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Article

Collagen XII Deficiency Increases the Risk of Anterior Cruciate Ligament Injury in Mice

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Department of Medicine for Orthopaedics and Motor Organs, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-Ku, Tokyo 113-8421, Japan
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Medical Technology Innovation Center, Juntendo University, 2-1-1 Hongo, Bunkyo-Ku, Tokyo 113-8421, Japan
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Graduate School of Health and Sports Science, Juntendo University, 1-1 Hiragagakuenndai, Inzai 270-1695, Japan
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Department of Laboratory Animal Science, Faculty of Veterinary Science, Okayama University of Science, 1-3 Ikoinooka, Imabari 794-8555, Japan
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Department of Veterinary Surgery, Faculty of Veterinary Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama 700-0005, Japan
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Institute for Dental Research and Oral Musculoskeletal Biology and Center for Biochemistry, Medical Faculty, University of Cologne, D-50931 Cologne, Germany
*
Authors to whom correspondence should be addressed.
Academic Editors: Yoshitomo Saita and Rocco Papalia
J. Clin. Med. 2021, 10(18), 4051; https://doi.org/10.3390/jcm10184051
Received: 7 August 2021 / Revised: 26 August 2021 / Accepted: 30 August 2021 / Published: 7 September 2021
(This article belongs to the Special Issue Strategies for the Prevention of Knee Osteoarthritis)
Anterior cruciate ligament (ACL) rupture is a common knee injury for athletes. Although surgical reconstruction is recommended for the treatment of ACL ruptures, 100% functional recovery is unlikely. Therefore, the discovery of risk factors for ACL ruptures may prevent injury. Several studies have reported an association between polymorphisms of the collagen XII gene COL12A1 and ACL rupture. Collagen XII is highly expressed in tendons and ligaments and regulates tissue structure and mechanical property. Therefore, we hypothesized that collagen XII deficiency may cause ACL injury. To elucidate the influence of collagen XII deficiency on ACL, we analyzed a mouse model deficient for Col12a1. Four- to 19-week-old male Col12a1-/- and wild-type control mice were used for gait analysis; histological and immunofluorescent analysis of collagen XII, and real-time RT-PCR evaluation of Col12a1 mRNA expression. The Col12a1-/- mice showed an abnormal gait with an approximately 2.7-fold increase in step angle, suggesting altered step alignment. Col12a1-/- mice displayed 20–60% ACL discontinuities, but 0% discontinuity in the posterior cruciate ligament. No discontinuities in knee ligaments were found in wild-type mice. Collagen XII mRNA expression in the ACL tended to decrease with aging. Our study demonstrates for the first time that collagen XII deficiency increases the risk of ACL injury. View Full-Text
Keywords: collagen XII; ACL injury; risk factor collagen XII; ACL injury; risk factor
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MDPI and ACS Style

Fukusato, S.; Nagao, M.; Fujihara, K.; Yoneda, T.; Arai, K.; Koch, M.; Kaneko, K.; Ishijima, M.; Izu, Y. Collagen XII Deficiency Increases the Risk of Anterior Cruciate Ligament Injury in Mice. J. Clin. Med. 2021, 10, 4051. https://doi.org/10.3390/jcm10184051

AMA Style

Fukusato S, Nagao M, Fujihara K, Yoneda T, Arai K, Koch M, Kaneko K, Ishijima M, Izu Y. Collagen XII Deficiency Increases the Risk of Anterior Cruciate Ligament Injury in Mice. Journal of Clinical Medicine. 2021; 10(18):4051. https://doi.org/10.3390/jcm10184051

Chicago/Turabian Style

Fukusato, Shin, Masashi Nagao, Kei Fujihara, Taiju Yoneda, Kiyotaka Arai, Manuel Koch, Kazuo Kaneko, Muneaki Ishijima, and Yayoi Izu. 2021. "Collagen XII Deficiency Increases the Risk of Anterior Cruciate Ligament Injury in Mice" Journal of Clinical Medicine 10, no. 18: 4051. https://doi.org/10.3390/jcm10184051

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