What Shall We Cook Tomorrow? Empowering Students Through Sustainable Food Education and Novel Protein Exploration
Abstract
1. Introduction
2. Educational Approach
2.1. Participants and Context
2.2. Questionnaires
2.3. Teaching Activities
- A Great Food Transition;
- The Light Reactions of Photosynthesis;
- Calvin Cycle and Life Based on Carbon;
- Planetary Boundaries and Novel Food;
- Insects and Nutrition: An Eco-Sustainable Gamble.
- Biodiversity and How to Preserve It.
- Appearance and Evolution of Photosynthetic Organisms.
- Understanding the Past to Predict the Future: The Domestication of Plants.
- Towards a More Sustainable Agriculture: How to Cultivate Algae?
- Biogeochemical Cycles.
- Ocean Acidification.
- Exploring New Frontiers in Nutrition: The Importance of Omega-3 in Novel Foods.
- Determination of Fatty Acids in Food: Sample Preparation and Gas Chromatographic Analysis.
- Data Quality in Food Analysis—Part 1: Analytical Method Validation.
- Data Quality in Food Analysis—Part 2: Quantification Methods.
- Determination of Potentially Toxic Elements in Food: Sampling Strategy, Decontamination, and Materials.
- Sample Pretreatment in Food Analysis: Focus on the Mineralization Technique.
2.4. Laboratory Activities
- Preparation of liquid and solid culture media, following standard protocols. Students learned the role of each macro- and micronutrients and the main abiotic factors influencing algal growth (e.g., temperature, light, salinity).
- Inoculation of three algal species (Arthrospira platensis, Dunaliella salina, and Chlorella vulgaris) under sterile conditions, with emphasis on aseptic technique and contamination control.
- Cell counting using a hemocytometer, to quantify algal population density and introduce students to microscopic measurement techniques.
- Electrophoresis of PCR-amplified DNA fragments from spinach and algae samples, to visualize genetic material and understand the basics of molecular identification.
- Set up of insect farming on a laboratory scale, to understand the basics of rearing house crickets (Acheta domesticus) following different life stages, from hatching to adults, including cricket reproduction and egg laying.
- Protein Extraction and Sustainability Assessment: extraction and quantification of proteins from various food matrices (beef, cheese, spinach, legumes, Spirulina powder) using the Lowry method and calibration curves; estimation of environmental impact (water use, land use, CO2 equivalents) for producing 100 g of protein from different foods (e.g., beef, legumes), based on data from Our World in Data [24]; comparison with values for Spirulina as a sustainable alternative.
- Nutritional Analysis of Novel Foods: assessment of the nutritional properties of insects and algae.
- Fatty acid profile and Elemental Analysis of Insects and Algae: extraction and quantification of lipids from insect and algal samples using microwave-assisted extraction for insects and a modified Folch method for algae, followed by fatty acid profiling through GC-MS (gas chromatography–mass spectrometry); determination of potentially toxic elements (e.g., As, Cd, Pb, Hg) after microwave-assisted acid digestion, using ICP-OES (inductively coupled plasma optical emission spectrometry).
2.5. Educational Technical Videos
- Cell Counting Using a Bürker Chamber;
- Maintenance of Cyanobacteria and Diatom Cultures & Sterile Techniques;
- Preparation of a Liquid Culture Medium for Marine Algae;
- Microwave-Assisted Acid Mineralization of Insect-Based Samples.
2.6. Dissemination Activities
- Sharper Festival (European Researchers’ Night): A scientific and cultural festival held in the city centre and open to the public.
- School Presentation: A dedicated school day where participating students shared their experiences with middle school students.
- Final Event: A final event where packages of pasta, produced from locally sourced flours blended with Spirulina algae, were distributed.
3. Implementation and Outcomes of the Teaching Strategy
3.1. Advancing Perspectives on Planetary Health and Sustainable Development
3.2. Shifting Perspectives on Sustainable Dietary Practices
3.3. Student Interviews: Was the Learning Experience Transformative?
- (1)
- Taking part in the project was a wonderful experience that allowed me to discover new foods that are incredibly nutritious for humans; it was truly beautiful because we had the opportunity to closely observe how young university students work inside the laboratories.
- (2)
- I think it was truly helpful in understanding what I would like to do in the future. It offered me a perspective on career paths I never expected to learn about, and above all, it addressed a topic that concerns the future of all humanity.
- (3)
- It was a wonderful experience because it allowed us to engage in discussions both among ourselves as students and with university professors and students. So, from every point of view, it was a very educational and useful experience.
- (4)
- What struck me the most in this project were the seminars held by university professors. I believe it is very important to raise awareness about these new protein sources, because I think there is still lack of knowledge surrounding this topic. People are not fully aware of how important these alternatives to traditional food can be, and how they could represent an evolution in our nutritional culture.
- (5)
- The enthusiasm and passion of all the participants made this experience incredible. It increased my awareness of the finite nature of energy and material resources in the food sector, giving me a new perspective through more sustainable foods like crickets.
- (6)
- At first, I had doubts about the advantages insects could have compared to our traditional diet, but this project highlighted the benefits they offer over conventional meat. Honestly, I believe this is the future of humanity, and we should start breaking the stereotypes that we, as a society, continue to preserve. People are not aware of the real benefits these alternative foods can bring to our diet, making it much more diverse, rich, and environmentally friendly.
4. Transformative Potential and Future Directions
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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| Question | Options |
|---|---|
| 1. How would you define your diet? | (a) Omnivorous; (b) Pescatarian; (c) Flexitarian; (d) Vegetarian; (e) Vegan; (f) Other |
| 2. If you eat meat, which type do you mainly consume? | (a) Red meat; (b) White meat; (c) Both red and white |
| 3. How many times per week do you eat red meat? | (a) Every day; (b) 5–7 times; (c) 3–5 times; (d) 1–3 times; (e) Less than once |
| 4. If omnivorous, do you also eat processed plant-based meat substitutes? | (a) Yes; (b) No |
| 5. If yes, how often do you eat them? | (a) Every day; (b) 5–7 times; (c) 3–5 times; (d) 1–3 times; (e) Less than once |
| 6. In what way do you consume these substitutes? | (a) As an alternative to meat; (b) In addition to meat |
| 7. Have you ever eaten products containing alternative proteins derived from microalgae? | (a) Yes; (b) No; (c) I’m not sure |
| 8. Have you ever eaten products containing alternative proteins derived from insects? | (a) Yes; (b) No; (c) I’m not sure |
| 9. Do you think this project will influence your diet? | (a) Yes; (b) No; (c) I’m not sure |
| 10. Do you know the meaning of sustainability? | (a) Yes; (b) No; (c) I’m not sure |
| 11. Give a brief definition (one line or a few words) of the word sustainability | |
| 12. Do you know the meaning of planetary boundaries? | (a) Yes; (b) No; (c) I’m not sure |
| 13. Do you think the production of animal- and plant-based food contributes to greenhouse gas emissions in the atmosphere? | (a) Yes, one third of all global emissions in a year; (b) Yes, one fifth of all global emissions in a year; (c) Yes, one tenth of all global emissions in a year; (d) I’m not sure |
| 14. Do plant-based foods produce the same emissions as animal-based foods? | (a) Less; (b) More; (c) I don’t know |
| 15. How much of the edible agricultural production is intended for direct human consumption? | (a) 10%, the rest is used for feed production; (b) 90%, the rest is used for feed production; (c) Almost all of it; I don’t know |
| 16. In Europe, how much food is wasted or thrown away? | (a) One third; (b) One fifth; (c) One tenth; (d) I don’t know |
| Question | Options |
|---|---|
| 1. How would you define your diet? | (a) Omnivorous; (b) Pescatarian; (c) Flexitarian; (d) Vegetarian; (e) Vegan; (f) Other |
| 2. If you eat meat, which type do you mainly consume? | (a) Red meat; (b) White meat; (c) Both red and white |
| 3. How many times per week do you eat red meat? | (a) Every day; (b) 5–7 times; (c) 3–5 times; (d) 1–3 times; (e) Less than once |
| 4. If omnivorous, do you also eat processed plant-based meat substitutes? | (a) Yes; (b) No |
| 5. If yes, how often do you eat them? | (a) Every day; (b) 5–7 times; (c) 3–5 times; (d) 1–3 times; (e) Less than once |
| 6. In what way do you consume these substitutes? | (a) As an alternative to meat; (b) In addition to meat |
| 7. Have you ever eaten products containing alternative proteins derived from microalgae? | (a) Yes; (b) No |
| 8. Have you ever eaten products containing alternative proteins derived from insects? | (a) Yes; (b) No |
| 9. Has your diet changed since the start of this project? | (a) Yes; (b) No |
| 10. If yes, how has it changed? | (a) I eat more plant-based proteins; (b) I eat fewer plant-based proteins |
| 11. Do you think this project influenced your dietary change? | (a) Yes; (b) No |
| 12. How would you rate your experience with the project on a scale from 1 to 10? (1 = very little, 10 = a lot) | |
| 13. On a scale from 1 to 10, how engaging did you find the topics covered? (1 = very little, 10 = a lot) | |
| 14. From 1 to 10, how much do you think you’ve learned about the topics we discussed? (1 = very little, 10 = a lot) | |
| 15. From 1 to 10, how much do you think what you’ve learned will influence your lifestyle (not just your eating habits)? (1 = not at all, 10 = a lot) | |
| 16. Following what you have learned, will you try to influence the lifestyles of your household members? | (a) Yes; (b) No; (c) I am already doing so; (d) I don’t know |
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Share and Cite
Norici, A.; Truzzi, C.; Gerotto, C.; Annibaldi, A.; Illuminati, S.; Petrucciani, A.; Mollo, L.; Spinelli, G.; Minio, M.; Massi, L.; et al. What Shall We Cook Tomorrow? Empowering Students Through Sustainable Food Education and Novel Protein Exploration. Challenges 2025, 16, 53. https://doi.org/10.3390/challe16040053
Norici A, Truzzi C, Gerotto C, Annibaldi A, Illuminati S, Petrucciani A, Mollo L, Spinelli G, Minio M, Massi L, et al. What Shall We Cook Tomorrow? Empowering Students Through Sustainable Food Education and Novel Protein Exploration. Challenges. 2025; 16(4):53. https://doi.org/10.3390/challe16040053
Chicago/Turabian StyleNorici, Alessandra, Cristina Truzzi, Caterina Gerotto, Anna Annibaldi, Silvia Illuminati, Alessandra Petrucciani, Lorenzo Mollo, Graziana Spinelli, Miles Minio, Lorenzo Massi, and et al. 2025. "What Shall We Cook Tomorrow? Empowering Students Through Sustainable Food Education and Novel Protein Exploration" Challenges 16, no. 4: 53. https://doi.org/10.3390/challe16040053
APA StyleNorici, A., Truzzi, C., Gerotto, C., Annibaldi, A., Illuminati, S., Petrucciani, A., Mollo, L., Spinelli, G., Minio, M., Massi, L., Girolametti, F., Ajdini, B., Fanelli, M., Biancarosa, I., Dipietro, G., Palmucci, M., & Martino, R. (2025). What Shall We Cook Tomorrow? Empowering Students Through Sustainable Food Education and Novel Protein Exploration. Challenges, 16(4), 53. https://doi.org/10.3390/challe16040053

