retain HPS factor proteins and gradually release them over time, while both functions appear to depend on the fibrin matrix mass. This offers a means of controlling factor retention/release, through adjustment of HPS fibrinogen concentration, thus allowing modulation of cellular angiogenic responses in a growth factor dose-dependent manner. This study provides the first evidence that HPS–fibrin hydrogels could constitute a new generation of autologous/bioactive injectable compositions that provide biochemical and biomaterial signals analogous to those mediating physiological wound healing. This therefore establishes a rational foundation for their application towards biomimetic tissue regeneration. View Full-Text
Keywords: hypoxia; angiogenesis; growth factor; injectable hydrogel; fibrin matrix; peripheral blood cells; tissue regeneration hypoxia; angiogenesis; growth factor; injectable hydrogel; fibrin matrix; peripheral blood cells; tissue regeneration
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Hadjipanayi, E.; Moog, P.; Bekeran, S.; Kirchhoff, K.; Berezhnoi, A.; Aguirre, J.; Bauer, A.-T.; Kükrek, H.; Schmauss, D.; Hopfner, U.; Isenburg, S.; Ntziachristos, V.; Ninkovic, M.; Machens, H.-G.; Schilling, A.F.; Dornseifer, U. In Vitro Characterization of Hypoxia Preconditioned Serum (HPS)—Fibrin Hydrogels: Basis for an Injectable Biomimetic Tissue Regeneration Therapy. J. Funct. Biomater. 2019, 10, 22.

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