Structural and Functional Characterization of the Newly Designed Antimicrobial Peptide Crabrolin21
Abstract
:1. Introduction
2. Materials and Methods
2.1. Antimicrobial Susceptibility Tests
2.2. Liposome Preparation
2.3. CD Experiments
2.4. Membrane-Perturbing Activity Experiments
2.5. Hemolytic Activity Assay
2.6. NMR Spectroscopy
3. Results
3.1. Antimicrobial Assays
3.2. Membrane-Perturbing Activity
3.3. Secondary Structure of the Peptide: CD Experiments
3.4. Peptide–Membrane Association
3.5. Toxicity Assay
3.6. NMR Spectroscopy
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Crabrolin WT a | Crabrolin Minus a | Crabrolin Plus a | Crabrolin21 | Buffer b | |
---|---|---|---|---|---|
Minimal Inhibitory Concentration (µM) | |||||
Gram-negative bacteria | |||||
Escherichia coli ATCC 25922 | 200 | >383 | 24 | 4 | n.i. |
Pseudomonas aeruginosa ATCC 27853 | >400 | >383 | > 383 | 16 | n.i. |
Proteus mirabilis CCUG 26767 | >400 | >383 | >383 | >257 | n.i. |
Salmonella enterica Typhimurium LT2 | 200 | >383 | 48 | 8 | n.i. |
Klebsiella pneumoniae ATCC 13883 | >400 | >383 | 48 | 4 | n.i. |
Gram-positive bacteria | |||||
Bacillus subtilis ATCC 6633 | 200 | >383 | 48 | 4 | n.i. |
Staphylococcus aureus ATCC 29213 | 200 | >383 | 190 | 4 | n.i. |
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Cantini, F.; Giannì, P.; Bobone, S.; Troiano, C.; van Ingen, H.; Massoud, R.; Perini, N.; Migliore, L.; Savarin, P.; Sanders, C.; et al. Structural and Functional Characterization of the Newly Designed Antimicrobial Peptide Crabrolin21. Membranes 2023, 13, 365. https://doi.org/10.3390/membranes13030365
Cantini F, Giannì P, Bobone S, Troiano C, van Ingen H, Massoud R, Perini N, Migliore L, Savarin P, Sanders C, et al. Structural and Functional Characterization of the Newly Designed Antimicrobial Peptide Crabrolin21. Membranes. 2023; 13(3):365. https://doi.org/10.3390/membranes13030365
Chicago/Turabian StyleCantini, Francesca, Paola Giannì, Sara Bobone, Cassandra Troiano, Hugo van Ingen, Renato Massoud, Nicoletta Perini, Luciana Migliore, Philippe Savarin, Charles Sanders, and et al. 2023. "Structural and Functional Characterization of the Newly Designed Antimicrobial Peptide Crabrolin21" Membranes 13, no. 3: 365. https://doi.org/10.3390/membranes13030365