Identification of a Plasma Microrna Signature as Biomarker of Subaneurysmal Aortic Dilation in Patients with High Cardiovascular Risk
Abstract
:1. Introduction
2. Experimental Section
2.1. Study Design and Population
2.2. Sample Collection
2.3. RNA Isolation
2.4. cDNA Synthesis and Identification of Plasma miR Expression Profiling
2.5. Validation of Plasma miR Expression Profiling by qRT-PCR Analysis
2.6. Target Gene Prediction and Functional Enrichment Analysis
2.7. Statistical Analysis
3. Results
3.1. Clinical Characteristics of Patients
3.2. Identification of Plasma miRs Differentially Expressed in Patients with SAD
3.3. Validation of miR Expression by Individual qRT-PCR
3.4. Plasma miRs as Predictors of SAD in Patients with High Cardiovascular Risk
3.5. Correlations of miRs with Clinical Parameters
3.6. Target Gene Prediction and Functional Enrichment Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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All | CT | SAD | AAA | |
---|---|---|---|---|
(n = 300) | (n = 231) | (n = 47) | (n = 22) | |
Age, years | 67 ± 3 | 67 ± 3 | 66 ± 5 | 75 ± 3 * |
Male, n (%) | 162 (54.0) | 105 (45.5) | 40 (85.1) * | 17 (77.3) * |
BMI, kg/m2 | 28.3 ± 1.1 | 28.4 ± 1.0 | 28.7 ± 1.7 | 27.5 ± 2.3 |
WC, cm | 98 ± 3 | 97 ± 3 | 101 ± 4 | 100 ± 4 |
Smoking habits, n (%) | 131 (35.7) | 85 (36.8) | 31 (66.0) * | 15 (68.2) * |
Alcohol, n (%) | 51 (17.1) | 29 (12.6) | 16 (34.0) * | 6 (27.3) * |
Hypertension, n (%) | 300 (100) | 231 (100) | 47 (100) | 22 (100) |
SBP (mmHg) | 140 ± 3 | 141 ± 3 | 140 ± 4 | 132 ± 7 |
DBP (mmHg) | 80 ± 5 | 80 ± 3 | 80 ± 5 | 75 ± 4 |
Dyslipidemia, n (%) | 178 (59.3) | 134 (58.0) | 31 (66.0) | 13 (59.1) |
Triglycerides, mg/dL | 96 (74–134) | 98 (74–129) | 110 (71–156) | 102 (80–142) |
TC, mg/dL | 188 ± 8 | 189 ± 8 | 182 ± 11 | 163 ± 13 * |
LDL-c, mg/dL | 111 ± 7 | 111 ± 7 | 111 ± 10 | 92 ± 14 |
HDL-c, mg/dL | 54 ± 3 | 52 ± 3 | 53 ± 5 | 47 ± 6 * |
T2DM, n (%) | 66 (22.0) | 50 (21.6) | 11 (23.4) | 5 (22.7) |
Glucose, mg/dL | 99 (90–111) | 99 (89–111) | 98 (94–116) | 99 (90–119) |
CHD, n (%) | 19 (6.3) | 9 (3.9) | 6 (12.8) * | 4 (18.2) * |
CVD, n (%) | 23 (7.7) | 16 (6.9) | 6 (12.8) * | 1 (4.5) |
PAD, n (%) | 12 (4.0) | 6 (2.6) | 2 (4.3) * | 4 (18.2) * |
eGFR, ml/min/1.72 m2 | 84.0 ± 4.5 | 84.5 ± 4.3 | 86.0 ± 7.3 | 70.5 ± 7.5 * |
AUC | 95% Confidence Interval | Specificity * | p-Value | |
---|---|---|---|---|
miR-339-3p † | 0.797 | 0.665–0.930 | 77.3 | <0.001 |
miR-28-3p | 0.741 | 0.599–0.884 | 54.5 | 0.004 |
miR-93-3p | 0.731 | 0.582–0.880 | 63.6 | 0.006 |
miR-378a-3p | 0.731 | 0.589–0.873 | 40.9 | 0.006 |
miR-150-5p | 0.729 | 0.583–0.875 | 45.5 | 0.007 |
miR-338-3p | 0.713 | 0.560–0.867 | 54.5 | 0.012 |
miR-29a-3p | 0.710 | 0.562–0.857 | 38.7 | 0.013 |
let-7b-5p | 0.698 | 0.546–0.849 | 45.5 | 0.019 |
miR-133b | 0.687 | 0.538–0.836 | 34.1 | 0.027 |
let-7e-5p | 0.677 | 0.524–0.829 | 31.8 | 0.037 |
miR-331-3p | 0.626 | 0.467–0.785 | 31.8 | 0.136 |
miR-27a-3p | 0.542 | 0.377–0.707 | 27.3 | 0.619 |
r | p-Value | |
---|---|---|
let-7b-5p | −0.172 | 0.184 |
let-7e-5p | −0.363 | 0.005 |
miR-27a-3p | −0.295 | 0.021 |
miR-28-3p | −0.392 | 0.002 |
miR-29a-3p | −0.519 | <0.001 |
miR-93-3p | −0.354 | 0.005 |
miR-133b | −0.311 | 0.017 |
miR-150-5p | −0.477 | <0.001 |
miR-331-3p | −0.282 | 0.030 |
miR-338-3p | −0.259 | 0.044 |
miR-339-3p | −0.345 | <0.001 |
miR-378a-3p | −0.349 | 0.007 |
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Torres-Do Rego, A.; Barrientos, M.; Ortega-Hernández, A.; Modrego, J.; Gómez-Gordo, R.; Álvarez-Sala, L.A.; Cachofeiro, V.; Gómez-Garre, D. Identification of a Plasma Microrna Signature as Biomarker of Subaneurysmal Aortic Dilation in Patients with High Cardiovascular Risk. J. Clin. Med. 2020, 9, 2783. https://doi.org/10.3390/jcm9092783
Torres-Do Rego A, Barrientos M, Ortega-Hernández A, Modrego J, Gómez-Gordo R, Álvarez-Sala LA, Cachofeiro V, Gómez-Garre D. Identification of a Plasma Microrna Signature as Biomarker of Subaneurysmal Aortic Dilation in Patients with High Cardiovascular Risk. Journal of Clinical Medicine. 2020; 9(9):2783. https://doi.org/10.3390/jcm9092783
Chicago/Turabian StyleTorres-Do Rego, Ana, María Barrientos, Adriana Ortega-Hernández, Javier Modrego, Rubén Gómez-Gordo, Luis A. Álvarez-Sala, Victoria Cachofeiro, and Dulcenombre Gómez-Garre. 2020. "Identification of a Plasma Microrna Signature as Biomarker of Subaneurysmal Aortic Dilation in Patients with High Cardiovascular Risk" Journal of Clinical Medicine 9, no. 9: 2783. https://doi.org/10.3390/jcm9092783