Next Article in Journal
A Time-Series Analysis of Climate Variability in Urban and Agricultural Sites (Rome, Italy)
Next Article in Special Issue
The Effect of Herbage Conservation Method on Protein Value and Nitrogen Utilization in Dairy Cows
Previous Article in Journal
Is a Change of Protein Composition after Late Application of Nitrogen Sufficient to Improve the Baking Quality of Winter Wheat?
Previous Article in Special Issue
Utilization of Molecular Marker Based Genetic Diversity Patterns in Hybrid Parents to Develop Better Forage Quality Multi-Cut Hybrids in Pearl Millet
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle

Effect of Species, Fertilization and Harvest Date on Microbial Composition and Mycotoxin Content in Forage

1
Mendel University in Brno, Faculty of AgriSciences, Department of Animal Nutrition and Forage Production, Zemedelska 3, 613 00 Brno, Czech Republic
2
Department of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, Zemedelska 3, 613 00 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Agriculture 2019, 9(5), 102; https://doi.org/10.3390/agriculture9050102
Received: 25 March 2019 / Revised: 29 April 2019 / Accepted: 2 May 2019 / Published: 6 May 2019
(This article belongs to the Special Issue Quality and Production of Forage)
  |  
PDF [232 KB, uploaded 6 May 2019]
  |  

Abstract

The aim of the project was to evaluate the potential of microbial threat to feed safety in the year 2018. Analyses of the epiphytic community of several forage species (clovers, cocksfoot, fescue, festulolium, perennial ryegrass, timothy and trefoil) in variants of fertilized and non-fertilized vegetation were performed. The hypothesis is based on the fact that microorganisms are normally present on plant material during its growth all the way from the seed to the senescence; they are influenced by a plant’s fitness, and they affect its harvest and utilization. Microflora was analyzed by cultivation on specific substrates, total microbial count and five specific microbial groups were observed and quantified. Forage species did not affect plant microflora. The highest risk factor of microbial contamination of feed was proved to be harvest date. Mycotoxin contamination of fresh feed was determined (deoxynivalenol and zearalenone) using ELISA. Zearalenone (ZEA) levels were negatively correlated to fertilization intensity, although these results were not statistically significant. Deoxynivalenol (DON) levels were the lowest in a moderate fertilization regime. Significant differences in mycotoxin content were found among botanical species. View Full-Text
Keywords: grass; clover; epiphytic microflora; fungi; deoxynivalenol; zearalenone grass; clover; epiphytic microflora; fungi; deoxynivalenol; zearalenone
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Baholet, D.; Kolackova, I.; Kalhotka, L.; Skladanka, J.; Haninec, P. Effect of Species, Fertilization and Harvest Date on Microbial Composition and Mycotoxin Content in Forage. Agriculture 2019, 9, 102.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Agriculture EISSN 2077-0472 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top