Next Article in Journal
Macrolide-Resistant and Macrolide-Sensitive Mycoplasma pneumoniae Pneumonia in Children Treated Using Early Corticosteroids
Previous Article in Journal
Special Issue: Diagnosis and Management of Addiction and Other Mental Disorders (Dual Disorders)
Previous Article in Special Issue
Loop-Mediated Isothermal Amplification in Schistosomiasis
Article

Application of a Genus-Specific LAMP Assay for Schistosome Species to Detect Schistosoma haematobium x Schistosoma bovis Hybrids

1
Infectious and Tropical Diseases Research Group (e-INTRO), Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Biomedical Research Institute of Salamanca, Faculty of Pharmacy, University of Salamanca, 37007 Salamanca, Spain
2
IHPE, Université Montpellier, CNRS, Ifremer, Université Perpignan Via Domitia, 66100 Perpignan, France
3
Swiss Tropical and Public Health Institute, P.O. Box CH-4002 Basel, Switzerland
4
Department of Public Health, University of Basel, P.O. Box CH-4003 Basel, Switzerland
5
Unité de Formation et de Recherche Sciences Pharmaceutiques et Biologiques, Université Félix Houphouët-Boigny, Abidjan BPV 34, Côte d’Ivoire
6
Parasitology Laboratory, Institute of Natural Resources and Agrobiology (IRNASA, CSIC), Cordel de Merinas 40-52, 37008 Salamanca, Spain
*
Authors to whom correspondence should be addressed.
Academic Editor: Roberta Ettari
J. Clin. Med. 2021, 10(6), 1308; https://doi.org/10.3390/jcm10061308
Received: 1 February 2021 / Revised: 8 March 2021 / Accepted: 18 March 2021 / Published: 22 March 2021
(This article belongs to the Special Issue Epidemiology, Immunology, and Control of Schistosomiasis)
Schistosomiasis is a disease of great medical and veterinary importance in tropical and subtropical regions caused by different species of parasitic flatworms of the genus Schistosoma. The emergence of natural hybrids of schistosomes indicate the risk of possible infection to humans and their zoonotic potential, specifically for Schistosoma haematobium and S. bovis. Hybrid schistosomes have the potential to replace existing species, generate new resistances, pathologies and extending host ranges. Hybrids may also confuse the serological, molecular and parasitological diagnosis. Currently, LAMP technology based on detection of nucleic acids is used for detection of many agents, including schistosomes. Here, we evaluate our previously developed species-specific LAMP assays for S. haematobium, S. mansoni, S. bovis and also the genus-specific LAMP for the simultaneous detection of several Schistosoma species against both DNA from pure and, for the first time, S. haematobium x S. bovis hybrids. Proper operation was evaluated with DNA from hybrid schistosomes and with human urine samples artificially contaminated with parasites’ DNA. LAMP was performed with and without prior DNA extraction. The genus-specific LAMP properly amplified pure Schistosoma species and different S. haematobium-S. bovis hybrids with different sensitivity. The Schistosoma spp.-LAMP method is potentially adaptable for field diagnosis and disease surveillance in schistosomiasis endemic areas where human infections by schistosome hybrids are increasingly common. View Full-Text
Keywords: LAMP; schistosomiasis; schistosome hybrids; Schistosoma haematobium; Schistosoma bovis; molecular diagnosis; species-specific LAMP; genus-specific LAMP LAMP; schistosomiasis; schistosome hybrids; Schistosoma haematobium; Schistosoma bovis; molecular diagnosis; species-specific LAMP; genus-specific LAMP
Show Figures

Figure 1

MDPI and ACS Style

Crego-Vicente, B.; Fernández-Soto, P.; Febrer-Sendra, B.; García-Bernalt Diego, J.; Boissier, J.; Angora, E.K.; Oleaga, A.; Muro, A. Application of a Genus-Specific LAMP Assay for Schistosome Species to Detect Schistosoma haematobium x Schistosoma bovis Hybrids. J. Clin. Med. 2021, 10, 1308. https://doi.org/10.3390/jcm10061308

AMA Style

Crego-Vicente B, Fernández-Soto P, Febrer-Sendra B, García-Bernalt Diego J, Boissier J, Angora EK, Oleaga A, Muro A. Application of a Genus-Specific LAMP Assay for Schistosome Species to Detect Schistosoma haematobium x Schistosoma bovis Hybrids. Journal of Clinical Medicine. 2021; 10(6):1308. https://doi.org/10.3390/jcm10061308

Chicago/Turabian Style

Crego-Vicente, Beatriz, Pedro Fernández-Soto, Begoña Febrer-Sendra, Juan García-Bernalt Diego, Jérôme Boissier, Etienne K. Angora, Ana Oleaga, and Antonio Muro. 2021. "Application of a Genus-Specific LAMP Assay for Schistosome Species to Detect Schistosoma haematobium x Schistosoma bovis Hybrids" Journal of Clinical Medicine 10, no. 6: 1308. https://doi.org/10.3390/jcm10061308

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop