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10.3390/jcm12185864
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Article

Determination of the Optimal Wavelength of the Hemolysis Index Measurement

by
Akiyo Ishiguro
1,2,†,
Mitsuaki Nishioka
2,†,
Akihiro Morishige
2,
Mai Yoneshiro
2,
Kanae Shinkawa
2,
Aki Fujinaga
2,
Toshihiko Kobayashi
2,
Yutaka Suehiro
1,2 and
Takahiro Yamasaki
1,2,*
1
Department of Oncology and Laboratory Medicine, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan
2
Division of Laboratory, Yamaguchi University Hospital, Ube 755-8505, Japan
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
J. Clin. Med. 2023, 12(18), 5864; https://doi.org/10.3390/jcm12185864
Submission received: 2 August 2023 / Revised: 6 September 2023 / Accepted: 8 September 2023 / Published: 9 September 2023
(This article belongs to the Section Clinical Laboratory Medicine)
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Abstract

Many biochemical auto-analyzers have methods that measure the hemolysis index (HI) to quantitatively assess the degree of hemolysis. Past reports on HI are mostly in vitro studies. Therefore, we evaluated the optimal wavelength of HI measurement ex vivo using clinical samples. Four different wavelengths (410/451 nm: HI-1, 451/478 nm: HI-2, 545/596 nm: HI-3 and 571/596 nm: HI-4) were selected for HI measurement, and correlations were examined from the measurement results of 3890 clinical samples. Another set of 9446 clinical samples was used to examine the correlation of HI with lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and potassium (K). Strong correlations were found between HI-4 and HI-1 and between HI-4 and HI-3. HI-1 and HI-2 cannot correctly assess hemolysis for high bilirubin samples, and HI-3 cannot correctly assess hemolysis for high triglyceride samples. LDH, AST and K correlated positively with HI-4 in clinical samples. For every 1-unit increase in HI-4, LDH increased by 19.51 U/L, AST by 1.03 U/L and K by 0.061 mmol/L, comparable to reports of other studies. In clinical samples, HI-4 was less susceptible to bilirubin and chyle and reflected well the changes in LDH, AST and K caused by hemolysis. This suggested that the optimal wavelength for HI measurement is 571 nm.
Keywords: hemolysis index; optimal wavelength; bilirubin; chyle hemolysis index; optimal wavelength; bilirubin; chyle
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MDPI and ACS Style

Ishiguro, A.; Nishioka, M.; Morishige, A.; Yoneshiro, M.; Shinkawa, K.; Fujinaga, A.; Kobayashi, T.; Suehiro, Y.; Yamasaki, T. Determination of the Optimal Wavelength of the Hemolysis Index Measurement. J. Clin. Med. 2023, 12, 5864. https://doi.org/10.3390/jcm12185864

AMA Style

Ishiguro A, Nishioka M, Morishige A, Yoneshiro M, Shinkawa K, Fujinaga A, Kobayashi T, Suehiro Y, Yamasaki T. Determination of the Optimal Wavelength of the Hemolysis Index Measurement. Journal of Clinical Medicine. 2023; 12(18):5864. https://doi.org/10.3390/jcm12185864

Chicago/Turabian Style

Ishiguro, Akiyo, Mitsuaki Nishioka, Akihiro Morishige, Mai Yoneshiro, Kanae Shinkawa, Aki Fujinaga, Toshihiko Kobayashi, Yutaka Suehiro, and Takahiro Yamasaki. 2023. "Determination of the Optimal Wavelength of the Hemolysis Index Measurement" Journal of Clinical Medicine 12, no. 18: 5864. https://doi.org/10.3390/jcm12185864

APA Style

Ishiguro, A., Nishioka, M., Morishige, A., Yoneshiro, M., Shinkawa, K., Fujinaga, A., Kobayashi, T., Suehiro, Y., & Yamasaki, T. (2023). Determination of the Optimal Wavelength of the Hemolysis Index Measurement. Journal of Clinical Medicine, 12(18), 5864. https://doi.org/10.3390/jcm12185864

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