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Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes

1
Laboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, Japan
2
Toray Research Centre, Inc., 1111 Tebiro, Kamakura, Kanagawa 248-8555, Japan
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Author to whom correspondence should be addressed.
Membranes 2014, 4(4), 764-777; https://doi.org/10.3390/membranes4040764
Received: 25 August 2014 / Revised: 13 October 2014 / Accepted: 28 October 2014 / Published: 13 November 2014
We isolated a high-purity carp glycophorin from carp erythrocyte membranes and prepared the oligosaccharide fraction from glycophorin by β-elimination [1]. The oligosaccharide fraction was separated into two components (P-1 and P-2) using a Glyco-Pak DEAE column. These O-linked oligosaccharides (P-1 and P-2) were composed of glucose, galactose, fucose, N-acetylgalactosamine and N-glycolylneuraminic acid (NeuGc). The P-1 and P-2 contained one and two NeuGc residues, respectively, and the P-1 exhibited bacteriostatic activity [1]. Using NMR and GC-MS, we determined that the structure of the bacteriostatic P-1 was NeuGcα2→6 (Fucα1→4) (Glcα1→3) Galβ1→4GalNAc-ol. This O-linked oligosaccharide was unique for a vertebrate with respect to the hexosamine and hexose linkages and its non-chain structure. View Full-Text
Keywords: fish; red blood cell membranes; erythrocyte; glycophorin; oligosaccharide; sialic acid; N-glycolylneuraminic acid fish; red blood cell membranes; erythrocyte; glycophorin; oligosaccharide; sialic acid; N-glycolylneuraminic acid
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Aoki, T.; Chimura, K.; Sugiura, H.; Mizuno, Y. Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes. Membranes 2014, 4, 764-777.

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