Evaluating Multiple Allelic Combination to Determine Tiller Angle Variation in Rice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Measurement of Tiller Angle
2.2. Development of Single-Nucleotide Polymorphism (SNP) Markers and PCR Condition
2.3. Development of Linear Regression Equation Model and Statistical Analysis
3. Results
3.1. Variation of Tiller Angle in Rice Germplasms
3.2. Development of SNP Markers Associated with Tiller Angle
3.3. Development of a Multiple Linear Regression Model for Tiller Angle Using a Model Rice Population
3.4. Test of a Multiple Linear Regression Model for Rice Tiller Angle Using a Test Population
3.5. Allelic Combinations of Rice Tiller Angle-Related Markers in a Test Population
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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QTL/Gene | SNP Marker | Binary Code of Allele | Estimate | Standard Deviation | t-Value | |
---|---|---|---|---|---|---|
1 | 0 | |||||
OsPIN2 | PIN2-1 | G (N) | A (W) | −8.4 | 1.06 | −7.92 *** |
OsLIC1 | LIC1-1 | G (W) | A (N) | 2.93 | 0.5 | 5.82 *** |
LIC1-2 | A (N) | G (W) | −1.96 | 0.66 | −2.97 *** | |
D3 | D3-2 | T (N) | C (W) | −1.5 | 0.37 | −4.09 *** |
D17 | D17-2 | T (W) | C (N) | 6.6 | 1.13 | 5.83 *** |
qTA8a | qTA8a-1 | G (W) | T (N) | 4.64 | 1.08 | 4.31 *** |
TAC3 | TAC3 | A (W) | T (N) | 1.68 | 0.81 | 2.06 * |
TAC1 | TAC1 | C (N) | T (W) | −3.24 | 0.91 | −3.58 *** |
Intercept | 5.29 | 0.97 | 5.48 *** | |||
Regression equation | Tiller angle = 5.29 − 8.4 (PIN2-1) + 2.93 (LIC1-1) − 1.96 (LIC1-2) − 1.5 (D3-2) + 6.6 (D17-2) + 4.64 (qTA8a-1) + 1.68 (TAC3) − 3.24 (TAC1) | |||||
R2 | 0.53 | |||||
Adjusted R2 | 0.51 | |||||
F-statistic | 36.92 | |||||
p-value | <0.001 |
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Jang, S.; Kang, Y.S.; Lee, Y.K.; Koh, H.-J. Evaluating Multiple Allelic Combination to Determine Tiller Angle Variation in Rice. Agriculture 2020, 10, 428. https://doi.org/10.3390/agriculture10100428
Jang S, Kang YS, Lee YK, Koh H-J. Evaluating Multiple Allelic Combination to Determine Tiller Angle Variation in Rice. Agriculture. 2020; 10(10):428. https://doi.org/10.3390/agriculture10100428
Chicago/Turabian StyleJang, Su, Yoo Seok Kang, Yoon Kyung Lee, and Hee-Jong Koh. 2020. "Evaluating Multiple Allelic Combination to Determine Tiller Angle Variation in Rice" Agriculture 10, no. 10: 428. https://doi.org/10.3390/agriculture10100428