Effect of Iron Supplementation on the Outcome of Non-Progressive Pulmonary Mycobacterium tuberculosis Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacteria and Chemicals
2.2. Experimental Design
2.3. Rabbit Infection and Treatment
2.4. Histology Staining
2.5. Measurement of Blood Parameters
2.6. Measurement of Plasma and Lung Tissue Iron
2.7. RNA Isolation from Rabbit Lung and Blood
2.8. Quantitative Real-Time PCR Analysis (qPCR)
2.9. Statistical Analysis
3. Results
3.1. Iron Parameters in Fe-Supplemented Rabbits during Acute or Chronic Mtb Infection
3.2. Systemic and Lung Fe Levels in Mtb-Infected Rabbits with or without Fe-Supplementation
3.3. Fe-Supplementation Does Not Affect Systemic Host Iron-Responsive Gene Expression during Mtb Infection
3.4. Fe-Supplementation Perturbs Expression of Host Iron-Responsive Genes in the Lungs of Mtb-Infected Rabbits
3.5. Fe-Supplementation Affects Systemic Expression of Host Immune Response Genes in Mtb-Infected Rabbits
3.6. Fe-Supplementation Perturbs Expression of Host Immune Response Genes in the Lungs of Mtb-Infected Rabbits
3.7. Fe-Supplementation Did Not Affect the Lung Bacillary Load in Mtb-Infected Rabbits
3.8. Fe-Supplementation Did Not Affect Disease Pathology in Rabbit Lungs Infected with Mtb
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Kolloli, A.; Singh, P.; Rodriguez, G.M.; Subbian, S. Effect of Iron Supplementation on the Outcome of Non-Progressive Pulmonary Mycobacterium tuberculosis Infection. J. Clin. Med. 2019, 8, 1155. https://doi.org/10.3390/jcm8081155
Kolloli A, Singh P, Rodriguez GM, Subbian S. Effect of Iron Supplementation on the Outcome of Non-Progressive Pulmonary Mycobacterium tuberculosis Infection. Journal of Clinical Medicine. 2019; 8(8):1155. https://doi.org/10.3390/jcm8081155
Chicago/Turabian StyleKolloli, Afsal, Pooja Singh, G. Marcela Rodriguez, and Selvakumar Subbian. 2019. "Effect of Iron Supplementation on the Outcome of Non-Progressive Pulmonary Mycobacterium tuberculosis Infection" Journal of Clinical Medicine 8, no. 8: 1155. https://doi.org/10.3390/jcm8081155