Membrane Repairing Capability of Non-Small Cell Lung Cancer Cells Is Regulated by Drug Resistance and Epithelial-Mesenchymal-Transition
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Development of Drug Resistant Cell Lines
2.3. MTS Assay
2.4. Inducing Cell EMT
2.5. Membrane Puncturing
2.6. Measuring Membrane Tension
2.7. Immunofluorescent Staining
2.8. Real Time Quantitative Polymerase Chain Reaction (RT-qPCR)
3. Results
3.1. Fast Membrane Resealing Response Observed in NSCLC Cancer Cell Lines
3.2. Membrane Resealing Speed Correlates with Cancer Cells’ Drug Resistivity
3.3. Epithelial-Mesenchymal-Transition Leads to Faster Membrane Resealing of Cancer Cells
4. Discussion and Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Gene Name | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
---|---|---|
E-Cadherin | TGCTGATGCCCCCAATACCCCA | GTGATTTCCTGGCCCACGCCAA |
N-Cadherin | TGACTCCAACGGGGACTGCACA | AGCTCAAGGACCCAGCAGTGGA |
Vimentin | AACCAACGACAAAGCCCGCGTC | TTCCGGTTGGCAGCCTCAGAGA |
Annexin A2 | TCGGACACATCTGGTGACTTCC | CCTCTTCACTCCAGCGTCATAG |
Annexin A5 | GTGGCTCTGATGAAACCCTCTC | GGCTCTCAGTTCTTCAGGTGTC |
Annexin A6 | GACTGACGAAGACACAATCATCG | CAGAATCAGCCTTGCCAGGTCT |
Annexin A7 | CGGATTGTGGTCACTCGAAGTG | CGGTAATCTCCACTCGTGTCAC |
GAPDH | GTCTCCTCTGACTTCAACAGCG | ACCACCCTGTTGCTGTAGCCAA |
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Xia, X.; Yang, H.; Au, D.W.-Y.; Lai, S.P.-H.; Lin, Y.; Cho, W.C.-S. Membrane Repairing Capability of Non-Small Cell Lung Cancer Cells Is Regulated by Drug Resistance and Epithelial-Mesenchymal-Transition. Membranes 2022, 12, 428. https://doi.org/10.3390/membranes12040428
Xia X, Yang H, Au DW-Y, Lai SP-H, Lin Y, Cho WC-S. Membrane Repairing Capability of Non-Small Cell Lung Cancer Cells Is Regulated by Drug Resistance and Epithelial-Mesenchymal-Transition. Membranes. 2022; 12(4):428. https://doi.org/10.3390/membranes12040428
Chicago/Turabian StyleXia, Xingyu, Hanbo Yang, Dennis Wai-Yin Au, Syrus Pak-Hei Lai, Yuan Lin, and William Chi-Shing Cho. 2022. "Membrane Repairing Capability of Non-Small Cell Lung Cancer Cells Is Regulated by Drug Resistance and Epithelial-Mesenchymal-Transition" Membranes 12, no. 4: 428. https://doi.org/10.3390/membranes12040428