MRI-Tracking of Dental Pulp Stem Cells In Vitro and In Vivo Using Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles
Abstract
:1. Introduction
2. Subjects and Methods
2.1. Cell Isolation
2.2. Cell Culture
2.3. Cell Characterization
2.4. MTT Assay
2.5. Growth Kinetics
2.6. Cell Labeling
2.7. In Vitro Assessment by MRI
2.8. Prussian Blue Staining
2.9. Real-Time PCR
2.10. Flow Cytometry
2.11. In Vivo Assessment by MRI
2.12. Statistical Analysis
3. Results
3.1. Cell Characterization
3.2. MTT Assay
3.3. Prussian Blue Staining
3.4. Real-Time PCR
3.5. Flow Cytometry
3.6. In Vitro Assessment by MRI
3.7. In Vivo Assessment by MRI
4. Discussion
5. Conclusions
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Groups | Non-labeled DPSCs (h:min) | SPION-labeled DPSCs (h:min) | |
---|---|---|---|
Day | |||
Day 1 | 16:57 | 28:54 | |
Day 2 | 26:13 | 33:54 | |
Day 3 | 39:19 | 35:59 | |
Day 4 | 26:46 | 37:55 | |
Day 5 | 31:42 | 30:56 | |
Day 6 | 36:21 | 31:58 |
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Zare, S.; Mehrabani, D.; Jalli, R.; Saeedi Moghadam, M.; Manafi, N.; Mehrabani, G.; Jamhiri, I.; Ahadian, S. MRI-Tracking of Dental Pulp Stem Cells In Vitro and In Vivo Using Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles. J. Clin. Med. 2019, 8, 1418. https://doi.org/10.3390/jcm8091418
Zare S, Mehrabani D, Jalli R, Saeedi Moghadam M, Manafi N, Mehrabani G, Jamhiri I, Ahadian S. MRI-Tracking of Dental Pulp Stem Cells In Vitro and In Vivo Using Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles. Journal of Clinical Medicine. 2019; 8(9):1418. https://doi.org/10.3390/jcm8091418
Chicago/Turabian StyleZare, Shahrokh, Davood Mehrabani, Reza Jalli, Mahdi Saeedi Moghadam, Navid Manafi, Golshid Mehrabani, Iman Jamhiri, and Samad Ahadian. 2019. "MRI-Tracking of Dental Pulp Stem Cells In Vitro and In Vivo Using Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles" Journal of Clinical Medicine 8, no. 9: 1418. https://doi.org/10.3390/jcm8091418