Next Article in Journal
Ionizing Radiation for Preparation and Functionalization of Membranes and Their Biomedical and Environmental Applications
Previous Article in Journal
Reference Electrodes with Polymer-Based Membranes—Comprehensive Performance Characteristics
Open AccessArticle

Insignificant Impact of Chemotactic Responses of Pseudomonas aeruginosa on the Bacterial Attachment to Organic Pre-Conditioned RO Membranes

1
Water Desalination and Reuse Center (WDRC), Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia
2
Department of Biotechnology, Faculty of Applied Sciences, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands
*
Author to whom correspondence should be addressed.
Membranes 2019, 9(12), 162; https://doi.org/10.3390/membranes9120162
Received: 27 October 2019 / Revised: 25 November 2019 / Accepted: 28 November 2019 / Published: 2 December 2019
We investigated the impact of conditioning compositions on the way bacteria move and adhere to reverse osmosis (RO) membranes that have been pre-conditioned by organic compounds. We used humic acid (HA), bovine serum albumin (BSA), and sodium alginate (SA) to simulate conditioning layers on the RO membranes. First, we investigated the chemotactic responses of Pseudomonas aeruginosa PAO1 to the organic substances and the impact of changes in physicochemical characteristics of pre-conditioned membranes on bacterial attachment. Second, we observed bacterial attachment under the presence or absence of nutrients or microbial metabolic activity. Results showed that there was no relationship between the chemotactic response of P. aeruginosa PAO1 and the organic substances, and the changes in hydrophobicity, surface free energy, and surface charge resulting from changing the composition of the conditioning layer did not seem to affect bacterial attachment, whereas changing the roughness of the conditioned membrane exponentially did (exponential correlation coefficient, R2 = 0.85). We found that the initial bacterial attachment on the membrane surface is influenced by (i) the nutrients in the feed solution and (ii) the microbial metabolic activity, whereas the chemotaxis response has a negligible impact. This study would help to establish a suitable strategy to manage bacterial attachment. View Full-Text
Keywords: conditioning film; chemotaxis; Pseudomonas aeruginosa PAO1; reverse osmosis membrane conditioning film; chemotaxis; Pseudomonas aeruginosa PAO1; reverse osmosis membrane
Show Figures

Figure 1

MDPI and ACS Style

Kim, L.H.; Vrouwenvelder, J.S. Insignificant Impact of Chemotactic Responses of Pseudomonas aeruginosa on the Bacterial Attachment to Organic Pre-Conditioned RO Membranes. Membranes 2019, 9, 162.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop