Mycobiome and Pathogenic Fusarium Fungi in the Rhizosphere of Durum Wheat After Seed Dressing with Debaryomyces hansenii
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsThis research results are innovative, and some problems need to be revised.
1. P163， Fusarium should be italic. Please double-check.
2. Table 3 and 4 should be unified in three-line form.
3. Seed dressing or seed coating , chose one of them may be better.
4. P495， The journal name should be 'mBio'. Please double-check.
5. There is a lack of discussion on the mechanism of ' how rhizosphere fungal community dynamics affect plant disease resistance ', and relevant theoretical support ( such as plant immune induction mechanism ) needs to be cited.
6. It is necessary to explain why Durasol and Floradur were selected, and whether there was a difference in resistance ?
Comments on the Quality of English LanguageNo.
Author Response
The authors would like to thank for a positive review. All corrections in the manuscript were made according to the reviewer's suggestions.
Rev. 1
This research results are innovative, and some problems need to be revised.
- P163， Fusarium should be italic. Please double-check.
Response 1: Corrected: Fusarium (italic)
- Table 3 and 4 should be unified in three-line form.
Response 2: Corrected
- Seed dressing or seed coating , chose one of them may be better.
Response 3:Corrected L12 and L.21, L. 287, L. 450, L. 452: “seed dressing” was chosen.
- P495， The journal name should be 'mBio'. Please double-check.
Response 4:Corrected: mBio
- There is a lack of discussion on the mechanism of ' how rhizosphere fungal community dynamics affect plant disease resistance ', and relevant theoretical support ( such as plant immune induction mechanism ) needs to be cited.
Response 5: the text is supplemented here:
Earlier Sevillano-Caño et al. [53] showed that D. hansenii induced the expression of several genes related to ET biosynthesis (ACO1), ET signalling (EIN2 and EIN3), and SA biosynthesis (PAL) in cucumber leaves from plants grown in soil inoculated with D. hansenii. Sevillano-Caño et al. [53] suggest that D. hansenii can induce both induced systemic resistance (ISR) and systemic acquired resistance (SAR). In this way D. hansenii can increase plant resistance to the pathogen infections, initiate in the plants a state known as priming. This phenomenon was described by Pieterse et al. (2014) as a plants response to rhizosphere microorganisms.
The latest papers on the induction of plant resistance as a result of D.hansei yeast application were added to the literature chapter.
{55] Sevillano-Caño J, García MJ, Córdoba-Galván C, Luque-Cruz C, Agustí-Brisach C, Lucena C, Ramos J, Pérez-Vicente R, Romera FJ. Exploring the role of Debaryomyces hansenii as biofertilizer in iron-deficient environments to enhance plant nutrition and crop production sustainability. Int J Mol Sci. 24; 25(11):5729. doi: 10.3390/ijms25115729.
and
[56] Pieterse C.M.J., Zamioudis C., Berendsen R.L., Weller D.M., Van Wees S.C.M., Bakker P.A.H.M. Induced systemic resistance by beneficial microbes. Annu. Rev. Phytopathol. 2014;52:347–375. doi: 10.1146/annurev-phyto-082712-102340.
- It is necessary to explain why Durasol and Floradur were selected, and whether there was a difference in resistance ?
Response 6: These cultivar have a different resistance level to Fusarium head blight (FHB): Durasol cultivar is less resistant (level 4) but Floradur cultivar is more resistant (6).
the text is supplemented here L.365-366: ” ….(spring cvs. Floradur – more resistant and Durasol - less resistant, [56]), ..”
Reviewer 2 Report
Comments and Suggestions for AuthorsThis manuscript addresses a highly relevant topic in the field of agricultural biotechnology, analyzing the impact of seed treatment with microbial suspensions on crop health and pathogen control, offering a promising perspective on ecological alternatives to pesticides.
To improve this article, I have several recommendations:
Abstract: Mention the two wheat varieties included in the study.
Introduction: I recommend that the authors include information on the antagonistic interaction between Debaryomyces hansenii and Fusarium (e.g., the production of volatile compounds influencing germination and mycelial growth, competition for nutrients and space, and the induction of plant resistance). To support the authors in this regard, I suggest consulting the following articles:
- Huang, Chuchu, Zhang, Ling, Johansen, Pernille Greve, Petersen, Mikael Agerlin, Arneborg, Nils, & Jespersen, Lene. Debaryomyces hansenii Strains Isolated From Danish Cheese Brines Act as Biocontrol Agents to Inhibit Germination and Growth of Contaminating Molds. Frontiers in Microbiology, Volume 12, 2021.
- Rivas-Garcia T, Murillo-Amador B, Reyes-Pérez JJ, Chiquito-Contreras RG, Preciado-Rangel P, Ávila-Quezada GD, Lara-Capistran L, Hernandez-Montiel LG. Debaryomyces hansenii, Stenotrophomonas rhizophila, and Ulvan as Biocontrol Agents of Fruit Rot Disease in Muskmelon (Cucumis melo L.). Plants (Basel). 2022 Jan 11;11(2):184.
Materials and Methods:
- Why were the wheat varieties Floradur and Durasol chosen? Provide justification.
- Add information about the control treatment (Was it untreated? Uninfected?).
Results:
- Why did the fungicide treatment lead to a greater reduction in diversity in the plots sown with Durasol compared to those sown with Floradur?
- Why does the final yield not show significant differences in the fungicide-treated variant if NDVI and the number of spikes/m² were significantly improved?
Bibliography:
- Reference number 56 is missing from the text – it is not cited in the article (ensure proper citation formatting).
- There are self-citations, but they are relevant to the topic.
Author Response
Rev 2
The authors would like to thank for a positive review. All corrections in the manuscript were made according to the reviewer's suggestions.
This manuscript addresses a highly relevant topic in the field of agricultural biotechnology, analyzing the impact of seed treatment with microbial suspensions on crop health and pathogen control, offering a promising perspective on ecological alternatives to pesticides.
To improve this article, I have several recommendations:
- Abstract: Mention the two wheat varieties included in the study.
Response 1: L.15. added here “ ….of durum wheat cultivars (Durasol and Floradur)…”
- Introduction: I recommend that the authors include information on the antagonistic interaction between Debaryomyces hansenii and Fusarium (e.g., the production of volatile compounds influencing germination and mycelial growth, competition for nutrients and space, and the induction of plant resistance). To support the authors in this regard, I suggest consulting the following articles:
- Huang, Chuchu, Zhang, Ling, Johansen, Pernille Greve, Petersen, Mikael Agerlin, Arneborg, Nils, & Jespersen, Lene. Debaryomyces hansenii Strains Isolated From Danish Cheese Brines Act as Biocontrol Agents to Inhibit Germination and Growth of Contaminating Molds. Frontiers in Microbiology, Volume 12, 2021.
- Rivas-Garcia T, Murillo-Amador B, Reyes-Pérez JJ, Chiquito-Contreras RG, Preciado-Rangel P, Ávila-Quezada GD, Lara-Capistran L, Hernandez-Montiel LG. (Cucumis melo L.). Plants (Basel). 2022 Jan 11;11(2):184.
Response 2: the text is supplemented here: The yeast D. hansenii has shown significant results as a biocontrol agent by diverse mechanisms of action, such as competition for space (i.e. inhibition of spore germination) and nutrients, and secondary metabolite excretion (i.e. volatile organic compounds) or induction of antioxidant enzymes in muskmelon to reduce the rot caused by F. proliferatum.
These paper were cited:
Huang C., Zhang L., Johansen P.G., Petersen M.A., Arneborg N., Jespersen L. Debaryomyces hansenii strains isolated from danish cheese brines act as biocontrol agents to inhibit germination and growth of contaminating molds. Front Microbiol. 2021,12, 662785. doi: 10.3389/fmicb.2021.662785.
Rivas-Garcia, T., Murillo-Amador, B., Reyes-Pérez, J. J., Chiquito-Contreras, R. G., Preciado-Rangel, P., Ávila-Quezada, G. D., ... & Hernandez-Montiel, L. G. (2022). Debaryomyces hansenii, Stenotrophomonas rhizophila, and ulvan as biocontrol agents of fruit rot disease in muskmelon (Cucumis melo L.). Plants, 11(2), 184. https://doi.org/10.3390/plants11020184
- Materials and Methods:
- Why were the wheat varieties Floradur and Durasol chosen? Provide justification.
Response 3:These cultivar have a different resistance level to Fusarium head blight (FHB): Durasol cultivar is less resistant (level 4) but Floradur cultivar is more resistant (6).
- the text is supplemented here 365-366: ” ….(spring cvs. Floradur – more resistant and Durasol - less resistant, [56]), ..”
- 4. Add information about the control treatment (Was it untreated? Uninfected?).
Response 4: added here: The control treatment were plants growth from seeds untreated and uninfected.
- Results:
- Why did the fungicide treatment lead to a greater reduction in diversity in the plots sown with Durasol compared to those sown with Floradur?
Response 5: This was probably the effect of differences in the microbiome between these varieties i.e. in the control treatment 172 fungi species occurred on the Floradur plants while on the Durasol plants only 153. It is possible that a part of fungi species occurred on the Durasol plant were more sensitive to the fungicide.
- 6. Why does the final yield not show significant differences in the fungicide-treated variant if NDVI and the number of spikes/m² were significantly improved?
Response 6:The yield from the fungicide treated plots was on average higher than the yield from control plots. It was not confirmed by statistical analysis, probably due to the influence the various factors for this parameter.
Bibliography:
- 7. Reference number 56 is missing from the text – it is not cited in the article (ensure proper citation formatting).
- Response 7: Corrected and supplemented with 4 publications.
- There are self-citations, but they are relevant to the topic.
