Next Article in Journal
Real-Time Estimation of the Risk of Death from Novel Coronavirus (COVID-19) Infection: Inference Using Exported Cases
Previous Article in Journal
Dysregulation of Calcium Handling in Duchenne Muscular Dystrophy-Associated Dilated Cardiomyopathy: Mechanisms and Experimental Therapeutic Strategies
Open AccessArticle

A Novel Proposal of Salivary Lymphocyte Detection and Phenotyping in Patients Affected by Sjogren’s Syndrome

Department of Therapeutic Dentistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia
V.A. Nasonova Research Institute of Rheumatology, 119991 Moscow, Russia
Department of Neuroscience, Reproductive and Odontostomatological Sciences, Oral Medicine Unit, Federico II University of Naples, 80131 Naples, Italy
Author to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(2), 521;
Received: 28 January 2020 / Revised: 10 February 2020 / Accepted: 12 February 2020 / Published: 14 February 2020
(This article belongs to the Section Stomatology)
A preliminary evaluation of the parotid secretion cellular composition in patients with Sjogren’s Syndrome (SS) and a diagnostic accuracy assessment of salivary lymphocyte detection and immunophenotyping in Sjogren’s Syndrome diagnosis and prognosis were performed. The study included 40 consecutive patients, aged 19–60 years, with parenchymal sialadenitis associated with Sjogren’s Syndrome, and 20 healthy donors. The exclusion criteria were exacerbation of sialadenitis, chronic infections, malignant neoplasms, and lymphoproliferative diseases. The standard diagnostic tests were minor salivary gland biopsy and parotid sialography. Immunophenotyping of parotid secretion lymphocytes was performed by multicolor flow cytometry. Lymphocytes were detectable in parotid secretion of patients affected by Sjogren’s Syndrome, both primary (pSS) and secondary (sSS) form, but not in that from healthy donors. Sensitivity, specificity, positive, and negative predictive values of lymphocytes detection in parotid saliva were 77.5%, 100%, 100%, and 69%, respectively. The mean numbers of the total T-cell population, T-helper cells, and T-cytotoxic cells were 71.7%, 41.6%, and 53%, respectively. The immunophenotype of lymphocytes obtained by patients’ parotid flow resembles the immunophenotypes of glandular biopsies currently known. Our preliminary data suggest the use of saliva as an alternative and non-invasive method for evaluating the prognosis of Sjogren’s Syndrome. View Full-Text
Keywords: salivary glands; saliva; lymphocytes; Sjogren’s syndrome; chronic sialadenitis salivary glands; saliva; lymphocytes; Sjogren’s syndrome; chronic sialadenitis
Show Figures

Figure 1

MDPI and ACS Style

Selifanova, E.; Beketova, T.; Spagnuolo, G.; Leuci, S.; Turkina, A. A Novel Proposal of Salivary Lymphocyte Detection and Phenotyping in Patients Affected by Sjogren’s Syndrome. J. Clin. Med. 2020, 9, 521.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop