Strong Static Magnetic Fields Increase the Gel Signal in Partially Hydrated DPPC/DMPC Membranes
Abstract
:1. Introduction
2. Results
2.1. Membrane Out-of-Plane Structure
Lipid Composition | Relative Humidity | Magnetic Field Strength (T) | Spacing | Gel Phase Lipid Area (Å) | |||||
---|---|---|---|---|---|---|---|---|---|
% | Salt | 0 | 1 | 2.5 | 3.5 | (Å) | 0 T Field | 3.5 T Field | |
DMPC/DPPC (1:1) | 97 | KSO | x | – | – | x | 43.04 | 42.98 | |
75 | NaCl | x | – | – | x | 43.24 | 43.03 | ||
50 | Mg(NO) | x | – | – | x | 42.70 | 42.70 | ||
43 | KCO | x | x | x | x | 42.51 | 42.51 | ||
25 | CHCOOK | x | – | – | x | 42.23 | 42.27 | ||
10 | LiCl | x | x | 42.30 | 42.21 |
2.2. Membrane In-Plane Structure
97% | 75% | 50% | 43% | 25% | 10% | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Field (T) | ord | gel | fluid | ord | gel | fluid | ord | gel | fluid | ord | gel | fluid | ord | gel | fluid | ord | gel | fluid |
0 | 1 | 161 ± 11 | 170 ± 27 | 1 | 372 ± 15 | 86 ± 30 | 1 | 691 ± 9 | 33 ± 39 | 1 | 648 ± 7 | 0 | 1 | 1029 ± 14 | 0 | 1 | 921 ± 12 | 0 |
3 | 384 ± 8 | 89 ± 10 | 5 | 691 ± 7 | 0 | |||||||||||||
1 | 4 | 691 ± 8 | 0 | |||||||||||||||
2.5 | 3 | 680 ± 8 | 0 | |||||||||||||||
3.5 | 2 | 160 ± 10 | 179 ± 24 | 2 | 383 ± 8 | 88 ± 11 | 2 | 688 ± 9 | 43 ± 39 | 2 | 675 ± 8 | 0 | 2 | 1033 ± 14 | 0 | 2 | 911 ± 13 | 0 |
6 | 691 ± 8 | 0 |
3. Discussion
3.1. Magnetic Fields Have an Effect on Dehydrated Lipid Membranes
3.2. A Potential Mechanism for Lipid Tails to Interact with Magnetic Fields
4. Conclusions
5. Materials and Methods
5.1. Neutron Diffraction
5.2. Membrane Preparation
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Tang, J.; Alsop, R.J.; Schmalzl, K.; Epand, R.M.; Rheinstädter, M.C. Strong Static Magnetic Fields Increase the Gel Signal in Partially Hydrated DPPC/DMPC Membranes. Membranes 2015, 5, 532-552. https://doi.org/10.3390/membranes5040532
Tang J, Alsop RJ, Schmalzl K, Epand RM, Rheinstädter MC. Strong Static Magnetic Fields Increase the Gel Signal in Partially Hydrated DPPC/DMPC Membranes. Membranes. 2015; 5(4):532-552. https://doi.org/10.3390/membranes5040532
Chicago/Turabian StyleTang, Jennifer, Richard J. Alsop, Karin Schmalzl, Richard M. Epand, and Maikel C. Rheinstädter. 2015. "Strong Static Magnetic Fields Increase the Gel Signal in Partially Hydrated DPPC/DMPC Membranes" Membranes 5, no. 4: 532-552. https://doi.org/10.3390/membranes5040532