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Emerging Therapies in Immune Thrombocytopenia
Article

The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia

1
Hematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, Spain
2
Medicine Faculty, Autonomous University of Madrid, Arzobispo Morcillo 4, 28029 Madrid, Spain
*
Author to whom correspondence should be addressed.
A.R.-L. and M.T.Á.R. contributed equally to this work.
Academic Editor: Tamam Bakchoul
J. Clin. Med. 2021, 10(8), 1661; https://doi.org/10.3390/jcm10081661
Received: 8 February 2021 / Revised: 26 March 2021 / Accepted: 9 April 2021 / Published: 13 April 2021
(This article belongs to the Special Issue Diagnosis and Management of Thrombocytopenia)
Loss of sialic acid from the carbohydrate side chains of platelet glycoproteins can affect platelet clearance, a proposed mechanism involved in the etiopathogenesis of immune thrombocytopaenia (ITP). We aimed to assess whether changes in platelet glycosylation in patients with ITP affected platelet counts, function, and apoptosis. This observational, prospective, and transversal study included 82 patients with chronic primary ITP and 115 healthy controls. We measured platelet activation markers and assayed platelet glycosylation and caspase activity, analysing samples using flow cytometry. Platelets from patients with ITP with a platelet count <30 × 103/µL presented less sialic acid. Levels of α1,6-fucose (a glycan residue that can directly regulate antibody-dependent cellular cytotoxicity) and α-mannose (which can be recognised by mannose-binding-lectin and activate the complement pathway) were increased in the platelets from these patients. Platelet surface exposure of other glycoside residues due to sialic acid loss inversely correlated with platelet count and the ability to be activated. Moreover, loss of sialic acid induced the ingestion of platelets by human hepatome HepG2 cells. Changes in glycoside composition of glycoproteins on the platelets’ surface impaired their functional capacity and increased their apoptosis. These changes in platelet glycoside residues appeared to be related to ITP severity. View Full-Text
Keywords: immune thrombocytopaenia; platelet apoptosis; sialic acid; platelet activation markers; glycoside residues immune thrombocytopaenia; platelet apoptosis; sialic acid; platelet activation markers; glycoside residues
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MDPI and ACS Style

Ramírez-López, A.; Álvarez Román, M.T.; Monzón Manzano, E.; Acuña, P.; Arias-Salgado, E.G.; Martín Salces, M.; Rivas Pollmar, M.I.; Jiménez Yuste, V.; Justo Sanz, R.; García Barcenilla, S.; Cebanu, T.; González Zorrilla, E.; Butta, N.V. The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia. J. Clin. Med. 2021, 10, 1661. https://doi.org/10.3390/jcm10081661

AMA Style

Ramírez-López A, Álvarez Román MT, Monzón Manzano E, Acuña P, Arias-Salgado EG, Martín Salces M, Rivas Pollmar MI, Jiménez Yuste V, Justo Sanz R, García Barcenilla S, Cebanu T, González Zorrilla E, Butta NV. The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia. Journal of Clinical Medicine. 2021; 10(8):1661. https://doi.org/10.3390/jcm10081661

Chicago/Turabian Style

Ramírez-López, Andrés, María T. Álvarez Román, Elena Monzón Manzano, Paula Acuña, Elena G. Arias-Salgado, Mónica Martín Salces, María I. Rivas Pollmar, Víctor Jiménez Yuste, Raul Justo Sanz, Sara García Barcenilla, Tamara Cebanu, Elena González Zorrilla, and Nora V. Butta 2021. "The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia" Journal of Clinical Medicine 10, no. 8: 1661. https://doi.org/10.3390/jcm10081661

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