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J. Clin. Med. 2019, 8(1), 23;

Attenuating Spinal Cord Injury by Conditioned Medium from Bone Marrow Mesenchymal Stem Cells

Neural Regeneration Laboratory, Department of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital, Taipei 11217, Taiwan
Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien 97401, Taiwan
Department of Medicine, National Yang-Ming University, Taipei 11217, Taiwan
Institute of Pharmacology, National Yang-Ming University, Taipei 11217, Taiwan
Author to whom correspondence should be addressed.
Received: 18 November 2018 / Revised: 20 December 2018 / Accepted: 21 December 2018 / Published: 25 December 2018
(This article belongs to the Section Neuroscience)
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Spinal cord injury (SCI) is a devastating neurological condition and might even result in death. However, current treatments are not sufficient to repair such damage. Bone marrow mesenchymal stem cells (BM-MSC) are ideal transplantable cells which have been shown to modulate the injury cascade of SCI mostly through paracrine effects. The present study investigates whether systemic administration of conditioned medium from MSCs (MSCcm) has the potential to be efficacious as an alternative to cell-based therapy for SCI. In neuron-glial cultures, MSC coculture effectively promoted neuronal connection and reduced oxygen glucose deprivation-induced cell damage. The protection was elicited even if neuron-glial culture was used to expose MSCcm, suggesting the effects possibly from released fractions of MSC. In vivo, intravenous administration of MSCcm to SCI rats significantly improved behavioral recovery from spinal cord injury, and there were increased densities of axons in the lesion site of MSCcm-treated rats compared to SCI rats. At early days postinjury, MSCcm treatment upregulated the protein levels of Olig 2 and HSP70 and also increased autophage-related proteins in the injured spinal cords. Together, these findings suggest that MSCcm treatment promotes spinal cord repair and functional recovery, possibly via activation of autophagy and enhancement of survival-related proteins. View Full-Text
Keywords: stem cell; released factors; spinal cord injury; neuroprotection stem cell; released factors; spinal cord injury; neuroprotection

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Tsai, M.-J.; Liou, D.-Y.; Lin, Y.-R.; Weng, C.-F.; Huang, M.-C.; Huang, W.-C.; Tseng, F.-W.; Cheng, H. Attenuating Spinal Cord Injury by Conditioned Medium from Bone Marrow Mesenchymal Stem Cells. J. Clin. Med. 2019, 8, 23.

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