Allelic Variation of Puroindolines Genes in Iranian Common Wheat Landraces
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Genomic DNA Extraction
2.3. Amplification and Digestion of Pina-D1 and Pinb-D1 Genes
2.4. Cloning of Pinb-D1 Genes
3. Results
3.1. Variation in Grain Hardness
3.2. PCR Analysis of the Pina-D1 and Pinb-D1 Genes
3.3. Enzymatic Digestion and Sequencing of Pinb-D1 Alleles
3.4. Amino Acid Sequence Analysis and Relationship with Grain Hardness
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Allele | Mutation | N | Reference |
---|---|---|---|
Pinb-D1b | Gly/Ser at position 46 | 11 | [6,26] |
Pinb-D1ab | Gln/Stop at position 382 | 175 | [17,26] |
Pinb-D1p | Lsy/Asn at position 210 | 80 | [27,30] |
Pinb-D1e | Trp/Stop at position 39 | 0 | [26,31] |
Pinb-D1c | Leu/Pro at position 60 | 0 | [14] |
Puroindoline Composition | N | PSI (% ± s.d.) | Range |
---|---|---|---|
Pina-D1a/Pinb-D1b | 11 | 45.91 ± 3.58 AB* | 40–50 |
Pina-D1a/Pinb-D1ab | 175 | 47.13 ± 2.70 A | 40–50 |
Pina-D1a/Pinb-D1p | 80 | 47.48 ± 2.64 A | 41–50 |
Pina-D1a/Pinb-D1ak | 5 | 44.20 ± 2.93 B | 41–49 |
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Huertas-García, A.B.; Guzmán, C.; Tabbita, F.; Alvarez, J.B. Allelic Variation of Puroindolines Genes in Iranian Common Wheat Landraces. Agriculture 2022, 12, 1196. https://doi.org/10.3390/agriculture12081196
Huertas-García AB, Guzmán C, Tabbita F, Alvarez JB. Allelic Variation of Puroindolines Genes in Iranian Common Wheat Landraces. Agriculture. 2022; 12(8):1196. https://doi.org/10.3390/agriculture12081196
Chicago/Turabian StyleHuertas-García, Ana B., Carlos Guzmán, Facundo Tabbita, and Juan B. Alvarez. 2022. "Allelic Variation of Puroindolines Genes in Iranian Common Wheat Landraces" Agriculture 12, no. 8: 1196. https://doi.org/10.3390/agriculture12081196
APA StyleHuertas-García, A. B., Guzmán, C., Tabbita, F., & Alvarez, J. B. (2022). Allelic Variation of Puroindolines Genes in Iranian Common Wheat Landraces. Agriculture, 12(8), 1196. https://doi.org/10.3390/agriculture12081196