OsHSP 17.9, a Small Heat Shock Protein, Confers Improved Productivity and Tolerance to High Temperature and Salinity in a Natural Paddy Field in Transgenic Rice Plants
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Stress Treatments
2.2. Genomic DNA Isolation and PCR Analysis
2.3. Analysis of Semi-Quantitative RT-PCR
2.4. Measurement of Accumulative ROS Content
2.5. Evaluation of Early Growth in a Natural Paddy Field and Ion Leakage in Response to Methyl Viologen (MV)
2.6. Antioxidant Enzyme Assay
2.7. Evaluation of Early Growth and Agronomic Traits in a Natural Paddy Field
2.8. Statistical Analysis
3. Results
3.1. Production of OsHSP 17.9 Gene Transgenic Plants
3.2. Comparison of Phenotype and OsHSP 17.9 Gene Expression Levels in Salt and High-Temperature Stress
3.3. Analysis of Oxidative Stress Tolerance through Accumulated MDA and H2O2 Content
3.4. Early Growth and Environmental Adaptability in a Natural Paddy Field and Ion Leakage in Response to MV
3.5. Enhanced Antioxidant Enzyme Activity of Transgenic Plants Grown in a Natural Paddy Field
3.6. Grain Yield and Agronomic Analysis in Natural Paddy-Field Conditions
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Primer Set | 5′-3′ |
---|---|
OsHSP-F2 | AGCATCTTCCCGTCCTTCC |
OsHSP-R4 | TGACCTCCTCCTTCTTCAGC |
OsHSP-26.7-F | CAGGAGAACAGGGACAACAC |
OsHSP-26.7-R | CCATCGTGTCCAGCATCT |
OsHSP-23.2-F | ATGGCGTCGATGAGAACT |
OsHSP-23.2-R | AGGTCCTCCTTCCTCATCC |
OsHSP-18.1-F | AAGGAGCAGGAGGAGAAGAC |
OsHSP-18.1-R | TAACCTGGATGGACTTGACG |
OsHSP-17.7-F | AGGAGGAGAAGTCGGACAAG |
OsHSP-17.7-R | AGATCTGGATGGACTTGACG |
OsHSP-16.9-F | GAAGGAGGACAAGAACGACA |
OsHSP-16.9-R | TTAACCGGAGATCTCAATGG |
Tub-F | GAGTACCCTGACCGCATGAT |
Tub-R | GTGGTCAGCTTGAGAGTCCT |
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Do, J.-M.; Kim, H.-J.; Shin, S.-Y.; Park, S.-I.; Kim, J.-J.; Yoon, H.-S. OsHSP 17.9, a Small Heat Shock Protein, Confers Improved Productivity and Tolerance to High Temperature and Salinity in a Natural Paddy Field in Transgenic Rice Plants. Agriculture 2023, 13, 931. https://doi.org/10.3390/agriculture13050931
Do J-M, Kim H-J, Shin S-Y, Park S-I, Kim J-J, Yoon H-S. OsHSP 17.9, a Small Heat Shock Protein, Confers Improved Productivity and Tolerance to High Temperature and Salinity in a Natural Paddy Field in Transgenic Rice Plants. Agriculture. 2023; 13(5):931. https://doi.org/10.3390/agriculture13050931
Chicago/Turabian StyleDo, Jeong-Mi, Hee-Jin Kim, Sun-Young Shin, Seong-Im Park, Jin-Ju Kim, and Ho-Sung Yoon. 2023. "OsHSP 17.9, a Small Heat Shock Protein, Confers Improved Productivity and Tolerance to High Temperature and Salinity in a Natural Paddy Field in Transgenic Rice Plants" Agriculture 13, no. 5: 931. https://doi.org/10.3390/agriculture13050931