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Open AccessArticle

Induced Systemic Resistance (ISR) in Arabidopsis thaliana by Bacillus amyloliquefaciens and Trichoderma harzianum Used as Seed Treatments

1
Laboratory of Phytopathology, Department of Production, Protection and Biotechnology, Agronomic and Veterinary Institute Hassan II, Rabat 10010, Morocco
2
IRHS, INRA-University of Angers-Agrocampus-Ouest, Angers, SFR 4207 QuaSav, 49071 Beaucouze, France
3
Laboratory of Phytopathology and Post-Harvest Quality, Regional Centre for Agronomic Research, Kenitra 14070, Morocco
*
Author to whom correspondence should be addressed.
Agriculture 2019, 9(8), 166; https://doi.org/10.3390/agriculture9080166
Received: 6 June 2019 / Revised: 17 July 2019 / Accepted: 19 July 2019 / Published: 1 August 2019
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Abstract

The Trichoderma fungal species and the bacteria Bacillus species were described as inducers of plant systemic resistance in relation to their antagonistic activity. The objective of this study was to evaluate the effect of selected strains of Bacillus amyloliquefaciens (I3) and Trichoderma harzianum (A) on inducing systemic resistance in Arabidopsis thaliana as a model for plant molecular genetics. The microorganisms were identified and were confirmed for their antagonistic potential in vitro and in vivo in previous studies. In order to explore this mechanism, two mutants of A. thaliana carrying a PR1 promoter (a conventional marker of salicylic acid (SA) pathway) and LOX2 promoter (a marker triggering jasmonic acid (JA) pathway activation) were analyzed after inoculating antagonists. Transgenic reporter line analysis demonstrated that B. amyloliquefaciens I3 and T. harzianum A induce A. thaliana defense pathways by activating SA and JA at a high level compared to lines treated with chemical elicitors of references (acibenzolar-S-methyl (Bion 50 WG (water-dispersible granule)), SA, and methyl jasmonate). The efficacy of B. amyloliquefaciens I3 and T. harzianum A in inducing the defense mechanism in A. thaliana was demonstrated in this study. View Full-Text
Keywords: seed treatment; Bacillus amyloliquefaciens I3; Trichoderma harzianum A; salicylic acid; jasmonic acid; Arabidopsis thaliana seed treatment; Bacillus amyloliquefaciens I3; Trichoderma harzianum A; salicylic acid; jasmonic acid; Arabidopsis thaliana
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MDPI and ACS Style

Ilham, B.; Noureddine, C.; Philippe, G.; Mohammed, E.G.; Brahim, E.; Sophie, A.; Martine, N.; Muriel, M. Induced Systemic Resistance (ISR) in Arabidopsis thaliana by Bacillus amyloliquefaciens and Trichoderma harzianum Used as Seed Treatments. Agriculture 2019, 9, 166.

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