Proteome Response of Meretrix Bivalves Hepatopancreas Exposed to Paralytic Shellfish Toxins Producing Dinoflagellate Gymnodinium catenatum
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals
2.2. Cultivation of Microalgae
2.3. Experimental Design
2.4. Protein Extraction from Hepatopancreas
2.5. Protein Determination
2.6. Toxin Determination
2.7. Two-Dimensional Gel Electrophoresis and Imaging Analysis
2.8. In-Gel Digestion and MALDI-TOP Mass Spectrometry Analysis
2.9. N-Terminal Sulfonation
2.10. Statistical Analysis
3. Results and Discussion
3.1. Toxin Composition in Dinoflagellate and Bivalve
3.2. Protein Profile in the Hepatopancreas of Bivalves
3.3. Biological Functions of the Identified Proteins
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Organ | Toxin Content (μg STX Equivalents Per Gram) | Relative Abundant (% Molar) |
---|---|---|
Gill | 0.74 ± 0.14 a | 1.82 |
Hepatopancreas | 38.71 ± 3.63 b | 95.27 |
Foot | 0.73 ± 0.10 a | 1.79 |
Adductor muscle | 0.212 ± 0.05 a | 0.52 |
Mantle margin | 0.107 ± 0.06 a | 0.26 |
Sighon | 0.131 ± 0.03 a | 0.32 |
Spot | pI | MW (kDa) | Fold Changes | Protein Name | Source | Amino Acid Sequence |
---|---|---|---|---|---|---|
TP1 | 4.8 | 46 | 4.2 | Response regulator receiver domain-containing protein | De novo peptide sequencing 1634.9 m/z | FAI/LAKAVNHHNWAR |
TP2 | 4.6 | 42 | 4.9 | Major facilitator superfamily transporters | De novo peptide sequencing 1154.3 m/z | AI/LFSFWI/LDR |
TP3 | 4.6 | 40 | 5.3 | Sarcoplasmic calcium-binding protein | De novo peptide sequencing 842.2 m/z | VATVSI/LPR |
TP5 | 7.0 | 60 | 2.3 | Catalase | PMF | -- |
TP6 | 6.5 | 18 | 3.2 | Protein ultraspiracle homolog | PMF | -- |
TP7 | 6.3 | 34 | 3.0 | Paramyosin | De novo peptide sequencing 1839.8 m/z | DI/LEI/LAVI/LSHESAEASI/LR |
TP8 | 6.1 | 20 | 3.8 | Mg superoxide dismutase | De novo peptide sequencing 1646.6 m/z | AI/LFKI/LANWEEVGNR |
TP9 | 4.5 | 29 | 3.3 | G2 and S phase-expressed protein 1 | PMF | -- |
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Chan, K.-K.; Tam, N.F.-Y.; Ng, C.; Kwok, C.S.-N.; Xu, S.J.-L.; Sze, E.T.-P.; Lee, F.W.-F. Proteome Response of Meretrix Bivalves Hepatopancreas Exposed to Paralytic Shellfish Toxins Producing Dinoflagellate Gymnodinium catenatum. J. Mar. Sci. Eng. 2021, 9, 1039. https://doi.org/10.3390/jmse9091039
Chan K-K, Tam NF-Y, Ng C, Kwok CS-N, Xu SJ-L, Sze ET-P, Lee FW-F. Proteome Response of Meretrix Bivalves Hepatopancreas Exposed to Paralytic Shellfish Toxins Producing Dinoflagellate Gymnodinium catenatum. Journal of Marine Science and Engineering. 2021; 9(9):1039. https://doi.org/10.3390/jmse9091039
Chicago/Turabian StyleChan, Kin-Ka, Nora Fung-Yee Tam, Christie Ng, Celia Sze-Nga Kwok, Steven Jing-Liang Xu, Eric Tung-Po Sze, and Fred Wang-Fat Lee. 2021. "Proteome Response of Meretrix Bivalves Hepatopancreas Exposed to Paralytic Shellfish Toxins Producing Dinoflagellate Gymnodinium catenatum" Journal of Marine Science and Engineering 9, no. 9: 1039. https://doi.org/10.3390/jmse9091039