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Open AccessArticle

Improved Isolation and Culture of Urine-Derived Stem Cells (USCs) and Enhanced Production of Immune Cells from the USC-Derived Induced Pluripotent Stem Cells

1
Department of Stem Cell & Regenerative Biotechnology and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea
2
Department of Urology, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul 05029, Korea
*
Author to whom correspondence should be addressed.
Kyeongseok Kim and Minchan Gil contributed equally to this work.
J. Clin. Med. 2020, 9(3), 827; https://doi.org/10.3390/jcm9030827
Received: 6 February 2020 / Revised: 13 March 2020 / Accepted: 16 March 2020 / Published: 18 March 2020
(This article belongs to the Section Clinical Cytology)
The availability of autologous adult stem cells is one of the essential prerequisites for human stem cell therapy. Urine-derived stem cells (USCs) are considered as desirable cell sources for cell therapy because donor-specific USCs are easily and non-invasively obtained from urine. Efficient isolation, expansion, and differentiation methods of USCs are necessary to increase their availability. Here, we developed a method for efficient isolation and expansion of USCs using Matrigel, and the rho-associated protein kinase (ROCK) inhibitor, Y-27632. The prepared USCs showed significantly enhanced migration, colony forming capacity, and differentiation into osteogenic or chondrogenic lineage. The USCs were successfully reprogramed into induced pluripotent stem cells (USC-iPSCs) and further differentiated into kidney organoid and hematopoietic progenitor cells (HPCs). Using flavonoid molecules, the isolation efficiency of USCs and the production of HPCs from the USC-iPSCs was increased. Taken together, we present an improved isolation method of USCs utilizing Matrigel, a ROCK inhibitor and flavonoids, and enhanced differentiation of USC-iPSC to HPC by flavonoids. These novel findings could significantly enhance the use of USCs and USC-iPSCs for stem cell research and further application in regenerative stem cell-based therapies. View Full-Text
Keywords: urine stem cell; Y-27632; matrigel; 3,2′-DHF; 3,4′-DHF; hematopoietic stem cell; kidney organoid; cell isolation; hiPSC urine stem cell; Y-27632; matrigel; 3,2′-DHF; 3,4′-DHF; hematopoietic stem cell; kidney organoid; cell isolation; hiPSC
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Kim, K.; Gil, M.; Dayem, A.A.; Choi, S.; Kang, G.-H.; Yang, G.-M.; Cho, S.; Jeong, Y.; Kim, S.J.; Seok, J.; Kwak, H.J.; Kumar Saha, S.; Kim, A.; Cho, S.-G. Improved Isolation and Culture of Urine-Derived Stem Cells (USCs) and Enhanced Production of Immune Cells from the USC-Derived Induced Pluripotent Stem Cells. J. Clin. Med. 2020, 9, 827.

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