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Agriculture 2018, 8(3), 36; https://doi.org/10.3390/agriculture8030036

A Protocol for Producing Virus-Free Artichoke Genetic Resources for Conservation, Breeding, and Production

1
Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari “Aldo Moro”, Via Amendola 165/A, 70126 Bari, Italy
2
Istituto per la Protezione Sostenibile delle Piante (IPSP), CNR, UOS Bari, Via Amendola 122/D, 70126 Bari, Italy
*
Author to whom correspondence should be addressed.
Received: 30 January 2018 / Revised: 21 February 2018 / Accepted: 27 February 2018 / Published: 1 March 2018
(This article belongs to the Special Issue Biodiversity of Vegetable Crops, A Living Heritage)
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Abstract

The potential of the globe artichoke biodiversity in the Mediterranean area is enormous but at risk of genetic erosion because only a limited number of varieties are vegetatively propagated and grown. In Apulia (southern Italy), the Regional Government launched specific actions to rescue and preserve biodiversity of woody and vegetable crops in the framework of the Rural Development Program. Many globe artichoke ecotypes have remained neglected and unnoticed for a long time and have been progressively eroded by several causes, which include a poor phytosanitary status. Sanitation of such ecotypes from infections of vascular fungi and viruses may be a solution for their ex situ conservation and multiplication in nursery plants in conformity to the current EU Directives 93/61/CEE and 93/62/CEE that enforce nursery productions of virus-free and true-to-type certified stocks. Five Apulian ecotypes, Bianco di Taranto, Francesina, Locale di Mola, Verde di Putignano and Violetto di Putignano, were sanitized from artichoke Italian latent virus (AILV), artichoke latent virus (ArLV) and tomato infectious chlorosis virus (TICV) by meristem-tip culture and in vitro thermotherapy through a limited number of subcultures to reduce the risk of “pastel variants” induction of and loss of earliness. A total of 25 virus-free primary sources were obtained and conserved ex situ in a nursery. View Full-Text
Keywords: artichoke; ecotype; virus-sanitation; meristem-tip culture; thermotherapy artichoke; ecotype; virus-sanitation; meristem-tip culture; thermotherapy
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Spanò, R.; Bottalico, G.; Corrado, A.; Campanale, A.; Di Franco, A.; Mascia, T. A Protocol for Producing Virus-Free Artichoke Genetic Resources for Conservation, Breeding, and Production. Agriculture 2018, 8, 36.

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