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Open AccessArticle

Characterization of Non-Toxigenic Clostridioides difficile Strains Isolated from Preterm Neonates and In Vivo Study of Their Protective Effect

1
Faculty of Pharmacy, Paris University, INSERM UMR S-1139, 4 Avenue de l’Observatoire, 75006 Paris, France
2
National Reference Center for Clostridioides difficile, Saint-Antoine Hospital, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France
3
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 92290 Châtenay-Malabry, France
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(11), 3650; https://doi.org/10.3390/jcm9113650
Received: 20 October 2020 / Revised: 6 November 2020 / Accepted: 9 November 2020 / Published: 13 November 2020
(This article belongs to the Special Issue Clostridium difficile Infection: Unmet Needs and Unsolved Questions)
In a previous monocentric study in preterm neonates (PN), we described a high Clostridioides difficile colonization rate (74%) with two uncommon non-toxigenic strains (NTCD) belonging to PCR-ribotype (RT) (CE)847 and (CE)032. To determine the extent of carriage of both NTCD in other spatio-temporal settings, strains isolated in PN stools from two multicenter cohorts were characterized by PCR-ribotyping, MLVA and MLST. We also evaluated the protective role of two NTCD from these RT against C. difficile infection in a hamster caecitis model. Animals were administered either each NTCD alone (n = 7), or followed by a 027 strain (n = 9). A control group received only the 027 strain (n = 8). Clinical activity and colonization by C. difficile in stools were monitored daily until death or sacrifice at D20. We isolated 18 RT(CE)032 (ST-83) strains and 2 RT(CE)847 (ST-26) strains among 247 PN from both cohorts. Within each RT, strains were genetically related. The survival rate was significantly increased when animals received a RT(CE)847 or (CE)032 strain before the 027 strain (4/9 deaths, p = 0.029; 1/9 death, p = 0.0004, respectively). We describe two predominant uncommon NTCD strains, in a PN population from different healthcare facilities. Both NTCD provide a potential protection against C. difficile infection. View Full-Text
Keywords: Clostridioides difficile; nontoxigenic strains; preterm neonates; C. difficile infection; colonization; in vivo model Clostridioides difficile; nontoxigenic strains; preterm neonates; C. difficile infection; colonization; in vivo model
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MDPI and ACS Style

Couturier, J.; Franconeri, L.; Janoir, C.; Ferraris, L.; Syed-Zaidi, R.; Youssouf, A.; Gateau, C.; Hoys, S.; Aires, J.; Barbut, F. Characterization of Non-Toxigenic Clostridioides difficile Strains Isolated from Preterm Neonates and In Vivo Study of Their Protective Effect. J. Clin. Med. 2020, 9, 3650. https://doi.org/10.3390/jcm9113650

AMA Style

Couturier J, Franconeri L, Janoir C, Ferraris L, Syed-Zaidi R, Youssouf A, Gateau C, Hoys S, Aires J, Barbut F. Characterization of Non-Toxigenic Clostridioides difficile Strains Isolated from Preterm Neonates and In Vivo Study of Their Protective Effect. Journal of Clinical Medicine. 2020; 9(11):3650. https://doi.org/10.3390/jcm9113650

Chicago/Turabian Style

Couturier, Jeanne; Franconeri, Léa; Janoir, Claire; Ferraris, Laurent; Syed-Zaidi, Rabab; Youssouf, Anlyata; Gateau, Cécile; Hoys, Sandra; Aires, Julio; Barbut, Frédéric. 2020. "Characterization of Non-Toxigenic Clostridioides difficile Strains Isolated from Preterm Neonates and In Vivo Study of Their Protective Effect" J. Clin. Med. 9, no. 11: 3650. https://doi.org/10.3390/jcm9113650

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