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Article

A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network

by
J. Curtis Nickel
1,
Alisa J. Stephens-Shields
2,
J. Richard Landis
2,
Chris Mullins
3,
Adrie van Bokhoven
4,
M. Scott Lucia
4,
Jeffrey P. Henderson
5,
Bhaswati Sen
6,
Jaroslaw E. Krol
6,
Garth D. Ehrlich
6,7,* and
The MAPP Research Network
8,†
1
Department of Urology, Queen’s University, Kingston, ON K0H 2T0, Canada
2
Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
3
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
4
Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA
5
Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO 63110, USA
6
Departments of Microbiology & Immunology; Drexel University College of Medicine, Philadephia, PA 19102, USA
7
Department of Otolaryngology-Head and Neck Surgery, Drexel University College of Medicine, Philadelphia, PA 19102, USA
8
c/o Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
*
Author to whom correspondence should be addressed.
†
The MAPP Research Network members are listed in Appendix A.
J. Clin. Med. 2019, 8(3), 415; https://doi.org/10.3390/jcm8030415
Submission received: 5 February 2019 / Revised: 13 March 2019 / Accepted: 18 March 2019 / Published: 26 March 2019
(This article belongs to the Section Infectious Diseases)
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Abstract

We surveyed urine microbiota of females diagnosed with interstitial cystitis/bladder pain syndrome (IC/BPS) and matched control participants enrolled in the National Institutes of Health (NIH) Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network using the culture-independent methodology. Midstream urine specimens were analyzed with the Plex-ID molecular diagnostic platform that utilizes polymerase chain reaction–electrospray ionization–time-of-flight–mass spectrometry (PCR-ESI-TOF MS) to provide a comprehensive identification of bacterial and select fungal species. IC/BPS and control participants were evaluated for differences (presence, diversity, and abundance) in species and genus. Urine specimens obtained from 181 female IC/BPS and 182 female control participants detected a total of 92 species (41 genera). Mean (SD) species count was 2.49 (1.48) and 2.30 (1.28) among IC/BPS and control participants, respectively. Overall species composition did not significantly differ between IC/BPS and control participants at any level (p = 0.726 species level, p = 0.222 genus level). IC/BPS participants urine trended to an overabundance of Lactobacillus gasseri (p = 0.09) detected but had a lower prevalence of Corynebacterium compared with control participants (p = 0.002). The relative abundance data analysis mirrored the prevalence data differences with no significant differences in most species or genus abundance other than Lactobacillus gasseri and Corynebacterium (p = 0.08 and p = 0.001, respectively). No cause and/or effect conclusion can be drawn from this observation, but it suggests that a more comprehensive evaluation (vaginal, bowel, catheterized bladder and/or tissue-based specimens) of the lower urinary tract microbiota in IC/BPS patients is warranted.
Keywords: microbiota; microbiome; infection; interstitial cystitis; bladder pain syndrome microbiota; microbiome; infection; interstitial cystitis; bladder pain syndrome

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MDPI and ACS Style

Nickel, J.C.; Stephens-Shields, A.J.; Landis, J.R.; Mullins, C.; van Bokhoven, A.; Lucia, M.S.; Henderson, J.P.; Sen, B.; Krol, J.E.; Ehrlich, G.D.; et al. A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network. J. Clin. Med. 2019, 8, 415. https://doi.org/10.3390/jcm8030415

AMA Style

Nickel JC, Stephens-Shields AJ, Landis JR, Mullins C, van Bokhoven A, Lucia MS, Henderson JP, Sen B, Krol JE, Ehrlich GD, et al. A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network. Journal of Clinical Medicine. 2019; 8(3):415. https://doi.org/10.3390/jcm8030415

Chicago/Turabian Style

Nickel, J. Curtis, Alisa J. Stephens-Shields, J. Richard Landis, Chris Mullins, Adrie van Bokhoven, M. Scott Lucia, Jeffrey P. Henderson, Bhaswati Sen, Jaroslaw E. Krol, Garth D. Ehrlich, and et al. 2019. "A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network" Journal of Clinical Medicine 8, no. 3: 415. https://doi.org/10.3390/jcm8030415

APA Style

Nickel, J. C., Stephens-Shields, A. J., Landis, J. R., Mullins, C., van Bokhoven, A., Lucia, M. S., Henderson, J. P., Sen, B., Krol, J. E., Ehrlich, G. D., & The MAPP Research Network. (2019). A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network. Journal of Clinical Medicine, 8(3), 415. https://doi.org/10.3390/jcm8030415

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