EHO-85: A Multifunctional Amorphous Hydrogel for Wound Healing Containing Olea europaea Leaf Extract: Effects on Wound Microenvironment and Preclinical Evaluation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Amorphous Hydrogel Containing Olea Europaea Leaf Extract (EHO-85)
2.2. Moistening Ability of EHO-85 Compared to Other Amorphous Hydrogels
2.3. Rat Wound Model for the Study of the Effect of EHO-85 on Lipid Peroxidation and pH in the Wound Bed
2.4. Evaluation of the Antioxidant (Scavenger) Capacity of Hydrogel Containing EHO-85 in the Wound Bed of the Rat Wound Model, Assessed As Lipid Peroxidation and Reduced/Oxidized Glutathione Ratio
2.5. Effect of EHO-85 on Wound pH in an Animal Model
2.6. Effect of EHO-85 on Wound Healing in a Murine Model of Impaired Wound Healing (BKS. Cg-m +/+ Leprdb)
2.7. Statistical Analysis
3. Results
3.1. Moistening Capacity of the EHO-85 Hydrogel Compared to Other Amorphous Hydrogels
3.2. Evaluation of the Antioxidant (Scavenger) Capacity of the EHO-85 Hydrogel in the Wound Bed of Experimental Animals, Assessed As Lipid Peroxidation and the GSH/GSSG Ratio
3.3. Effect of EHO-85 on Wound Bed pH in an Animal Model
3.4. Effect of EHO-85 Amorphous Hydrogel with Antioxidant Properties on Wound Healing in a Murine Model of Impaired Wound Healing (BKS. Cg-m +/+ Leprdb)
4. Discussion
4.1. Moist Wound Healing (MWH): Moisturizing Effect on Wound Healing
4.2. Oxidative Stress Reduction and Wound Healing
4.3. pH Regulation and Wound Healing
4.4. Wound Healing Promotion In Vivo
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fluid Affinity (Donation) (%) Loss in Gel Weight | Type |
---|---|
0–5 | A |
>5–10 | B |
>10–15 | C |
>15–20 | D |
>20–25 | E |
Hydrogel | (%) Average Decrease of Gel Weight | Type |
---|---|---|
Askina® Gel | 19.0 ± 2.0 | D |
Normlgel® | 13.0 ± 3.0 | C |
Purilon® Gel | 12.5 ± 0.3 | C |
Intrasite® Gel | 6.0 ± 2.0 | B |
Nu-Gel® | 9.0 ± 3.0 | B |
EHO-85 | 15.2 ± 0.9 | D |
µM (MDA + 4HDA)/mg Protein | |||
---|---|---|---|
Treatment | 48 h | 96 h | |
EHO-85 without 0.1% OELE | 0.0097 ± 0.0005 | 0.0134 ± 0.0008 | p < 0.001 |
EHO-85 hydrogel | 0.0052 ± 0.0003 | 0.0073 ± 0.0004 | p < 0.001 |
p < 0.001 | p < 0.001 |
GSH/GSSG | |||
---|---|---|---|
Treatment | 48 h | 96 h | |
EHO-85 without 0.1% OELE | 0.382 ± 0.093 | 0.743 ± 0.050 | p < 0.05 |
EHO-85 | 0.346 ± 0.034 | 1.195 ± 0.174 | p < 0.05 |
ns | p < 0.05 |
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Casado-Díaz, A.; La Torre, M.; Priego-Capote, F.; Verdú-Soriano, J.; Lázaro-Martínez, J.L.; Rodríguez-Mañas, L.; Berenguer Pérez, M.; Tunez, I. EHO-85: A Multifunctional Amorphous Hydrogel for Wound Healing Containing Olea europaea Leaf Extract: Effects on Wound Microenvironment and Preclinical Evaluation. J. Clin. Med. 2022, 11, 1229. https://doi.org/10.3390/jcm11051229
Casado-Díaz A, La Torre M, Priego-Capote F, Verdú-Soriano J, Lázaro-Martínez JL, Rodríguez-Mañas L, Berenguer Pérez M, Tunez I. EHO-85: A Multifunctional Amorphous Hydrogel for Wound Healing Containing Olea europaea Leaf Extract: Effects on Wound Microenvironment and Preclinical Evaluation. Journal of Clinical Medicine. 2022; 11(5):1229. https://doi.org/10.3390/jcm11051229
Chicago/Turabian StyleCasado-Díaz, Antonio, Manuel La Torre, Feliciano Priego-Capote, José Verdú-Soriano, José Luis Lázaro-Martínez, Leocadio Rodríguez-Mañas, Miriam Berenguer Pérez, and Isaac Tunez. 2022. "EHO-85: A Multifunctional Amorphous Hydrogel for Wound Healing Containing Olea europaea Leaf Extract: Effects on Wound Microenvironment and Preclinical Evaluation" Journal of Clinical Medicine 11, no. 5: 1229. https://doi.org/10.3390/jcm11051229