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Article

Evaluation of a Novel Molecular Marker Associated with the Tan Spot Disease Response in Wheat

Centre for Crop and Disease Management, School of Molecular Life Sciences, Curtin University, Bentley, WA 6102, Australia
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Author to whom correspondence should be addressed.
Academic Editors: Patricia Giraldo and José Miguel Soriano
Agriculture 2021, 11(6), 513; https://doi.org/10.3390/agriculture11060513
Received: 25 March 2021 / Revised: 25 May 2021 / Accepted: 26 May 2021 / Published: 1 June 2021
(This article belongs to the Special Issue Genetics, Genomics, and Genetic Improvement of Wheat)
After nearly 40 years of DNA molecular marker development in plant breeding, the wheat research community has amassed an extensive collection of molecular markers which have been widely and successfully used for selection of agronomic, physiological and disease resistance traits in wheat breeding programs. Tan spot is a major fungal disease of wheat and a significant global economic challenge and is caused by the necrotrophic fungal pathogen Pyrenophora tritici-repentis (Ptr). Here, the potential for using a PCR-based marker (Ta1AS3422) present on the short arm of wheat chromosome 1A, was evaluated for effectiveness in distinguishing tan spot disease susceptibility. The marker was initially screened against 40 commercial Australian hexaploid wheat varieties, and those that amplified the marker had an overall lower disease score (2.8 ± 0.7 for seedlings and 2.4 ± 0.4 for plants at the tillering stage), compared to those lacking the marker which exhibited a higher disease score (3.6 ± 0.8 for both growth stages). The potential of Ta1AS3422 as a marker for the tan spot disease response was further assessed against a panel of 100 commercial Australian hexaploid wheat varieties. A significant association was observed between marker absence/presence and tan spot disease rating (Pearson’s chi-squared test, χ2 (6) = 20.53, p = 0.002), with absence of Ta1AS3422 associated with susceptibility. This simple and cost-effective PCR-based marker may be useful for varietal improvement against tan spot, although further work is required to validate its effectiveness. View Full-Text
Keywords: yellow spot; SSR; microsatellite; Pyrenophora tritici-repentis; ToxA; wheat breeding yellow spot; SSR; microsatellite; Pyrenophora tritici-repentis; ToxA; wheat breeding
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MDPI and ACS Style

See, P.T.; Moffat, C.S. Evaluation of a Novel Molecular Marker Associated with the Tan Spot Disease Response in Wheat. Agriculture 2021, 11, 513. https://doi.org/10.3390/agriculture11060513

AMA Style

See PT, Moffat CS. Evaluation of a Novel Molecular Marker Associated with the Tan Spot Disease Response in Wheat. Agriculture. 2021; 11(6):513. https://doi.org/10.3390/agriculture11060513

Chicago/Turabian Style

See, Pao T., and Caroline S. Moffat 2021. "Evaluation of a Novel Molecular Marker Associated with the Tan Spot Disease Response in Wheat" Agriculture 11, no. 6: 513. https://doi.org/10.3390/agriculture11060513

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