The Effect of Positive End-Expiratory Pressure on Lung Micromechanics Assessed by Synchrotron Radiation Computed Tomography in an Animal Model of ARDS
Abstract
:1. Introduction
2. Experimental Section
2.1. Experimental Setup
2.2. Synchrotron Radiation Computed Tomography
2.3. Study Protocol
2.4. Image Recording and Analysis
2.5. Data Analysis
2.6. Histological Study
2.7. Statistical Analysis
3. Results
3.1. Airspaces Number and Dimension in the Whole Lung Slice
3.2. Airspaces Number and Dimension in the Three ROIs
4. Discussion
4.1. Number and Dimensional Variation in Experimental ARDS
4.2. Technical Aspects and Limitations
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Variable | PEEP 12 | PEEP 9 | PEEP 6 | PEEP 3 | ZEEP |
---|---|---|---|---|---|
VT (mL) | 33.1 ± 17.6 | 28.6 ± 10.9 | 23.4 ± 13 | 17.3 ± 10.7 | 19.9 ± 9.0 |
Measured PEEP (cmH2O) | 12.4 ± 0.4 | 9.6 ± 0.6 | 6.8 ± 0.5 | 4.1 ± 0.7 | 1.1 ± 0.8 |
Ppeakrs (cmH2O) | 33.9 ± 5.7 | 32.7 ± 8.5 | 33.4 ± 11.1 | 34.1 ± 11.0 | 40.1 ± 7.7 |
Pplatrs (cmH2O) | 31.5 ± 6 | 29.9 ± 8.4 | 29.8 ± 9.8 | 31.7 ± 11.0 | 36.3 ± 7.3 |
Driving Pressure (cmH2O) | 21.4 ± 5.8 | 23.1 ± 8.6 | 26.6 ± 11.0 | 30.0 ± 10.9 | 39.0 ± 7.6 |
Crs (mL/cmH2O) | 1.6 ± 0.8 | 1.4 ± 0.8 | 1.1 ± 0.7 | 0.7 ± 0.6 | 0.5 ± 0.3 |
Parameters of Linear Regression | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
ROI | PEEP 12 | PEEP 9 | PEEP 6 | PEEP 3 | PEEP 0 | m | k | R2 | p | ||
Entire slice | ASdim (voxel) | ALL | 13.1 ± 2.2 | 11.1 ± 4.3 | 9 ± 5.7 | 8 ± 5.5 | 7.6 ± 4.3 | 0.55 | 6.03 | 0.21 | <0.01 (*) |
ASnum (n/mm3) | ALL | 188 ± 52.0 | 163.2 ± 86.3 | 145.2 ± 92.3 | 142.4 ± 104.9 | 146.4 ± 96.7 | 5.96 | 116.41 | 0.08 | 0.02 (*) | |
Regional analysis | ASdim (voxel) | SUB | 13.6 ± 1.8 | 13 ± 2.8 | 10.9 ± 4.1 | 9.9 ± 4.6 | 9.4 ± 3.6 | 0.41 | 8.54 | 0.22 | 0.02 (*) |
MAN | 12.9 ± 1.8 | 11.2 ± 3.5 | 9.1 ± 5.5 | 7.9 ± 5.4 | 7.4 ± 3.6 | 0.55 | 5.95 | 0.27 | <0.01 (*) | ||
COR | 12.9 ± 2.9 | 9.1 ± 5.9 | 6.9 ± 7.6 | 6.3 ± 6.9 | 5.9 ± 5.5 | 0.68 | 3.61 | 0.21 | 0.02 (*) | ||
ASnum (n/mm3) | SUB | 189.7 ± 56.7 | 174.9 ± 93.3 | 158.7 ± 98.6 | 160.4 ± 114.1 | 162.5 ± 109.8 | 4.85 | 136.21 | 0.05 | 0.28 | |
MAN | 193.0 ± 55.5 | 167.0 ± 90.9 | 152.3 ± 96.6 | 145.6 ± 109.7 | 149.7 ± 100.2 | 6.15 | 120.16 | 0.09 | 0.15 | ||
COR | 181.2 ± 55.5 | 144.7 ± 91.7 | 124.6 ± 99.8 | 121.3 ± 111.7 | 127.1 ± 99.1 | 6.89 | 92.86 | 0.10 | 0.12 |
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Scaramuzzo, G.; Broche, L.; Pellegrini, M.; Porra, L.; Derosa, S.; Tannoia, A.P.; Marzullo, A.; Borges, J.B.; Bayat, S.; Bravin, A.; et al. The Effect of Positive End-Expiratory Pressure on Lung Micromechanics Assessed by Synchrotron Radiation Computed Tomography in an Animal Model of ARDS. J. Clin. Med. 2019, 8, 1117. https://doi.org/10.3390/jcm8081117
Scaramuzzo G, Broche L, Pellegrini M, Porra L, Derosa S, Tannoia AP, Marzullo A, Borges JB, Bayat S, Bravin A, et al. The Effect of Positive End-Expiratory Pressure on Lung Micromechanics Assessed by Synchrotron Radiation Computed Tomography in an Animal Model of ARDS. Journal of Clinical Medicine. 2019; 8(8):1117. https://doi.org/10.3390/jcm8081117
Chicago/Turabian StyleScaramuzzo, Gaetano, Ludovic Broche, Mariangela Pellegrini, Liisa Porra, Savino Derosa, Angela Principia Tannoia, Andrea Marzullo, João Batista Borges, Sam Bayat, Alberto Bravin, and et al. 2019. "The Effect of Positive End-Expiratory Pressure on Lung Micromechanics Assessed by Synchrotron Radiation Computed Tomography in an Animal Model of ARDS" Journal of Clinical Medicine 8, no. 8: 1117. https://doi.org/10.3390/jcm8081117