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Article

Biosystematic Study on Some Egyptian Species of Astragalus L. (Fabaceae)

1
Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza 12613, Egypt
2
Department of Botany and Microbiology, Faculty of Science, Zagazig University, Al Sharqiya Governorate 44519, Egypt
3
Department of Botany and Microbiology, Faculty of Science, Benha University, Benha 13518, Egypt
4
Department of Botany, Faculty of Science, Ain Shams University, Cairo 11566, Egypt
*
Author to whom correspondence should be addressed.
Academic Editors: Robert Jarret and Maarten Van Zonneveld
Agriculture 2021, 11(2), 125; https://doi.org/10.3390/agriculture11020125
Received: 31 December 2020 / Revised: 14 January 2021 / Accepted: 22 January 2021 / Published: 4 February 2021
(This article belongs to the Special Issue Conservation and Characterization of Vegetable Crop Biodiversity)
Astragalus L. is one of the largest angiosperm complex genera that belongs to the family Fabaceae, subfamily Papilionoideae or Faboideae under the subtribe Astragalinae of the tribe Galegeae. The current study includes the whole plant morphology, DNA barcode (ITS2), and molecular marker (SCoT). Ten taxa representing four species of Astragalus were collected from different localities in Egypt during the period from February 2018 to May 2019. Morphologically, identification and classification of collected Astragalus plants occurred by utilizing the light microscope, regarding the taxonomic revisions of the reference collected Astragalus specimens in other Egyptian Herbaria. For molecular validation, ten SCoT primers were used in this study, producing a unique banding pattern to differentiate between ten samples of Astragalus taxa which generated 212 DNA fragments with an average of 12.2 bands per 10 Astragalus samples, with 8 to 37 fragments per primer. The 212 fragments amplified were distributed as 2 monomorphic bands, 27 polymorphic without unique bands, 183 unique bands (210 Polymorphic with unique bands), and ITS2 gene sequence was showed as the optimal barcode for identifying Astragalus L. using BLAST searched on NCBI database, and afterward, analyzing the chromatogram for ITS region, 10 samples have been identified as two samples representing A. hauarensis, four samples representing A. sieberi, three samples representing A. spinosus and one sample representing A. vogelii. Based on the ITS barcode, A. hauarensis RMG1, A. hauarensis RMG2, A. sieberi RMG1, A. sieberi RMG2, A. sieberi RMG3, A. sieberi RMG4, A. spinosus RMG1, A. spinosus RMG2, A. spinosus RMG3, A. vogelii RMG were deposited into GenBank with accession # MT367587.1, MT367591.1, MT367593.1, MT367585.1, MT367586.1, MT367588.1, MT160347.1, MT367590.1, MT367589.1, MT367592.1, respectively. These results indicated the efficiency of SCoT markers and ITS2 region in identifying and determining genetic relationships between Astragalus species. View Full-Text
Keywords: morphology; DNA extraction; SCoT polymorphism; ITS2 region; phylogeny morphology; DNA extraction; SCoT polymorphism; ITS2 region; phylogeny
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MDPI and ACS Style

Abd El-Ghani, M.M.; El-Sayed, A.S.A.; Moubarak, A.; Rashad, R.; Nosier, H.; Khattab, A. Biosystematic Study on Some Egyptian Species of Astragalus L. (Fabaceae). Agriculture 2021, 11, 125. https://doi.org/10.3390/agriculture11020125

AMA Style

Abd El-Ghani MM, El-Sayed ASA, Moubarak A, Rashad R, Nosier H, Khattab A. Biosystematic Study on Some Egyptian Species of Astragalus L. (Fabaceae). Agriculture. 2021; 11(2):125. https://doi.org/10.3390/agriculture11020125

Chicago/Turabian Style

Abd El-Ghani, Monier M., Ashraf S. A. El-Sayed, Ahmed Moubarak, Rabab Rashad, Hala Nosier, and Adel Khattab. 2021. "Biosystematic Study on Some Egyptian Species of Astragalus L. (Fabaceae)" Agriculture 11, no. 2: 125. https://doi.org/10.3390/agriculture11020125

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