Uncovering the Mechanisms of Intracellular Membrane Trafficking by Reconstituted Membrane Systems

Intracellular membrane trafficking that transports proteins, lipids, and other substances between organelles is crucial for maintaining cellular homeostasis and signal transduction. The imbalance of membrane trafficking leads to various diseases. It is challenging to uncover the mechanisms of the complicated and dynamic trafficking process at the cellular or animal levels. The applications of functional reconstituted membrane systems, which can mimic the intracellular membrane compartments in a clean and simplified pattern, tremendously facilitate our understanding of the membrane trafficking process. In this review, we summarize applications of the in vitro membrane models, including liposomes, nanodiscs, and single-vesicle platforms, in elucidating molecular mechanisms that govern vesicle fusion and non-vesicular lipid transport, the key steps of membrane trafficking. This review highlights how membrane reconstitution approaches contribute to illustrating the protein-mediated molecular choreography of cellular membranes.