Next Article in Journal
Incidence of Capillary Leak Syndrome as an Adverse Effect of Drugs in Cancer Patients: A Systematic Review and Meta-Analysis
Next Article in Special Issue
The Morphology, Structure, Mechanical Properties and Biocompatibility of Nanotubular Titania Coatings before and after Autoclaving Process
Previous Article in Journal
Data Independent Acquisition Mass Spectrometry Can Identify Circulating Proteins That Predict Future Weight Loss with a Diet and Exercise Programme
Previous Article in Special Issue
Candida sp. Infections in Patients with Diabetes Mellitus
Article

Inflammatory Cell Recruitment in Candida glabrata Biofilm Cell-Infected Mice Receiving Antifungal Chemotherapy

1
LIBRO – ‘Laboratório de Investigação em Biofilmes Rosário Oliveira’, Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal
2
Laboratory for Process Engineering Environment Biotechnology and Energy-Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal
3
Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal
4
Instituto de Biologia Molecular e Celular, Universidade de Porto, 4200-135 Porto, Portugal
5
Departamento de Imuno-Fisiologia e Farmacologia, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2019, 8(2), 142; https://doi.org/10.3390/jcm8020142
Received: 26 December 2018 / Revised: 12 January 2019 / Accepted: 20 January 2019 / Published: 26 January 2019
(This article belongs to the Special Issue Biomaterial-Related Infections)
(1) Background: Due to a high rate of antifungal resistance, Candida glabrata is one of the most prevalent Candida spp. linked to systemic candidiasis, which is particularly critical in catheterized patients. The goal of this work was to simulate a systemic infection exclusively derived from C. glabrata biofilm cells and to evaluate the effectiveness of the treatment of two echinocandins—caspofungin (Csf) and micafungin (Mcf). (2) Methods: CD1 mice were infected with 48 h-biofilm cells of C. glabrata and then treated with Csf or Mcf. After 72 h, the efficacy of each drug was evaluated to assess the organ fungal burden through colony forming units (CFU) counting. The immune cell recruitment into target organs was evaluated by flow cytometry or histopathology analysis. (3) Results: Fungal burden was found to be higher in the liver than in the kidneys. However, none of the drugs was effective in completely eradicating C. glabrata biofilm cells. At the evaluated time point, flow cytometry analysis showed a predominant mononuclear response in the spleen, which was also evident in the liver and kidneys of the infected mice, as observed by histopathology analysis. (4) Conclusions: Echinocandins do not have a significant impact on liver and kidney fungal burden, or recruited inflammatory infiltrate, when mice are intravenously (i.v.) infected with C. glabrata biofilm-grown cells. View Full-Text
Keywords: Candida glabrata; candidemia; echinocandins; resistance; biofilms; infection; micafungin; caspofungin; in vivo Candida glabrata; candidemia; echinocandins; resistance; biofilms; infection; micafungin; caspofungin; in vivo
Show Figures

Figure 1

MDPI and ACS Style

Rodrigues, C.F.; Correia, A.; Vilanova, M.; Henriques, M. Inflammatory Cell Recruitment in Candida glabrata Biofilm Cell-Infected Mice Receiving Antifungal Chemotherapy. J. Clin. Med. 2019, 8, 142. https://doi.org/10.3390/jcm8020142

AMA Style

Rodrigues CF, Correia A, Vilanova M, Henriques M. Inflammatory Cell Recruitment in Candida glabrata Biofilm Cell-Infected Mice Receiving Antifungal Chemotherapy. Journal of Clinical Medicine. 2019; 8(2):142. https://doi.org/10.3390/jcm8020142

Chicago/Turabian Style

Rodrigues, Célia F., Alexandra Correia, Manuel Vilanova, and Mariana Henriques. 2019. "Inflammatory Cell Recruitment in Candida glabrata Biofilm Cell-Infected Mice Receiving Antifungal Chemotherapy" Journal of Clinical Medicine 8, no. 2: 142. https://doi.org/10.3390/jcm8020142

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop