Next Article in Journal
Endometriosis and the Fallopian Tubes: Theories of Origin and Clinical Implications
Previous Article in Journal
Global Comparison of Changes in the Number of Test-Positive Cases and Deaths by Coronavirus Infection (COVID-19) in the World
Open AccessArticle

Small-Diameter Subchondral Drilling Improves DNA and Proteoglycan Content of the Cartilaginous Repair Tissue in a Large Animal Model of a Full-Thickness Chondral Defect

Center of Experimental Orthopaedics, Saarland University, Kirrberger Strasse 100, Building 37, D-66421 Homburg, Germany
*
Author to whom correspondence should be addressed.
Authors contributed equally to this work and shared first authorship.
J. Clin. Med. 2020, 9(6), 1903; https://doi.org/10.3390/jcm9061903
Received: 1 June 2020 / Accepted: 16 June 2020 / Published: 18 June 2020
(This article belongs to the Section Orthopedics)
This study quantified changes in the DNA content and extracellular matrix composition of both the cartilaginous repair tissue and the adjacent cartilage in a large animal model of a chondral defect treated by subchondral drilling. Content of DNA, proteoglycans, and Type II and Type I collagen, as well as their different ratios were assessed at 6 months in vivo after treatment of full-thickness cartilage defects in the femoral trochlea of adult sheep with six subchondral drill holes, each of either 1.0 mm or 1.8 mm in diameter by biochemical analyses of the repair tissue and the adjacent cartilage and compared with the original cartilage. Only subchondral drilling which were 1.0 mm in diameter significantly increased both DNA and proteoglycan content of the repair tissue compared to the original cartilage. DNA content correlated with the proteoglycan and Type II collagen content within the repair tissue. Significantly higher amounts of Type I collagen within the repair tissue and significantly increased DNA, proteoglycan, and Type I collagen content in the adjacent cartilage were identified. These translational data support the use of small-diameter bone-cutting devices for marrow stimulation. Signs of early degeneration were present within the cartilaginous repair tissue and the adjacent cartilage. View Full-Text
Keywords: articular cartilage; marrow stimulation; subchondral drilling; early osteoarthritis; extracellular matrix; proteoglycan; Type I collagen; Type II collagen; DNA articular cartilage; marrow stimulation; subchondral drilling; early osteoarthritis; extracellular matrix; proteoglycan; Type I collagen; Type II collagen; DNA
Show Figures

Figure 1

MDPI and ACS Style

Orth, P.; Eldracher, M.; Cucchiarini, M.; Madry, H. Small-Diameter Subchondral Drilling Improves DNA and Proteoglycan Content of the Cartilaginous Repair Tissue in a Large Animal Model of a Full-Thickness Chondral Defect. J. Clin. Med. 2020, 9, 1903.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop