Next Article in Journal
Is Routine Prophylaxis Against Pneumocystis jirovecii Needed in Liver Transplantation? A Retrospective Single-Centre Experience and Current Prophylaxis Strategies in Spain
Next Article in Special Issue
Comparison of the Efficacy and Safety of Trabeculectomy with Mitomycin C According to Concentration: A Prospective Randomized Clinical Trial
Previous Article in Journal
Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts
Previous Article in Special Issue
Retinal Vascular Features in Ocular Blunt Trauma by Optical Coherence Tomography Angiography
Due to scheduled maintenance work on our core network, there may be short service disruptions on this website between 16:00 and 16:30 CEST on September 25th.
Article

In Vivo Confocal Microscopy of the Corneal-Conjunctival Transition in the Evaluation of Epithelial Renewal after SLET

1
Ophthalmic Unit, Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Policlinico G.B. Rossi, P.le L.A. Scuro 10, 37134 Verona, Italy
2
Division of Ophthalmology, Department of Biomedical and Clinical Sciences, BKV, Linköping University, SE-581 83 Linköping, Sweden
3
Ophthalmic Unit, San Bassiano Hospital, Via dei Lotti, 40, 36061 Bassano del Grappa, Italy
4
The Veneto Eye Bank Foundation, Padiglione G. Rama, Via Paccagnella 11, 30174 Zelarino Venezia, Italy
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(11), 3574; https://doi.org/10.3390/jcm9113574
Received: 12 October 2020 / Revised: 26 October 2020 / Accepted: 4 November 2020 / Published: 6 November 2020
(This article belongs to the Special Issue Updates in Ocular Surgery)
Examination of the corneal surface by in vivo confocal microscopy (IVCM) allows for objective identification of corneal and conjunctival cell phenotypes to evaluate different epithelialization patterns. Detection of a corneal-conjunctival epithelial transition could be considered as a sign of restored epithelial function following simple limbal epithelial transplantation (SLET). This is a prospective, interventional case series. We assessed patients with limbal stem cell deficiency (LSCD) by IVCM, preoperatively and at monthly intervals following SLET. Sectors in the central and peripheral cornea were scanned. Immediately upon detection of multi-layered cells with the epithelial phenotype in the central cornea and confirmation of epithelial transition in all corneal sectors, the decision for keratoplasty was taken. Ten patients were enrolled. After SLET, epithelial phenotype in the central cornea and an epithelial transition were identified within six and nine months in seven and one patients, respectively. One patient was a partial success and one failed. Five patients underwent keratoplasty, with stable results up to 12 months. Identification of the epithelial transition zone by IVCM permits assessment of the efficacy of SLET, enabling subsequent planning of keratoplasty for visual rehabilitation. The stability of the corneal surface following keratoplasty confirms that the renewal of the corneal epithelium was effectively retained. View Full-Text
Keywords: in vivo confocal microscopy; simple limbal epithelial transplantation; corneal-conjunctival epithelial transition; keratoplasty; limbal stem cell deficiency in vivo confocal microscopy; simple limbal epithelial transplantation; corneal-conjunctival epithelial transition; keratoplasty; limbal stem cell deficiency
Show Figures

Figure 1

MDPI and ACS Style

Pedrotti, E.; Chierego, C.; Cozzini, T.; Merz, T.; Lagali, N.; De Gregorio, A.; Fasolo, A.; Bonacci, E.; Bonetto, J.; Marchini, G. In Vivo Confocal Microscopy of the Corneal-Conjunctival Transition in the Evaluation of Epithelial Renewal after SLET. J. Clin. Med. 2020, 9, 3574. https://doi.org/10.3390/jcm9113574

AMA Style

Pedrotti E, Chierego C, Cozzini T, Merz T, Lagali N, De Gregorio A, Fasolo A, Bonacci E, Bonetto J, Marchini G. In Vivo Confocal Microscopy of the Corneal-Conjunctival Transition in the Evaluation of Epithelial Renewal after SLET. Journal of Clinical Medicine. 2020; 9(11):3574. https://doi.org/10.3390/jcm9113574

Chicago/Turabian Style

Pedrotti, Emilio, Chiara Chierego, Tiziano Cozzini, Tommaso Merz, Neil Lagali, Alessandra De Gregorio, Adriano Fasolo, Erika Bonacci, Jacopo Bonetto, and Giorgio Marchini. 2020. "In Vivo Confocal Microscopy of the Corneal-Conjunctival Transition in the Evaluation of Epithelial Renewal after SLET" Journal of Clinical Medicine 9, no. 11: 3574. https://doi.org/10.3390/jcm9113574

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop