Next Article in Journal
Detection of Key Organs in Tomato Based on Deep Migration Learning in a Complex Background
Next Article in Special Issue
Drivers of Personal Safety in Agriculture: A Case Study with Pesticide Operators
Previous Article in Journal
The Determinants of Large-Scale Land Acquisitions (LSLAs) in Sub-Saharan Africa (SSA): A Case Study
Previous Article in Special Issue
Monitoring of Pesticides in the Cultivation of Nopal Vegetable (Opuntia ficus-indica (L.)) Mill, Morelos, México
Open AccessReview

Methods for the Diagnosis of Grapevine Viral Infections: A Review

1
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, Moscow 119071, Russia
2
Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, Moscow 119071, Russia
*
Author to whom correspondence should be addressed.
Agriculture 2018, 8(12), 195; https://doi.org/10.3390/agriculture8120195
Received: 26 September 2018 / Revised: 28 November 2018 / Accepted: 5 December 2018 / Published: 9 December 2018
(This article belongs to the Special Issue Pesticides in Agriculture System)
The yielding capacity of grapevine growth and the quality of the resulting product heavily depend on the health of the cultivated plants. The phytopathogens affecting the vineyards can cause a significant reduction in the yield and quality of the product. For this reason, it is extremely important to use diagnostic methods that make it possible to identify the pathogens, and to choose the correct method of plant protection. This review considers the main viral grapevine pathogens, and the existing methods of their diagnosis. The limitations of conventional diagnostic methods that are based either on the visual assessment of symptoms, or on bio-testing, are analyzed. A major focus is placed on two intensively developed approaches of diagnosis, molecular genetic and immunochemical methods. Applications of amplification techniques and DNA chips are presented, as well as opportunities for next-generation sequencing. A reduction of assay duration and labor intensity in combination with the assay shifts from specialized laboratories toward the places of sampling are considered as the main factors influencing the development of immunodiagnostic techniques. The potential place of diagnostic tests in vine-growing practices, and the requirements for their most efficient applications for early disease diagnosis is also discussed. View Full-Text
Keywords: grapevine; viral infections; phytopathogens; molecular diagnosis; immunodiagnosis grapevine; viral infections; phytopathogens; molecular diagnosis; immunodiagnosis
MDPI and ACS Style

Zherdev, A.V.; Vinogradova, S.V.; Byzova, N.A.; Porotikova, E.V.; Kamionskaya, A.M.; Dzantiev, B.B. Methods for the Diagnosis of Grapevine Viral Infections: A Review. Agriculture 2018, 8, 195.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop