Next Article in Journal
Extracorporeal Support Prognostication—Time to Move the Goal Posts?
Previous Article in Journal
Anchoring Water Soluble Phosphotungstic Acid by Hybrid Fillers to Construct Three-Dimensional Proton Transport Networks
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Silicon Carbide-Gated Nanofluidic Membrane for Active Control of Electrokinetic Ionic Transport

1
Department of Electronics and Telecommunications, Polytechnic of Turin, 10129 Turin, Italy
2
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA
3
Department of Applied Science and Technology, Polytechnic of Turin, 10129 Turin, Italy
4
Department of Surgery, Houston Methodist Research Institute, Houston, TX 77030, USA
5
Department of Radiation Oncology, Houston Methodist Research Institute, Houston, TX 77030, USA
*
Author to whom correspondence should be addressed.
Membranes 2021, 11(7), 535; https://doi.org/10.3390/membranes11070535
Submission received: 19 June 2021 / Revised: 6 July 2021 / Accepted: 12 July 2021 / Published: 15 July 2021
(This article belongs to the Section Membrane Analysis and Characterization)

Abstract

:
Manipulation of ions and molecules by external control at the nanoscale is highly relevant to biomedical applications. We report a biocompatible electrode-embedded nanofluidic channel membrane designed for electrofluidic applications such as ionic field-effect transistors for implantable drug-delivery systems. Our nanofluidic membrane includes a polysilicon electrode electrically isolated by amorphous silicon carbide (a-SiC). The nanochannel gating performance was experimentally investigated based on the current-voltage (I-V) characteristics, leakage current, and power consumption in potassium chloride (KCl) electrolyte. We observed significant modulation of ionic diffusive transport of both positively and negatively charged ions under physical confinement of nanochannels, with low power consumption. To study the physical mechanism associated with the gating performance, we performed electrochemical impedance spectroscopy. The results showed that the flat band voltage and density of states were significantly low. In light of its remarkable performance in terms of ionic modulation and low power consumption, this new biocompatible nanofluidic membrane could lead to a new class of silicon implantable nanofluidic systems for tunable drug delivery and personalized medicine.

1. Introduction

Advances in biomedical engineering aiming at new solutions for personalized medicine have fostered new developments in fields ranging from interface electronics to biological systems [1]. In this context, nanofluidic devices have been increasingly used for control and manipulation of ionic species [2], energy conversion [3,4,5,6], ionic devices [7,8,9], biosensing [10], analysis and separation of biomolecules [11,12], and drug delivery [1,13,14]. Along with this, solid-state technological processes for very large-scale integration (VLSI) devices have enabled the fabrication of devices with high resolution and high-aspect-ratio features such as nanometric channel membranes [9,13,14,15,16,17]. Among these systems, nanofluidic structures such as nanopores [18,19,20], nanoslits [21,22,23], and nanochannels [24,25,26] exhibit electrokinetic phenomena, such as ion current rectification, ion depletion, and ion gating, through the manipulation of their permselectivity. Due to their molecular feature size, nanofluidic channels can offer precise control of and interaction with charged species, opening the possibility to develop new biomedical devices and therapeutic platforms for personalized medicine as well as chronotherapeutic regimens for chronic and dysfunctional diseases [27,28,29]. The active interaction of charged surfaces with aqueous media containing charged species such as ions [30,31], DNA [32,33], proteins [7], and nanoparticles [34] permits the implementation of analysis and processing for biosensors, molecular separation techniques, and drug-delivery systems [13,14,27,28,29]. This electrostatic interaction results in accumulation of ionic species at the surface of the nanofluidic channels, known as the electrical double layer (EDL). These ions, called counterions, exhibit an opposite charge with respect to the nanofluidic surface charge, and their organization forms an electrostatic shield. This phenomenon results in electrostatic repulsion of the ions exhibiting the opposite charge of the counterions, called coions. The presence of the EDL causes the electric potential to decay to its bulk value over the characteristic length known as the Debye length λ (~1–100 nm). Then, modifying the surface charge through an electrostatic external stimulus such as an electric field leads to alteration of the EDL thickness. This phenomenon is widely used in nanofluidic structures to control the motion of the charged species. At present, several devices for electrostatic control of ionic species have been developed, such as ionic transistors [7,8,17], nanofluidic membranes with applied external electrodes [16,35], and anodic aluminum oxide (AAO) membranes [36]. These applications successfully demonstrated variations in the ion permselectivity of the nanochannels through exclusion enrichment [37] and concentration polarization [38] by applying an electrostatic field [39]. However, the limited ionic flow offered by ionic transistors is not suitable for drug-delivery applications; their channel density should be increased to reach an appropriate release rate [17]. Additionally, AAO membranes present a gate control localized at one end of the nanofluidic channels and a dispersed pore size distribution of the nanochannel due to the fabrication process, which can negatively affect fine control of release rate modulation. Our previous devices successfully modulated the extension of the EDL through electrostatic gate control applied with an embedded electrode buried on one side of the nanochannel [17] or external electrodes positioned on both sides of the membrane [35]. Nevertheless, the one-side electrode structure could alter the EDL only on one side of the nanofluidic structure. For this reason, the extension of the EDL over the entire nanochannel required a high electric field. In contrast, in the case of external electrodes, the nonintegrated metallic structure could be subjected to mechanical issues, such as detachment from the application site, leading to a lack of gate control.
The biomedical application for implantable drug delivery poses another challenge. The materials used to fabricate nanofluidic devices are crucial to the biocompatibility of the devices. Several studies were performed on different materials that could be used in biomedical applications [40,41,42,43]. a-SiC demonstrated higher mechanical stability, chemical inertness, and no evidence of corrosion under physiological conditions compared to SiO2 [15] and silicon nitride (Si3N4) [44]. Consequently, it is a suitable material for long-term implantable applications.
This study investigates the performance of a new gated nanofluidic membrane for electrostatic control of charged species for implantable applications of drug-based devices. Based on our knowledge, we created a nanofluidic membrane with a doped polysilicon buried gate electrode coated with amorphous silicon carbide (a-SiC) [15,45]. Using photolithographic processes widely employed in bio-nanoelectromechanical (BioNEMS) [46] and metal-oxide-semiconductor field-effect transistor (MOSFET) technologies, we achieved a high-density nanochannel-packed membrane that features a gate electrode deposited all around the nanochannel surface. The gating performance was evaluated by applying an electrostatic potential (−3 V to 3 V) to the gated nanochannels. The I-V characteristics were measured in 10−5 M potassium chloride (KCl) aqueous solution to assess the ionic FET (IFET) permselectivity of the nanofluidic membrane. Then, we evaluated the performance at higher concentrations (10−4 M KCl), where the ionic Debye length is smaller than the nanochannel size, in light of future biomedical applications such as reservoir-based implants. Furthermore, the study enabled identification of an asymmetric leakage current while performing electrostatic modulation of the EDL as well as estimation of the power consumption. To further investigate the physics of the devices, we inspected the behavior of the gated electrode of the nanochannel by reproducing it on a blank device, and we performed electrochemical impedance spectroscopy (EIS). We fitted the EIS measurements using a small-signal linear time-invariant (LTI) circuit, and we identified the transport phenomena responsible for the leakage current. Additionally, we evaluated the flat band potential and the density of the interface states. These findings can help in the design of nanofluidic membranes for fine-tunable electrostatic control of the drug release rate for implantable drug-based devices. The outcome of this investigation may lead to a new generation of silicon nanofluidic devices for implantable biomedical applications using VLSI technology.

2. Materials and Methods

2.1. Nanofluidic Membrane Fabrication

As previously described elsewhere [15], the lithographic fabrication process was performed on a silicon-on-insulator (SOI) 4-inch (100)-oriented p-doped wafer with a device layer (15 ± 1 µm), a buried oxide layer (1 µm), and a handle wafer (400 µm, Ultrasil Corporation, Hayward, CA, USA). After a first wet thermal oxidation step to produce 600 nm of sacrificial oxide on the surface of the device layer, nanoslit templates with a 500-nm width and a 6-µm length were patterned by using a reactive ion etching (RIE) step via inductively coupled plasma (ICP) deep silicon etching. On the other side of the SOI wafer, the handle wafer was patterned with a high density of hexagonally arranged circular microchannels by ICP to provide mechanical stability. Then, the built-up polymer covering the nanochannels and microchannels was cleaned, and the buried thermal oxide layer was removed in a buffered oxide etchant (BOE) solution. Nanochannels were shrunk by growing a layer of SiO2 through wet thermal oxidation. To build the buried gate electrode, a low-pressure chemical vapor deposition (LPCVD) step allowed the deposition of 121 nm of phosphorus-doped polysilicon (poly-Si) over the surface of the nanochannels. A further plasma-enhanced chemical vapor deposition (PECVD) step coated all of the wafer with a 64-nm a-SiC dielectric layer. Two contact pads (1 × 1 mm2) were created by selective removal of a-SiC by fluorine-based RIE at the edge of the membrane to expose the doped poly-Si. One hundred twenty total membranes were diced into 6 × 6 mm2 pieces using a dicing saw. Each membrane presents 199 microchannels organized in a hexagonal spatial configuration. Each microchannel is connected to 1400 identical slit nanochannels arranged in 19 rows and 96 columns, and consequently, the membrane chip features 278,600 nanochannels.

2.2. Blank Fabrication

A planar gate electrode was reproduced on the same type of wafer by following the same photolithographic process. Briefly, a 4-inch p-doped SOI wafer underwent a wet thermal oxidation step, producing a layer of SiO2. Then, the gate electrode was obtained via LPCVD of 121 nm of phosphorus-doped poly-Si. The wafer was diced in half before proceeding to coat it with the dielectric. On half of the wafer, a PECVD step deposited an a-SiC dielectric coating, and on the other half of the wafer, a wet thermal oxidation step produced a SiO2 coating. The contact pads for the poly-Si were created by selective removal of the dielectric by fluorine-based RIE at the edge of the blanks. Each half wafer hosted sixty 6 × 6 mm2 blanks diced via a dicing saw.

2.3. Electrode Connection

One contact pad exposing the poly-Si was connected to an insulated high-temperature 36 AWG wire via conductive silver epoxy (H20E, Epoxy Technology, Billerica, MA, USA) and underwent a 1-h curing step at 150 °C. The conductive exposed connection was isolated with UV epoxy (OG116, Epoxy Technologies, Inc., Billerica, MA, USA) and cured via UV for 2 h.

2.4. Setup for Electrochemical Measurements

An illustration of the setup for electrochemical measurements is reported elsewhere [15]. Electrochemical measurements were performed in a poly (methyl methacrylate) (PMMA, McMaster Carr, Douglasville, GA, USA) fixture. The setup system was constituted by two reservoirs containing 2 mL of solution, each with two Ag/AgCl electrodes (counter electrode and reference electrode). The samples under investigation (membranes and blank) were secured between the two reservoirs by rubber silicone O-rings (Apple Rubber, Lancaster, NY, USA). The fixture was screwed via 4 SS316L M3 screws. All measurements were performed using a multichannel potentiostat (Ivium Technologies B.V., Eindhoven, The Netherlands).

2.5. Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), and Ellipsometry

To assess the nanofluidic membrane structure, we morphologically inspected nanochannels by obtaining cross-sections of the nanofluidic membrane via a gallium ion milling-focused ion beam (FIB, FEI Dual-Beam 235 FIB, FEI, Hillsboro, OR, USA). The size of the nanochannels was measured with SEM (Nova NanoSEM 230, FEI, Hillsboro, OR, USA).
To investigate the thickness of the deposited layer, we performed ellipsometry measurements using a J.A. Woollam M2000U ellipsometer (Lincoln, NE, USA) on a-SiC and SiO2 blanks. To assess the roughness of the surface, we performed topographic mapping by AFM (Bruker MultiMode Atomic Force Microscope, Billerica, MA, USA).

2.6. Conductance, Current-Voltage (I-V) Curves, and Leakage Current

Electrochemical characterization of the nanochannels was performed in a KCl solution. The conductance was measured in a 4-electrode configuration using different concentrations of KCl, ranging from 10−7 M to 101 M. A potential across the membrane, also called the transmembrane potential (VDS), was applied using one channel of the potentiostat (Ivium Multichannels). To monitor the transmembrane current IDS, a staircase of 250 mV steps was applied from −1 V to 1 V in a 4-electrode configuration, and each step was held for 30 s to overcome transient phenomena. The conductance was calculated by taking the last sample point of each step and averaging the measured values. We repeated the measurements per membrane three times, and the complete study was performed on three membranes. During the conductance measurements, a gate potential was not applied.
The I-V characteristics and leakage current were evaluated in 10−5 M KCl and 10−4 M KCl. A voltage across the membrane (VDS) and a voltage on the gate electrode of the membrane (VGS) were applied using two channels of the potentiostat (Ivium Multichannels) to monitor the transmembrane current IDS and the leakage current IGS in parallel. To monitor IDS, a staircase of 250 mV steps was applied from −1 V to 1 V in a 4-electrode configuration, and each step was held for 30 s to overcome transient phenomena. VGS was applied between the gate electrode and two Ag/AgCl electrodes in the source reservoir facing the nanochannel layer device in a 3-electrode configuration with a DC potential ranging from −3 V to 3 V at a 500 mV step. IDS and IGS were measured under different VDS and VGS nested voltage sweeps applied to the working and reference electrodes.

2.7. EIS

EIS measurements were performed using blank samples with two different dielectrics, a-SiC and SiO2. The blank was inserted in the previous fixture, for which only the reservoir with two Ag/AgCl electrodes was used. The pressing of the blank samples against the cell via the O-ring left 7.07 mm2 exposed to the solutions. The measurements were performed in a 3-electrode configuration in 10−4 M KCl. Impedance spectra were obtained using a 50 mV VPP amplitude AC signal over a frequency range from 10−2 Hz to 105 Hz and a DC potential scan ranging from −3 V to 3 V, with a step of 500 mV. Mott-Schottky analysis was carried out at a 105 Hz frequency to calculate the carrier concentrations, flat band potential, and density of interface states.

3. Results and Discussion

3.1. Nanofluidic Membrane

The final nanofluidic membrane was inspected via optical microscopy to assess the mechanical integrity and the average size of the nanochannels measured via nitrogen flow test [47] was estimated ~106 nm. The etching procedure and layer deposition uniformity were analyzed using FIB-SEM microscopy. Figure 1A displays the 6 mm × 6 mm × 0.4 mm nanofluidic membrane, highlighting the electrode pads at the top left and bottom right edges. The membrane features 199 vertically oriented microchannels arranged in a hexagonal fashion to optimize the membrane porosity, simultaneously preserving the structural stability. Figure 1B presents an SEM image of the nanochannels densely packed in 19 rows and 96 columns for each microchannel. The nanochannel cross-section is shown in Figure 1C, and a detailed nanochannel cross-section with different layers is presented in Figure 1D.
The image shows that the layers were deposited, achieving good thickness uniformity despite the high aspect ratio of the nanochannels. In comparison with our previously developed nanofluidic technologies [17,48,49,50], the nanofluidic membrane requires a simplified photolithographic process owing to the easy connection derived from the vertical orientation of both microchannels and nanochannels [16,50] and features a substantially higher nanochannel number [17,51].
Compared to other nanofluidic devices for gate control, such as AAO [36] membranes fabricated with the gate electrode only at one end of the nanochannels, our nanochannel membrane features an extended buried polysilicon gate electrode. The buried electrode represents a crucial aspect that allows fine electrostatic control by modulating the ionic conductance along the entire length of the nanochannels. Additionally, the streamlined structure that connects nanochannels and microchannels permits the development of a nanofluidic membrane that features 278,600 nanochannels, permitting a sustainable release rate in view of future biomedical applications for therapeutic administration [15].

3.2. Blanks

3.2.1. Ellipsometry and AFM

The thickness of the deposited layers of the blank samples was assessed via ellipsometric measurements. Measurements related to a-SiC are presented in Figure 2A. The polysilicon layer in both cases was measured to be ~121 nm ± 15 nm, the a-SiC layer was measured to be ~64 nm ± 10 nm, and the SiO2 was measured to be ~66 nm ± 13 nm. Morphological evaluation of the surface of the dielectric coating was performed via AFM investigation. Measurements were performed at three different spots and averaged. Figure 2B shows a 50 μm × 50 μm AFM topographic map for an a-SiC blank device. The surface roughness was 22.9 nm ± 4.7 nm for a-SiC and 14.5 nm ± 3.9 nm for SiO2. The higher surface roughness measured for a-SiC blank devices can be associated with the pump-down vacuum phase verifiable during the fabrication process. During PECVD, the debris lying on the surface of the chamber could be redistributed on the sample surface by turbulent flows generated during the process.

3.3. EDL Modulation: Theoretical Background and Operation Mode

At the nanoscale, many transport processes in liquid are dictated by the interaction of ions with charged surfaces. Material surfaces exhibit an electrical charge when in contact with an aqueous medium where charged species are dissolved. To neutralize the electrical charges present on the surface, counterions electrostatically accumulate at the material/solution interface. Their distribution forms a layer called the EDL. Considering the Gouy-Chapman-Stern (GCS) model, the EDL is formed by two layers of ions. The first layer, called the compact layer, is composed of solvent molecules and ions that are immobile and adsorbed on the material–solution interface. The second layer positioned over the first layer is called the diffuse layer and consists of a mobile layer carrying a net charge within the solution. The thickness of the EDL depends on the ionic strength of the aqueous solution and the surface charge. In a nanochannel where the EDL thickness can be comparable to at least one of its sizes, the exclusion enrichment effect [37,52,53] and concentration polarization [38] can be observed. These phenomena can actively alter the diffusional flow of charged species and modify the ionic conductance in the nanochannel. Thus, modification of the surface charges leads to a variation in the EDL thickness and, consequently, ionic conductance. Control of the surface charge of a nanochannel can be obtained through application of an electrostatic field to the nanochannel wall, as shown in Figure 3. This phenomenon can be called electrostatic gate modulation [54]. Owing to this phenomenon, the surface charges of the nanochannels can be customized, allowing fine-tunable control of the ionic transport over the EDL thickness. Figure 3B shows the altered EDL thickness as a consequence of the electrostatic control applied to the nanochannel. To investigate the features of our nanofluidic membrane, we measured the conductivity to determine the surface charge of the nanochannels, while the gate control performance and permselectivity variation can be assessed through I-V measurements.

3.4. Ionic Conductance at the Floating Gate

To electrically characterize the nanochannels, we first performed nanochannel conductance measurements by applying transmembrane potential VDS ranging from −1 V to 1 V with the gate electrode floating. Figure 4 shows the nanochannel conductance as a function of KCl concentration. The conductance characteristic presents two parts: the bulk conductance occurring when the nanochannel height (h) is larger than the Debye length (λ), with the ratio h/λ » 1; the surface-dominated conductance region occurring when h is smaller than λ, with h/λ ~ 1 or h/λ < 1. In the bulk conductance region, the conductivity is linearly dependent on the electrolyte concentration. In contrast, in the surface-dominated conductance region, the conductivity shows a plateau due to the enrichment of the counterions to compensate and neutralize the surface charge. For this reason, the conductance only depends on the surface charge.
The transition between the two regions occurs at 10−5 M, where the Debye length is ~96 nm. Considering the conductance of the nanochannels as the superimposition of the two contributions, the bulk conductance and the surface charge conductance, we can apply the following formula [21]:
G = 10 3 ( μ K + + μ C l ) c N A e w h l + 2 μ K + σ w l
where μ K + and μ C l   are the ionic mobilities of K and Cl, c is the molar concentration, N A is Avogadro’s number, and w, h, and l are the width, height, and length of the nanochannel, respectively. Using the width and length obtained from the morphological characterizations, we evaluated the height of the nanochannels. By utilizing the bulk conductance formula, we obtained an average nanochannel height of ~106 nm. The results are consistent with the obtained gas flow test measurements [47]. Using the surface-dominated conductance formula (1), we calculated the surface charge on the nanochannel walls to be 0.471 μCm−2 at pH 7.4. This result is consistent with other surface charge values for SiC [55] and our previous studies [15,45].
The native surface charge exhibited by the a-SiC is smaller than that exhibited by the widely used SiO2 at pH 7.4 (1–100 mCm−2) [7]. This aspect provides benefits when gate control is needed. The reason lies in the need to electrostatically control the reaction at the interface that acts as a charge buffer. The ability to regulate the protonation and deprotonation of reactive groups present at the dielectric/electrolyte interface is key to generating and controlling the charge distribution in the double layer [56].

3.5. I-V Characteristics and Leakage Current

The nanofluidic membrane device has two distinguishing features: (1) phosphorus-doped polysilicon with an excess of electron carriers as the gate electrode and (2) low surface charge density. The first feature allows control of the leakage currents and consequently the power dissipation of the devices. Regarding the second aspect, exhibiting a low surface charge density environment can help with high-efficiency gate modulation. In fact, small changes in gate potentials can be relatively significant in the case of very low surface charge [57,58].
To test the performance of the nanofluidic membrane, ionic current IDS vs. VDS curves were measured as a function of VGS within the voltage range of |VGS| < 3 V at a step of 0.5 V and |VDS| < 1 V at a step of 0.25 V. To evaluate the power consumption, we simultaneously measured the leakage current IGS vs. VGS flowing from the gate to source. To test the ability of the nanofluidic membrane to modify the permselectivity of the nanochannels, we performed measurements in 10−5 M KCl, the concentration at which the nanochannels present the transition between the surface-charge-governed regime and the geometry-governed regime. Here, the electrolyte exhibited a Debye length comparable to the nanochannel height of the nanofluidic membrane. Moreover, we evaluated the gating performance of the nanofluidic membrane using 10−4 M KCl, a concentration at which λ < h. The purpose of this investigation is to evaluate the capability of the n-doped polysilicon electrode to manipulate the extension of the EDL in the nanochannel under the condition of the conductance being dictated by the geometry.
Figure 5 shows a representation of the ionic flow when gate and transmembrane potentials are applied. The transmembrane potential VDS influences the direction of the ions in the nanochannels. The application of VDS < 0 V directs potassium ions K+ to the drain electrode and negative chloride ions Cl to the source electrode, whereas VDS > 0 V conveys K+ to the source and Cl to the drain. Some of the ions in the nanochannels are partially involved in the formation of the EDL depending on the polarization potential applied to the gate electrode and consequently the exposed surface charge. The negative charge of the nanochannel wall and the n-doping profile of the polysilicon strongly influence the ionic transport in the nanochannel since it exhibits an excess of negative carriers (i.e., electrons in the polysilicon) that attract a majority of positive ions to the nanochannel wall. As a consequence, the ionic transport in the nanochannels and thus the conductance and leakage currents are strongly influenced.
Upon application of polarization potential VGS, the charged ionic species are electrostatically attracted to the nanochannel wall and participate in forming the EDL. VGS < 0 V attracts positive K+ to the nanochannel wall, creating a positively charged EDL. As a consequence, the number of positive ions K+ in the nanochannel increases (Figure 5A,B), and negative ions Cl are repelled. In contrast, VGS > 0 V attracts Cl ions to the nanochannel wall, creating a negative EDL and repelling K+ (Figure 5C,D).
The experimentally measured IDS-VDS characteristics and leakage current IGS-VGS in 10−5 M KCl and 10−4 M KCl are presented in Figure 6. The nanochannel membrane shows a unipolar behavior with a shallow surface charge density due to the a-SiC layer and the gate electrode, the so-called all-around-gate structure. Figure 6A,C shows IDS for 10−5 M KCl and 10−4 M KCl, respectively. At higher KCl concentrations, we obtained higher ionic current values. When VGS = 0 V, the ionic current shows a linear ohmic relationship between IDS and VDS. The same ionic current is expected for both coions and counterions, independent of the drain and source electrode, because potassium and chloride have similar mobilities in a liquid (76.2 × 10−7 m2/sV for K+ and 79.1 × 10−7 m2/sV for Cl) [59]. Upon applying the gate voltage, the ionic conductance can vary based on the polarity of the ions in the bulk.
In solid-state electronics such as FET devices, the electronic current depends on the gate voltage and the doping of the device, and the nanofluidic membrane exhibits behavior comparable to that of a p-type junction FET (JFET), where upon application of a positive gate voltage, the electronic current decreases. Application of VGS > 0 V leads to reduction in the ionic conductance and IDS over the range |VDS| < 1 V. In contrast, application of VGS < 0 V leads to two different scenarios based on the polarity of VDS. For both concentrations 10−5 M and 10−4 M KCl, a negative transmembrane potential VDS < 0 V causes increases in the ionic current and conductance in the nanochannels, whereas a positive transmembrane potential VDS > 0 V generates decreases in the ionic current and conductance in the case of 10−5 M KCl and a slight effect on the transmembrane current IDS and conductance in the case of 10−4 M KCl.
To explain the ionic transmembrane current behavior, we can refer to conduction mechanisms in an aqueous solution. The recombination process between the high number of electrons in the polysilicon and the ions in the aqueous solution [60,61] could be responsible for the different responses of the nanochannels. Additionally, we need to consider that a-SiC can act as an amorphous n-doped semiconductor [62]. Regarding this last aspect, the polarization potential VGS can strongly influence the conduction mechanism in the dielectric a-SiC. Due to its nature, a-SiC favors recombination of electrons with positive ionic species. Realistically, in the case of VGS < 0 V, some of the positive charges electrostatically attracted to the nanochannel wall could be involved in the conduction mechanism, and consequently, the thickness of the EDL could be reduced since the species return to their electroneutral state [60,61]. Upon application of VDS < 0 V, many positive ions are attracted into the nanochannels, increasing IDS and the conductivity. In contrast, VDS > 0 V drives negative charges into the nanochannels that are repelled by the application of a negative polarization potential. In the case of VGS > 0 V, the negative ions Cl attracted to the nanochannel wall are negligibly involved in the conduction mechanism. Higher values of VGS induce the EDL to extend in the nanochannels, leading to reduced IDS and conductance. Considering the IDS in 10−4 M KCl for VDS = 1 V, VGS = 3 V causes IDS to decrease from 600 nA to 129 nA, corresponding to a promising reduction of 78.5% in the ionic transmembrane flow. We also noted that for values of VGS in the range of 1.5 V to 3 V, IDS exhibits a slight reduction. This phenomenon can be associated with the electron depletion region formed at the polysilicon-dielectric interface. In fact, for a positive polarization potential, the electrostatic attraction of negative ions to the dielectric interface is achieved by the lack of electrons at the polysilicon-dielectric interface. This lack produces an electron depletion region, where positive electronic carriers (called holes) are responsible for electrostatic control of the EDL. Since the polysilicon is doped with an excess of electrons, holes are numerically inferior in the depletion region. Therefore, the positive electrostatic charge that can be modulated in the depletion region is lower than the negative charge originating from negative VGS. As a consequence, the EDL cannot be exceedingly extended.
The n-doping of the polysilicon and the behavior as a negative amorphous semiconductor of the a-SiC are responsible for the asymmetric leakage currents presented in Figure 6B,D. In fact, these two aspects strongly influence the conduction mechanism upon voltage application, working in favor of the conduction mechanism for negative gate potential VGS and against it for positive gate potential VGS. Further details are presented in the next section.
Figure 7 shows the nanochannel conductance for 10−4 M KCl as the ratio of the conductance modulated by the application of VGS and the conductance at the floating gate at VDS = −1 V and VDS = 1 V. The graph indicates that for VDS = −1 V, the conductance is enhanced for VGS < 0 V and reduced for VGS > 0 V. In the case of VDS = 1 V, VGS < 0 V does not significantly influence the conductance in the nanochannels, while VGS > 0 V causes a significant reduction in the normalized conductance. Additionally, the conductance exhibited at VGS = 3 V is barely reduced compared to the conductance calculated at VGS = 1.5 V. This finding is in agreement with the previously described ionic transport phenomena occurring in the nanochannels.
Leakage currents allow the estimation of the power consumption (Pdiss) of the nanofluidic membrane. The worst working conditions are created when IGS exhibits higher values in the range of applied gate potential of −3 V to 3 V. Referring to 10−4 M KCl, higher values of IGS are shown for VGS = ± 3 V and IDS = 0 V. In these cases, the power consumption (Pdiss) results in Pdiss = 4.2 μW for VGS = −3 V and Pdiss = 1.74 μW for VGS = 3 V. By applying VDS, Pdiss for VGS = 3 V can be reduced by approximately 22%, and in the case of VGS = −3 V, the power consumption can be reduced by 87%. Compared with previous work, the power consumption was reduced by one order of magnitude [15]. This can be related to the fact that the ionic strength of the solution used for this measurement is 1.37 times lower than the ionic strength of the solution used for our previous study [15].
An advantageous aspect of having asymmetric leakage currents lies in reducing the power consumption. In fact, we meet the need to reduce the ionic conductance by modulating the EDL extension in the nanochannel with the application of VGS > 0 V. Under this condition, the considerable performance of the nanofluidic membrane is associated with low power consumption.
The investigation of the capability of the nanofluidic membrane to electrostatically modulate the conductance and ionic transport in the nanochannel geometry-dictated regime can lay the groundwork to use biocompatible nanofluidic membranes in reservoir-based implants. In such devices, the highly concentrated drug in the reservoir is released at a rate established by the geometrical dimensions of the nanochannels [27,28,29,50]. In view of future manipulation of charged drug molecules, evaluation of the electrostatic control of ionic species exhibiting a Debye length λ almost one-third of the nanochannel height can provide a demonstration of the performance of the nanofluidic membrane.

3.6. EIS

To further investigate the physics of the nanofluidic membrane, we focused on the gate electrode interface immersed in 10−4 M KCl aqueous solution. To do so, we reproduced the dielectric-polysilicon heterojunction on a blank device, and we performed EIS. We conducted the same investigation on blanks with SiO2, comparing the dielectric performances. Measurements were performed in a frequency range from 10−2 Hz to 105 Hz by applying D.C. polarization potentials to the gate electrode from −3 V to 3 V at a step of 0.5 V. These bias conditions hold the structure at an operating bias point such that the behavior of the structure is fairly linear over a small range of voltages around the bias point. First, we determined the transport properties associated with the heterojunction immersed in KCl and associated a small-signal LTI electric circuit and the transfer function. The transfer function represents an essential aspect of designing electronic control customized based on physical phenomena between solids and electrolytes. Then, we calculated the flat band potential Vfb by using the Mott-Schottky plot. Compared with the SiO2, the a-SiC exhibits a lower Vfb, which is favorable for easily manipulating the surface charge. Then, we calculated the density of states Dit. These are “defects” that can be responsible for a higher leakage current and can be associated with the chemical process used to fabricate the dielectric/polysilicon interface.

3.6.1. Band Diagram Theory

When immersed in an aqueous solution, the blanks and the solution form a system that is organized into three layers and two interfaces: the first layer corresponds to the semiconductor n-polysilicon, which represents the buried electrode, the second layer corresponds to the dielectric coating, a-SiC or SiO2, and the third layer corresponds to the aqueous solution, as shown in Figure 8A. This is very similar to the solid-state MOS capacitor device composed of a metal layer, a dielectric layer, and a polysilicon layer. Here, the metal layer is replaced by the conductive electrolyte solution KCl, and the gate voltage is applied to the polysilicon. To explain how the polarization potential influences the carriers in the polysilicon, we can refer to the band theory. Application of a polarization potential puts the blank into one of three states: accumulation, flat band condition, and inversion, as illustrated in the band diagram presented in Figure 8B. When no polarization potential is applied with VGS = 0 V, the Fermi level EF in the polysilicon is pinned close to the conduction band EC due to the nature of the phosphorus donors. Notably, there is no Fermi level in the solution in contact with the dielectric since there is no redox couple in it. Applying a negative polarization potential VGS < 0 V to the electrode causes electron accumulation at the dielectric-semiconductor interface. The band bending presents a downward curvature and a thin layer, typically a few angstroms, of high electron concentration. This phenomenon leads to positive ions accumulating at the dielectric-electrolyte interface. Applying a positive polarization bias (VGS > 0) to the gate electrode causes a region depleted of electrons called the depletion zone to be formed. Here, the majority of the carriers are holes, which are the native charges of the semiconductor, and the band bending presents an upward curvature. Mainly, in both cases, band bending occurs in the semiconductor, and a small part of it occurs at the double layer. Although the chemical potential in the dielectric and electrolyte cannot be well defined, the electrostatic potential drop can be determined [63]. For a capacitor, V = q/C, the potential drop across the EDL is smaller than the potential drop across the dielectric (CEDL > CSD). According to the theory of solid-state semiconductor devices such as MOS capacitors [64], in the accumulation region, the capacitance increases due to the high number of electrons. Meanwhile, in the depletion region, the lack of electrons causes the capacitance to decrease until its value becomes almost constant.

3.6.2. Schematic Circuit

The impedance spectra of the data as Nyquist plots measured on a-SiC blank device are reported in Figure S1. An equivalent LTI model for the buried electrodes was proposed and is shown in Figure 8C. The equivalent circuit includes a combination of resistances (R) and capacitors (C). For interpretation of the results, the fact that the resistance is a parameter strongly related to the carrier transport properties must be considered, and at the same time, the capacitance is linked to the carriers. The circuit is composed of the following elements: RM represents the resistance of the electrochemical solution 10−4 M KCl. The first parallel part is associated with the electrolyte-dielectric interface, and it is composed of CEDL, the capacitance associated with the EDL, and RCT, the charge transfer resistance associated with the charge transfer through the dielectric coating. The second parallel part is associated with the dielectric-semiconductor interface, and it is composed of CSD, the capacitance associated with the semiconductor interface state capacitance, and RSD, the resistance associated with the semiconductor interface state resistance.
We considered the nonideality of the interfaces, taking into account the nonconstant thickness of the deposited layers (polysilicon layer ~121 nm ± 15 nm, a-SiC layer ~64 nm ± 10 nm, and SiO2 layer ~66 nm ± 13 nm) and the experimental roughness (a-SiC layer ~22.9 nm ± 4.7 nm and SiO2 layer ~14.5 nm ± 3.9 nm) measured as reported in Section 3.2.1 due to the deposition process and chemical defects that can be present in both the semiconductor polysilicon and dielectric layers [65,66,67,68]. For this reason, we used a constant phase element to relate to the i-th capacitance via the relationship Z C P E i = 1 Q i ( j ω ) n i , where Qi is the constant associated with the constant phase element, and ni is the empirical exponent measuring the distortion from the ideal impedance components. The constant phase element can be considered a capacitance when the value of ni satisfies 0.5 < n < 1. Figure 9 shows the RCT, RSD, QEDL, and QSD for both 64 nm a-SiC and 64 nm SiO2. The resistance associated with the electrolyte solution RM was estimated from the Nyquist plot and was found to be ~100 Ω in all measurements. Values were obtained by fitting the measured data with the circuit model shown in Figure 8C. Compared to the case without application of a polarization potential, both dielectrics SiC and SiO2 exhibit the same trend. Negative values of polarization potential VGS < 0 V cause RCT and RSD to decrease, while QEDL and QSD increase. For positive polarization potential VGS > 0 V, the opposite trend for RCT, RSD, QEDL, and QSD can be observed. We associated this behavior with the accumulation or depletion of electrons at the dielectric-polysilicon interface, as previously illustrated. According to band diagram theory, as previously reported, in the accumulation state occurring for VGS < 0 V, the high number of electrons at the polysilicon-dielectric interface leads to a higher availability of electrons that can participate in the transfer process from the polysilicon to the aqueous solution through the dielectric, leading to a decrease in resistances associated with the interfaces (Figure 9A,B). At the same time, this phenomenon increases the capacitance at the polysilicon-dielectric interface and consequently increases the capacitance of the EDL at the dielectric-electrolyte interface (Figure 9C,D). Analogously, in the depletion state, electron carriers are depleted. This phenomenon leads to increases in the interface resistances and decreases in the capacitances associated with both interfaces, polysilicon-dielectric and dielectric-electrolyte, with respect to the case of no polarization potential applied.
Compared with SiO2 at VGS = 0 V, the a-SiC dielectric shows an RCT hundreds of times smaller. By applying a polarization potential, considering the entire range of −3 V to 3 V, the RCT for a-SiC maintains the trend, and it remains between tens and hundreds of times smaller than the RCT for SiO2. Analogously, the a-SiC RDS is tens of times smaller than that of SiO2. Regarding the constant phase elements associated with the two electrolyte/dielectric and dielectric/semiconductor interfaces, nEDL and nSD are in the range of 0.7 to 1. This fact confirms that the constant phase element acts as a capacitance. The QEDL values exhibited by a-SiC are 1.5 to 8 times larger than those for SiO2. Very similar, the QSD values of a-SiC are 0.8 to 3.1 times larger than those of SiO2. We associated these differences with the fact that SiO2 is an insulator, while a-SiC can be considered as an amorphous semiconductor material. In amorphous materials, there are free immobilized electrons in the disordered network [69]. Their presence provides stored charges that increase the capacitance associated with these materials.
The corresponding relaxation times for the two interfaces can be determined as τ E D L = ( Q E D L R C T ) n E D L and τ D S = ( Q D S R D S ) n D S . These quantities correspond to the amount of time needed for polysilicon and the dielectric to reach equilibrium. They are related to the charge transfer phenomena and to the recombination lifetime of the carriers. Specifically, for a-SiC, τ E D L is between 3 ms and 1.8 s, while in the case of SiO2, τ E D L is between 50 ms and 30 s, where the maximum values are measured at VGS = 0 V. These values are in agreement with the theoretical explanation of amorphous semiconductor and insulator materials [70,71]. Regarding the recombination lifetime τ D S , a-SiC exhibits values between 8.9 μs and 12 μs, while SiO2 exhibits values between 38 μs and 43 μs. The measured values are in agreement with other values measured for polycrystalline silicon [72].
The total impedance exhibited by the buried electrode immersed in the ionic solution associated with the fitting electrical circuit (Figure 8C) can be written as:
Z = R M + ( 1 R C T + 1 Z C P E E D L ) 1 + ( 1 R D S + 1 Z C P E D S ) 1
The real and imaginary parts of the impedance can be separated and written as:
R e [ Z ] = R M + R C T ( 1 + R C T Q E D L ω n E D L cos n E D L π 2 ) 1 + 2 R C T Q E D L ω n E D L cos n E D L π 2 + ( R C T Q E D L ω n E D L ) 2 + R S D ( 1 + R S D Q S D ω n S D cos n S D π 2 ) 1 + 2 R S D Q S D ω n S D cos n S D π 2 + ( R S D Q S D ω n S D ) 2
I m [ Z ] = R C T 2 Q E D L ω n E D L sin n E D L π 2 1 + 2 R C T Q E D L ω n E D L cos n E D L π 2 + ( R C T Q E D L ω n E D L ) 2 + R S D 2 Q S D ω n S D cos n S D π 2 1 + 2 R S D Q S D ω n S D cos n S D π 2 + ( R S D Q S D ω n S D ) 2

3.6.3. High Frequency

The flat band potential is determined from the polysilicon-dielectric interface, and it is associated with the change in the working state of the electrode. Electrical transport properties such as the current flow through the dielectric are associated with the charge “trap” sites at the polysilicon-dielectric interface. These sites are responsible for capturing electrons from polysilicon, generating an electron-depletion region in polysilicon and increasing the availability of electrons that can participate in the transfer processes with the electrolyte [64]. To understand the amount of charges available to participate in the conduction transfer process, we analyzed the resistance and capacitance at 105 Hz. The chosen frequency corresponds to a short time scale that prevents filling and unfilling of the surface states and, consequently, building up of the double-layer capacitance. For this reason, the circuitry can be simplified as a single capacitor and a single resistor in parallel [63,73]. The capacitance-voltage (C-V) (Figure 10A) and conductance-voltage (G-V) (Figure 10B) values were used to determine the flat band potential, the number of donors, and the interface trap density.
The capacitance, C, and the conductance, G, were evaluated using the relationships
G = R e [ Z ] R e [ Z ] 2 + I m [ Z ] 2
and
C = I m [ Z ] ω ( R e [ Z ] 2 + I m [ Z ] 2 )
at 105 Hz. Im[Z] and Re[Z] are the real and imaginary parts of the impedance Z, respectively. The conductance and capacitance results (Figure 10) confirm that in the accumulation state, the high number of carriers favor higher C and G values, while in the depletion state, the lack of carriers leads to lower C and G values.
To determine the flat band voltage Vfb and the number of donors ND, we used the Mott–Schottky plot presented in Figure 11A for the polarization potential bias window of −3 V to 3 V. According to Mott–Schottky theory, the capacitance and potential are related by the equation
1 C 2 = 2 ε ε 0 A 2 e N D ( V V f b k B T e )
where C is the interfacial capacitance, A is the area exposed to the electrolyte, ND is the number of donors or carrier concentration, V is the applied voltage, Vfb is the flat band potential, kB is the Boltzmann constant, T is the absolute temperature, and e is the electronic charge.
We evaluated the carrier concentration ND and Vfb by taking the horizontal intercept of the 1/C2 versus V plot [64]. We estimated Vfb ~−0.23 V for 64 nm a-SiC and Vfb ~−0.70 V for 64 nm SiO2. We calculated the number of donors to be ND ~2.8 × 1015 cm−3 for a-SiC and ND ~5.67.8 × 1014 cm−3 for SiO2. The flat band voltage Vfb indicates the potential needed to neutralize the surface potential at the dielectric/electrolyte interface. The Vfb of the a-SiC blank is lower than that estimated for SiO2. As a consequence, the surface charge exhibited by the a-SiC is lower than that exhibited by the SiO2, confirming the previous results of a low surface charge exhibited by a-SiC in Section 3.4. This result represents a positive aspect since a positive surface potential can be switched to a negative surface potential by applying a low potential to the polysilicon. Regarding the number of donors, the slight difference found between a-SiC and SiO2 could be due to the roughness of the surface area (reported in Section 3.2.1) exposed to the electrolyte.
The density of interface states, shown in Figure 11B, was extracted for a-SiC and SiO2 from the G-V [74] data in the depletion [63] state using the equation [75]
G ω = e ω τ 1 + ( ω τ ) 2 ,
where τ represents the recombination lifetime, evaluable as the product of the measured charge transfer resistance RCT and the dielectric/polysilicon capacitance CSD at the interface, τ = R C T C S D .
The density of states indicates the general distribution of the electronic states in terms of energy. It is helpful for understating the conductivity and electrical transport phenomena at the interfaces. The Dit calculated for a-SiC and that for SiO2 exhibit similar trends (Figure 11B), and the values are low compared with other previously conducted studies [76]. The reason lies in the low ionic strength of the electrolyte used.
To evaluate the charge involved in the electrical transport mechanism, we can use the formula [77]
Q i t = e 2 D i t V ,
where e is the electron charge and V is the applied potential. The interface charge density values in the depletion state range from −1.6 × 109 e cm−2 to −3.56 × 109 e cm−2. SiO2 shows Qit values slightly higher in the range of −2.18 × 109 e cm−2 to −3.28 × 109 e cm−2. Due to the direct relationship with the density of states previously evaluated, the charges involved in the transport phenomena are fewer because of the corresponding low availability of charged ionic species in the electrolytic solution.

4. Conclusions and Future Outlook

In this paper, we have investigated the ability of a nanofluidic membrane to electrostatically control charged ions in a monovalent aqueous KCl solution through the application of a potential to the embedded electrode. The innovative approach implies a uniformly distributed doped polysilicon electrode buried in membrane nanochannels coated with biocompatible a-SiC. Using BioNEMS manufacturing techniques, an implantable nanofluidic membrane for fine-tunable electrostatic control was fabricated. The DC gating behavior of the nanochannel membrane was initially investigated through the I-V characteristics using 10−5 M KCl, which exhibits λ comparable to the nanochannel height. Furthermore, gating performances were investigated in the geometry-dictated regime using 10−4 M KCl. Depending on the polarity of the polarization potential VGS, the formed EDL favors or hinders ionic transport in the nanochannels. Considering a concentration of 10-4 M KCl, at VDS = −1 V, the nanochannel conductance was enhanced by 1.5 times for VGS = −3 V and reduced to 0.8 times for VGS = 3 V with a low power consumption ranging from 1.74 μW to 4.2 μW. AC electrochemical properties were determined using EIS. By using band diagram theory and associating an LTI electrical circuit with the gated electrode, electrical transport phenomena at the dielectric-electrolyte interface were investigated.
This study demonstrated that the nanofluidic membrane can noticeably control ionic species exhibiting a λ that is one-third of the nanochannel height, thus in a geometry-dictated regime. The proven performances could be very useful in reservoir-based implantable devices, where the drug molecules could exhibit a λ smaller than the nanochannel dimensions due to the high concentration in the drug reservoirs. In this case, the molecules would be transported in the nanochannels in a geometry-dictated regime, as in our study. Additionally, the investigation of electrical transport phenomena at the dielectric-electrolyte interface demonstrated that phosphorus-doped polysilicon favors the leakage current for a negative polarization potential due to the accumulation of electrons at the polysilicon-dielectric interface. Further studies need to be conducted to investigate the behavior of the nanochannel membrane when using a p-doped semiconductor electrode. In this case, the acceptor mobility is typically lower in a p-type semiconductor than the donor mobility in the n-type semiconductor, which would reduce the leakage current.

5. Patents

Gated Nanofluidic Valve for Active and Passive Electrosteric Control of Molecular Transport and Methods of Fabrication, U.S. Provisional Pat. Ser. No. 62/961,437, filed 15 January 2020.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/membranes11070535/s1, Figure S1: Impedance measurements presented as Nyquist plots performed on the a-SiC blank device recorded at different VGS.

Author Contributions

Conceptualization, A.S., N.D.T., D.D. and A.G.; data curation, A.S. and N.D.T.; formal analysis, A.S.; funding acquisition, X.L. and A.G.; investigation, A.S. and Y.W.; methodology, A.S. and L.I.; project administration, A.G.; resources, S.G. and A.G.; software, A.S. and L.I.; supervision, G.C., D.D. and A.G.; Validation, G.C., P.M.R., D.D. and A.G.; visualization, A.S. and N.D.T.; writing–original draft, A.S. All authors have read and agreed to the published version of the manuscript.

Funding

Funding support was provided by NIH-NIGMS R01GM127558 (AG) and the Houston Methodist Research Institute (AG).

Data Availability Statement

The data presented in this study are available within the manuscript.

Acknowledgments

We thank Jianhua (James) Gu from the electron microscopy core of the Houston Methodist Research Institute.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Prakash, S.; Piruska, A.; Gatimu, E.; Bohn, P.; Sweedler, J.; Shannon, M. Nanofluidics: Systems and Applications. Sens. J. IEEE 2008, 8, 441–450. [Google Scholar] [CrossRef]
  2. Haywood, D.G.; Saha-Shah, A.; Baker, L.A.; Jacobson, S.C. Fundamental Studies of Nanofluidics: Nanopores, Nanochannels, and Nanopipets. Anal. Chem. 2015, 87, 172–187. [Google Scholar] [CrossRef] [Green Version]
  3. Yan, Y.; Sheng, Q.; Wang, C.; Xue, J.; Chang, H.-C. Energy Conversion Efficiency of Nanofluidic Batteries: Hydrodynamic Slip and Access Resistance. J. Phys. Chem. C 2013, 117, 8050–8061. [Google Scholar] [CrossRef]
  4. Daiguji, H.; Yang, P.; Szeri, A.J.; Majumdar, A. Electrochemomechanical Energy Conversion in Nanofluidic Channels. Nano Lett. 2004, 4, 2315–2321. [Google Scholar] [CrossRef]
  5. van der Heyden, F.H.J.; Bonthuis, D.J.; Stein, D.; Meyer, C.; Dekker, C. Electrokinetic Energy Conversion Efficiency in Nanofluidic Channels. Nano Lett. 2006, 6, 2232–2237. [Google Scholar] [CrossRef] [PubMed]
  6. van der Heyden, F.H.J.; Bonthuis, D.J.; Stein, D.; Meyer, C.; Dekker, C. Power Generation by Pressure-Driven Transport of Ions in Nanofluidic Channels. Nano Lett. 2007, 7, 1022–1025. [Google Scholar] [CrossRef] [PubMed]
  7. Karnik, R.; Fan, R.; Yue, M.; Li, D.; Yang, P.; Majumdar, A. Electrostatic Control of Ions and Molecules in Nanofluidic Transistors. Nano Lett. 2005, 5, 943–948. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  8. Karnik, R.; Castelino, K.; Majumdar, A. Field-Effect Control of Protein Transport in a Nanofluidic Transistor Circuit. Appl. Phys. Lett. 2006, 88, 123114. [Google Scholar] [CrossRef]
  9. Bruno, G.; Di Trani, N.; Hood, R.L.; Zabre, E.; Filgueira, C.S.; Canavese, G.; Jain, P.; Smith, Z.; Demarchi, D.; Hosali, S.; et al. Unexpected Behaviors in Molecular Transport through Size-Controlled Nanochannels down to the Ultra-Nanoscale. Nat. Commun. 2018, 9, 1682. [Google Scholar] [CrossRef] [Green Version]
  10. Howorka, S.; Siwy, Z. Nanopore Analytics: Sensing of Single Molecules. Chem. Soc. Rev. 2009, 38, 2360–2384. [Google Scholar] [CrossRef]
  11. Yeh, L.-H.; Zhang, M.; Joo, S.W.; Qian, S.; Hsu, J.-P. Controlling PH-Regulated Bionanoparticles Translocation through Nanopores with Polyelectrolyte Brushes. Anal. Chem. 2012, 84, 9615–9622. [Google Scholar] [CrossRef]
  12. Li, W.; Bell, N.A.W.; Hernández-Ainsa, S.; Thacker, V.V.; Thackray, A.M.; Bujdoso, R.; Keyser, U.F. Single Protein Molecule Detection by Glass Nanopores. ACS Nano 2013, 7, 4129–4134. [Google Scholar] [CrossRef] [PubMed]
  13. Pons-Faudoa, F.P.; Sizovs, A.; Shelton, K.A.; Momin, Z.; Niles, J.A.; Bushman, L.R.; Xu, J.; Chua, C.Y.X.; Nichols, J.E.; Demaria, S.; et al. Preventive Efficacy of a Tenofovir Alafenamide Fumarate Nanofluidic Implant in SHIV-Challenged Nonhuman Primates. Adv. Ther. 2021, 4, 2000163. [Google Scholar] [CrossRef] [PubMed]
  14. Liu, H.-C.; Viswanath, D.I.; Pesaresi, F.; Xu, Y.; Zhang, L.; Di Trani, N.; Paez-Mayorga, J.; Hernandez, N.; Wang, Y.; Erm, D.R.; et al. Potentiating Antitumor Efficacy through Radiation and Sustained Intratumoral Delivery of Anti-CD40 and Anti-PDL1. Int. J. Radiat. Oncol. Biol. Phys. 2021, 110, 492–506. [Google Scholar] [CrossRef] [PubMed]
  15. Di Trani, N.; Silvestri, A.; Sizovs, A.; Wang, Y.; Erm, D.R.; Demarchi, D.; Liu, X.; Grattoni, A. Electrostatically Gated Nanofluidic Membrane for Ultra-Low Power Controlled Drug Delivery. Lab. Chip 2020, 20, 1562–1576. [Google Scholar] [CrossRef]
  16. Trani, N.D.; Silvestri, A.; Bruno, G.; Geninatti, T.; Chua, C.Y.X.; Gilbert, A.; Rizzo, G.; Filgueira, C.S.; Demarchi, D.; Grattoni, A. Remotely Controlled Nanofluidic Implantable Platform for Tunable Drug Delivery. Lab. Chip 2019, 19, 2192–2204. [Google Scholar] [CrossRef] [PubMed]
  17. Bruno, G.; Canavese, G.; Liu, X.; Filgueira, C.S.; Sacco, A.; Demarchi, D.; Ferrari, M.; Grattoni, A. The Active Modulation of Drug Release by an Ionic Field Effect Transistor for an Ultra-Low Power Implantable Nanofluidic System. Nanoscale 2016, 8, 18718–18725. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  18. Dekker, C. Solid-State Nanopores. Nat. Nanotechnol. 2007, 2, 209–215. [Google Scholar] [CrossRef]
  19. Li, J.; Stein, D.; McMullan, C.; Branton, D.; Aziz, M.J.; Golovchenko, J.A. Ion-Beam Sculpting at Nanometre Length Scales. Nature 2001, 412, 166–169. [Google Scholar] [CrossRef] [Green Version]
  20. Storm, A.J.; Chen, J.H.; Ling, X.S.; Zandbergen, H.W.; Dekker, C. Fabrication of Solid-State Nanopores with Single-Nanometre Precision. Nat. Mater. 2003, 2, 537–540. [Google Scholar] [CrossRef]
  21. Schoch, R.B.; Renaud, P. Ion Transport through Nanoslits Dominated by the Effective Surface Charge. Appl. Phys. Lett. 2005, 86, 253111. [Google Scholar] [CrossRef]
  22. Jo, K.; Dhingra, D.M.; Odijk, T.; de Pablo, J.J.; Graham, M.D.; Runnheim, R.; Forrest, D.; Schwartz, D.C. A Single-Molecule Barcoding System Using Nanoslits for DNA Analysis. Proc. Natl. Acad. Sci. USA 2007, 104, 2673–2678. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  23. Odijk, T. Scaling Theory of DNA Confined in Nanochannels and Nanoslits. Phys. Rev. E 2008, 77, 060901. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  24. Tonucci, R.J.; Justus, B.L.; Campillo, A.J.; Ford, C.E. Nanochannel Array Glass. Science 1992, 258, 783–785. [Google Scholar] [CrossRef]
  25. Pu, Q.; Yun, J.; Temkin, H.; Liu, S. Ion-Enrichment and Ion-Depletion Effect of Nanochannel Structures. Nano Lett. 2004, 4, 1099–1103. [Google Scholar] [CrossRef]
  26. Boukany, P.E.; Morss, A.; Liao, W.; Henslee, B.; Jung, H.; Zhang, X.; Yu, B.; Wang, X.; Wu, Y.; Li, L.; et al. Nanochannel Electroporation Delivers Precise Amounts of Biomolecules into Living Cells. Nat. Nanotechnol. 2011, 6, 747–754. [Google Scholar] [CrossRef]
  27. Ballerini, A.; Chua, C.Y.X.; Rhudy, J.; Susnjar, A.; Trani, N.D.; Jain, P.R.; Laue, G.; Lubicka, D.; Shirazi-Fard, Y.; Ferrari, M.; et al. Counteracting Muscle Atrophy on Earth and in Space via Nanofluidics Delivery of Formoterol. Adv. Ther. 2020, 3, 2000014. [Google Scholar] [CrossRef]
  28. Pons-Faudoa, F.P.; Trani, N.D.; Sizovs, A.; Shelton, K.A.; Momin, Z.; Bushman, L.R.; Xu, J.; Lewis, D.E.; Demaria, S.; Hawkins, T.; et al. Viral Load Reduction in SHIV-Positive Nonhuman Primates via Long-Acting Subcutaneous Tenofovir Alafenamide Fumarate Release from a Nanofluidic Implant. Pharmaceutics 2020, 12, 981. [Google Scholar] [CrossRef]
  29. Ferrati, S.; Nicolov, E.; Bansal, S.; Zabre, E.; Geninatti, T.; Ziemys, A.; Hudson, L.; Ferrari, M.; Goodall, R.; Khera, M.; et al. Delivering Enhanced Testosterone Replacement Therapy through Nanochannels. Adv. Healthc. Mater. 2015, 4, 446–451. [Google Scholar] [CrossRef] [Green Version]
  30. Li, C.; Xiong, T.; Yu, P.; Fei, J.; Mao, L. Synaptic Iontronic Devices for Brain-Mimicking Functions: Fundamentals and Applications. ACS Appl. Bio Mater. 2021, 4, 71–84. [Google Scholar] [CrossRef]
  31. Nishizawa, M.; Menon, V.P.; Martin, C.R. Metal Nanotubule Membranes with Electrochemically Switchable Ion-Transport Selectivity. Science 1995, 268, 700–702. [Google Scholar] [CrossRef] [Green Version]
  32. Gracheva, M.E.; Leburton, J.-P. Simulation of Electrically Tunable Semiconductor Nanopores for Ion Current/Single Bio-Molecule Manipulation. J. Comput. Electron. 2008, 7, 6–9. [Google Scholar] [CrossRef]
  33. Gracheva, M.E.; Xiong, A.; Aksimentiev, A.; Schulten, K.; Timp, G.; Leburton, J.-P. Simulation of the Electric Response of DNA Translocation through a Semiconductor Nanopore–Capacitor. Nanotechnology 2006, 17, 622–633. [Google Scholar] [CrossRef] [Green Version]
  34. Burg, T.P.; Godin, M.; Knudsen, S.M.; Shen, W.; Carlson, G.; Foster, J.S.; Babcock, K.; Manalis, S.R. Weighing of Biomolecules, Single Cells and Single Nanoparticles in Fluid. Nature 2007, 446, 1066–1069. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Bruno, G.; Geninatti, T.; Hood, R.L.; Fine, D.; Scorrano, G.; Schmulen, J.; Hosali, S.; Ferrari, M.; Grattoni, A. Leveraging Electrokinetics for the Active Control of Dendritic Fullerene-1 Release across a Nanochannel Membrane. Nanoscale 2015, 7, 5240–5248. [Google Scholar] [CrossRef] [Green Version]
  36. Kim, S.; Ozalp, E.I.; Darwish, M.; Weldon, J.A. Electrically Gated Nanoporous Membranes for Smart Molecular Flow Control. Nanoscale 2018, 10, 20740–20747. [Google Scholar] [CrossRef]
  37. Plecis, A.; Schoch, R.B.; Renaud, P. Ionic Transport Phenomena in Nanofluidics: Experimental and Theoretical Study of the Exclusion-Enrichment Effect on a Chip. Nano Lett. 2005, 5, 1147–1155. [Google Scholar] [CrossRef] [PubMed]
  38. abu-Rjal, R.; Chinaryan, V.; Bazant, M.Z.; Rubinstein, I.; Zaltzman, B. Effect of Concentration Polarization on Permselectivity. Phys. Rev. E 2014, 89, 012302. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  39. Pal Singh, K.; Kumari, K.; Kumar, M. Field-Effect Control of Electrokinetic Ion Transport in a Nanofluidic Channel. J. Appl. Phys. 2011, 110, 084301. [Google Scholar] [CrossRef]
  40. Saddow, S.E. Silicon Carbide Biotechnology: A Biocompatible Semiconductor for Advanced Biomedical Devices and Applications; Elsevier: Amsterdam, The Netherlands, 2012; ISBN 978-0-12-385906-8. [Google Scholar]
  41. Oliveros, A.; Guiseppi-Elie, A.; Saddow, S.E. Silicon Carbide: A Versatile Material for Biosensor Applications. Biomed. Microdevices 2013, 15, 353–368. [Google Scholar] [CrossRef]
  42. Iliescu, C.; Poenar, D.P. PECVD Amorphous Silicon Carbide (α-SiC) Layers for MEMS Applications. In Physics and Technology of Silicon Carbide Devices; Hijikata, Y., Ed.; InTech: London, UK, 2012; ISBN 978-953-51-0917-4. [Google Scholar]
  43. Iliescu, C.; Chen, B.; Poenar, D.P.; Lee, Y.Y. PECVD Amorphous Silicon Carbide Membranes for Cell Culturing. Sens. Actuators B Chem. 2008, 129, 404–411. [Google Scholar] [CrossRef]
  44. Knaack, G.L.; McHail, D.G.; Borda, G.; Koo, B.; Peixoto, N.; Cogan, S.F.; Dumas, T.C.; Pancrazio, J.J. In Vivo Characterization of Amorphous Silicon Carbide as a Biomaterial for Chronic Neural Interfaces. Front. Neurosci. 2016, 10, 301. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  45. Di Trani, N.; Silvestri, A.; Wang, Y.; Demarchi, D.; Liu, X.; Grattoni, A. Silicon Nanofluidic Membrane for Electrostatic Control of Drugs and Analytes Elution. Pharmaceutics 2020, 12, 679. [Google Scholar] [CrossRef]
  46. Avram, M.; Avram, A.; Bragaru, A.; Chen, B.; Poenar, D.P.; Iliescu, C. Low Stress PECVD Amorphous Silicon Carbide for MEMS Applications. In Proceedings of the CAS 2010 Proceedings (International Semiconductor Conference), Sinaia, Romania, 11–13 October 2010; Volume 01, pp. 239–242. [Google Scholar]
  47. Scorrano, G.; Bruno, G.; Di Trani, N.; Ferrari, M.; Pimpinelli, A.; Grattoni, A. Gas Flow at the Ultra-Nanoscale: Universal Predictive Model and Validation in Nanochannels of Ångstrom-Level Resolution. ACS Appl. Mater. Interfaces 2018, 10, 32233–32238. [Google Scholar] [CrossRef] [PubMed]
  48. Ferrati, S.; Fine, D.; You, J.; De Rosa, E.; Hudson, L.; Zabre, E.; Hosali, S.; Zhang, L.; Hickman, C.; Sunder Bansal, S.; et al. Leveraging Nanochannels for Universal, Zero-Order Drug Delivery in Vivo. J. Control. Release Off. J. Control. Release Soc. 2013, 172, 1011–1019. [Google Scholar] [CrossRef]
  49. Di Trani, N.; Jain, P.; Chua, C.Y.X.; Ho, J.S.; Bruno, G.; Susnjar, A.; Pons-Faudoa, F.P.; Sizovs, A.; Hood, R.L.; Smith, Z.W.; et al. Nanofluidic Microsystem for Sustained Intraocular Delivery of Therapeutics. Nanomed. Nanotechnol. Biol. Med. 2019, 16, 1–9. [Google Scholar] [CrossRef]
  50. Fine, D.; Grattoni, A.; Hosali, S.; Ziemys, A.; Rosa, E.D.; Gill, J.; Medema, R.; Hudson, L.; Kojic, M.; Milosevic, M.; et al. A Robust Nanofluidic Membrane with Tunable Zero-Order Release for Implantable Dose Specific Drug Delivery. Lab. Chip 2010, 10, 3074–3083. [Google Scholar] [CrossRef]
  51. Fine, D.; Grattoni, A.; Zabre, E.; Hussein, F.; Ferrari, M.; Liu, X. A Low-Voltage Electrokinetic Nanochannel Drug Delivery System. Lab. Chip 2011, 11, 2526–2534. [Google Scholar] [CrossRef]
  52. Di Trani, N.; Pimpinelli, A.; Grattoni, A. Finite-Size Charged Species Diffusion and PH Change in Nanochannels. ACS Appl. Mater. Interfaces 2020, 12, 12246–12255. [Google Scholar] [CrossRef] [PubMed]
  53. Grattoni, A.; Fine, D.; Zabre, E.; Ziemys, A.; Gill, J.; Mackeyev, Y.; Cheney, M.A.; Danila, D.C.; Hosali, S.; Wilson, L.J.; et al. Gated and Near-Surface Diffusion of Charged Fullerenes in Nanochannels. ACS Nano 2011, 5, 9382–9391. [Google Scholar] [CrossRef]
  54. Jin, X.; Aluru, N.R. Gated Transport in Nanofluidic Devices. Microfluid. Nanofluidics 2011, 11, 297–306. [Google Scholar] [CrossRef]
  55. Grosjean, A.; Rezrazi, M.; Tachez, M. Study of the Surface Charge of Silicon Carbide (SIC) Particles for Electroless Composite Deposits: Nickel-SiC. Surf. Coat. Technol. 1997, 96, 300–304. [Google Scholar] [CrossRef]
  56. Jiang, Z.; Stein, D. Electrofluidic Gating of a Chemically Reactive Surface. Langmuir 2010, 26, 8161–8173. [Google Scholar] [CrossRef] [PubMed]
  57. Fan, R.; Yue, M.; Karnik, R.; Majumdar, A.; Yang, P. Polarity Switching and Transient Responses in Single Nanotube Nanofluidic Transistors. Phys. Rev. Lett. 2005, 95, 086607. [Google Scholar] [CrossRef]
  58. Fan, R.; Huh, S.; Yan, R.; Arnold, J.; Yang, P. Gated Proton Transport in Aligned Mesoporous Silica Films. Nat. Mater. 2008, 7, 303–307. [Google Scholar] [CrossRef]
  59. Schoch, R.B.; Han, J.; Renaud, P. Transport Phenomena in Nanofluidics. Rev. Mod. Phys. 2008, 80, 839–883. [Google Scholar] [CrossRef] [Green Version]
  60. Zhu, F.; Hu, J.; Matulionis, I.; Deutsch, T.; Gaillard, N.; Kunrath, A.; Miller, E.; Madan, A. Amorphous Silicon Carbide Photoelectrode for Hydrogen Production Directly from Water Using Sunlight. Philos. Mag. 2009, 89, 2723–2739. [Google Scholar] [CrossRef]
  61. Matulionis, I.; Zhu, F.; Hu, J.; Deutsch, T.; Kunrath, A.; Miller, E.; Marsen, B.; Madan, A. Development of a Corrosion-Resistant Amorphous Silicon Carbide Photoelectrode for Solar-to-Hydrogen Photovoltaic/Photoelectrochemical Devices. Proc. SPIE Int. Soc. Opt. Eng. 2008, 7044, 70440D. [Google Scholar] [CrossRef]
  62. Magafas, L.; Georgoulas, N.; Thanailakis, A. Electrical Properties of A-SiC/c-Si(p) Heterojunctions. Semicond. Sci. Technol. 1992, 7, 1363–1368. [Google Scholar] [CrossRef]
  63. Meng, A.C.; Tang, K.; Braun, M.R.; Zhang, L.; McIntyre, P.C. Electrochemical Impedance Spectroscopy for Quantitative Interface State Characterization of Planar and Nanostructured Semiconductor-Dielectric Interfaces. Nanotechnology 2017, 28, 415704. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  64. Nicollian, E.H.; Brews, J.R. MOS (Metal Oxide Semiconductor) Physics and Technology; Wiley–Blackwell: New York, NY, USA, 1982; ISBN 978-0-471-08500-3. [Google Scholar]
  65. Alexander, C.; Tribollet, B.; Orazem, M. Contribution of Surface Distributions to Constant-Phase-Element (CPE) Behavior: 1. Influence of Roughness. Electrochim. Acta 2015, 173. [Google Scholar] [CrossRef]
  66. Vieira, D.; Fernandes, P.R. Surface Roughness Influence on CPE Parameters in Electrolytic Cells. Int. J. Electrochem. Sci. 2016, 11, 7775–7784. [Google Scholar] [CrossRef]
  67. Córdoba-Torres, P.; Mesquita, T.; Devos, O.; Tribollet, B.; Roche, V.; Nogueira, R. On the Intrinsic Coupling between Constant-Phase Element Parameters α and Q in Electrochemical Impedance Spectroscopy. Electrochim. Acta 2012, 72, 172–178. [Google Scholar] [CrossRef]
  68. Córdoba-Torres, P.; Mesquita, T.; Nogueira, R. Relationship between the Origin of Constant-Phase Element Behavior in Electrochemical Impedance Spectroscopy and Electrode Surface Structure. J. Phys. Chem. C 2015, 119, 150218064838009. [Google Scholar] [CrossRef]
  69. Ovshinsky, S.R. The Role of Free Radicals in the Formation of Amorphous Thin Films. In Disordered Materials: Science and Technology; Adler, D., Schwartz, B.B., Silver, M., Eds.; Institute for Amorphous Studies Series; Springer: Boston, MA, USA, 1991; pp. 114–119. ISBN 978-1-4684-8745-9. [Google Scholar]
  70. Viščor, P.; Olsen, N.B. Frequency and Time Response in Relaxation Time Semiconductors. J. Non-Cryst. Solids 1987, 90, 25–28. [Google Scholar] [CrossRef]
  71. Málek, J.; Shánělová, J. Structural Relaxation in Amorphous Materials. In Properties and Applications of Amorphous Materials; Thorpe, M.F., Tichý, L., Eds.; NATO Science Series; Springer: Dordrecht, The Netherlands, 2001; pp. 35–44. ISBN 978-94-010-0914-0. [Google Scholar]
  72. Orton, J. Polycrystalline and Amorphous Semiconductors; Oxford University Press: Oxford, UK, 2008; ISBN 978-0-19-171297-5. [Google Scholar]
  73. Chen, H.; Yuan, Y.; Yu, B.; Ahn, J.; McIntyre, P.C.; Asbeck, P.M.; Rodwell, M.J.W.; Taur, Y. Interface-State Modeling of $\hboxAl_2\hboxO_3$ –InGaAs MOS from Depletion to Inversion. IEEE Trans. Electron Devices 2012, 59, 2383–2389. [Google Scholar] [CrossRef]
  74. Nicollian, E.H.; Goetzberger, A. The Si-SiO2 Interface—Electrical Properties as Determined by the Metal-Insulator-Silicon Conductance Technique. Bell Syst. Tech. J. 1967, 46, 1055–1133. [Google Scholar] [CrossRef]
  75. Schroder, D.K. Semiconductor Material and Device Characterization; John Wiley & Sons: Hoboken, NJ, USA, 2015; ISBN 978-0-471-73906-7. [Google Scholar]
  76. Juan, H.L. Structural and Electrical Characterisations of Amorphous Silicon Carbide Films. Master’s Thesis, National University of Singapore, Singapore, 17 January 2005. [Google Scholar]
  77. Munakata, C.; Nishimatsu, S. Analysis of ac surface photovoltages in a depleted oxidized p-type silicon wafer. Jpn. J. Appl. Phys. 1986, 25, 807. [Google Scholar] [CrossRef]
Figure 1. Image of the silicon nanofluidic membrane. (A). Final diced membrane with a size of 6 mm × 6 mm × 0.4 mm. In the photograph, the exposed contact pads of the polysilicon electrode used for connection to the external source for the polarization potential are indicated. (B) SEM image of the highly densely packed parallel nanochannel design. (C) FIB-SEM vertical cross-section showing the membrane nanochannels. (D) FIB-SEM vertical cross-section image of the wall of a nanochannel. The layers obtained at different stages of the photolithographic processes were color-enhanced to highlight the native original p-Si of the device layer of the wafer (in gray), thermally grown silicon dioxide (SiO2, ~175 nm, in green), poly-Si deposited by LPCVD (~121 nm, in blue), and a-SiC deposited by PECVD (64 nm, in red).
Figure 1. Image of the silicon nanofluidic membrane. (A). Final diced membrane with a size of 6 mm × 6 mm × 0.4 mm. In the photograph, the exposed contact pads of the polysilicon electrode used for connection to the external source for the polarization potential are indicated. (B) SEM image of the highly densely packed parallel nanochannel design. (C) FIB-SEM vertical cross-section showing the membrane nanochannels. (D) FIB-SEM vertical cross-section image of the wall of a nanochannel. The layers obtained at different stages of the photolithographic processes were color-enhanced to highlight the native original p-Si of the device layer of the wafer (in gray), thermally grown silicon dioxide (SiO2, ~175 nm, in green), poly-Si deposited by LPCVD (~121 nm, in blue), and a-SiC deposited by PECVD (64 nm, in red).
Membranes 11 00535 g001
Figure 2. (A) Ellipsometric measurement of a-SiC blank sample. (B) a-SiC blank sample 50 μm × 50 μm 3D topographic map. (C) SiO2 blank sample 50 μm × 50 μm 3D topographic map.
Figure 2. (A) Ellipsometric measurement of a-SiC blank sample. (B) a-SiC blank sample 50 μm × 50 μm 3D topographic map. (C) SiO2 blank sample 50 μm × 50 μm 3D topographic map.
Membranes 11 00535 g002
Figure 3. (A). To perform the I-V and conductance measurements, transmembrane potential VDS is applied between the source electrode and drain electrode in the reservoir, and gate potential VGS is applied between the source electrode in the reservoir and the buried gate electrode of the membrane. (B) Telescopic illustration of the nanochannel wall and the buried gate electrode. (C). Application of gate potential VGS that polarizes the surface of the nanochannel wall, leading to alteration of the conductance of the charged species and, consequently, modulation of the ionic current IDS.
Figure 3. (A). To perform the I-V and conductance measurements, transmembrane potential VDS is applied between the source electrode and drain electrode in the reservoir, and gate potential VGS is applied between the source electrode in the reservoir and the buried gate electrode of the membrane. (B) Telescopic illustration of the nanochannel wall and the buried gate electrode. (C). Application of gate potential VGS that polarizes the surface of the nanochannel wall, leading to alteration of the conductance of the charged species and, consequently, modulation of the ionic current IDS.
Membranes 11 00535 g003
Figure 4. Measured (points) and calculated (curve) ionic conductance across the nanochannels versus the electrolyte concentration. The dashed line represents the bulk prediction, which deviates from the experimental data in the low ionic concentration region. The standard deviation of the measurements was calculated based on three membranes, tested three times each.
Figure 4. Measured (points) and calculated (curve) ionic conductance across the nanochannels versus the electrolyte concentration. The dashed line represents the bulk prediction, which deviates from the experimental data in the low ionic concentration region. The standard deviation of the measurements was calculated based on three membranes, tested three times each.
Membranes 11 00535 g004
Figure 5. Ionic current IDS when gate potential VGS and transmembrane potential VDS are applied. Red points represent Cl ions, while green points represent K+ ions. (A) VDS < 0 V and VGS < 0; (B) VDS > 0 V and VGS < 0; (C) VDS < 0 V and VGS > 0; (D) VDS > 0 V and VGS > 0.VDS < 0 V attracts positive potassium ions K+ to the drain electrode, whereas VDS > 0 V attracts negative chloride ions Cl to the source electrode. VGS > 0 V draws Cl to the nanochannel wall, creating a negatively charged EDL. Consequently, the number of negative ions in the nanochannel increases, and positive ions K+ are repelled. VGS < 0 V attracts K+ to the nanochannel walls, leading to an increased number of K+ and a decreased number of Cl.
Figure 5. Ionic current IDS when gate potential VGS and transmembrane potential VDS are applied. Red points represent Cl ions, while green points represent K+ ions. (A) VDS < 0 V and VGS < 0; (B) VDS > 0 V and VGS < 0; (C) VDS < 0 V and VGS > 0; (D) VDS > 0 V and VGS > 0.VDS < 0 V attracts positive potassium ions K+ to the drain electrode, whereas VDS > 0 V attracts negative chloride ions Cl to the source electrode. VGS > 0 V draws Cl to the nanochannel wall, creating a negatively charged EDL. Consequently, the number of negative ions in the nanochannel increases, and positive ions K+ are repelled. VGS < 0 V attracts K+ to the nanochannel walls, leading to an increased number of K+ and a decreased number of Cl.
Membranes 11 00535 g005
Figure 6. I-V (IDS vs. VDS) ionic transfer characteristics and leakage current (IGS vs. VGS) characteristics of the nanofluidic membrane measured for 10−5 M KCl ( λ ~ 96   nm ) and 10−4 M KCl ( λ ~ 30   nm ) . (A) IDS vs. VDS measured for 10−5 M KCl. (B) IGS vs. VGS measured for 10−5 M KCl. (C) IDS vs. VDS measured for 10−4 M KCl. (D) IGS vs. VGS measured for 10−4 M KCl.
Figure 6. I-V (IDS vs. VDS) ionic transfer characteristics and leakage current (IGS vs. VGS) characteristics of the nanofluidic membrane measured for 10−5 M KCl ( λ ~ 96   nm ) and 10−4 M KCl ( λ ~ 30   nm ) . (A) IDS vs. VDS measured for 10−5 M KCl. (B) IGS vs. VGS measured for 10−5 M KCl. (C) IDS vs. VDS measured for 10−4 M KCl. (D) IGS vs. VGS measured for 10−4 M KCl.
Membranes 11 00535 g006
Figure 7. Normalized conductance for 10−4 M KCl, defined as the ratio of the conductance after application of the gate voltage to the conductance measured with a floating gate for VDS = −1 V and VDS = 1 V.
Figure 7. Normalized conductance for 10−4 M KCl, defined as the ratio of the conductance after application of the gate voltage to the conductance measured with a floating gate for VDS = −1 V and VDS = 1 V.
Membranes 11 00535 g007
Figure 8. Blank setup measurements. (A) Blank sample immersed in the KCl electrolyte. (B) Band diagram of a layered structure composed of the semiconductor poly-Si, a dielectric, and an electrolyte. Accumulation for VGS < 0, flat band for VGS = 0 V, and depletion for VGS > 0 V. (C) Equivalent circuit used to fit the impedance data. RM is the electrolyte resistance, RSD and CSD are the resistance and the capacitance associated with the dielectric coating, RCT is the charge transfer resistance, and CEDL is the Helmholtz capacitance due to the EDL.
Figure 8. Blank setup measurements. (A) Blank sample immersed in the KCl electrolyte. (B) Band diagram of a layered structure composed of the semiconductor poly-Si, a dielectric, and an electrolyte. Accumulation for VGS < 0, flat band for VGS = 0 V, and depletion for VGS > 0 V. (C) Equivalent circuit used to fit the impedance data. RM is the electrolyte resistance, RSD and CSD are the resistance and the capacitance associated with the dielectric coating, RCT is the charge transfer resistance, and CEDL is the Helmholtz capacitance due to the EDL.
Membranes 11 00535 g008
Figure 9. Comparison between the fitted values for a-SiC and SiO2. (A) RCT charge transfer resistance; (B) RSD resistance of the polysilicon; (C) QEDL constant phase element and its nEDL factor associated with the EDL; (D) QSD constant phase element and its nSD factor associated with the polysilicon.
Figure 9. Comparison between the fitted values for a-SiC and SiO2. (A) RCT charge transfer resistance; (B) RSD resistance of the polysilicon; (C) QEDL constant phase element and its nEDL factor associated with the EDL; (D) QSD constant phase element and its nSD factor associated with the polysilicon.
Membranes 11 00535 g009
Figure 10. (A) Capacitance-voltage (C-V) and (B) conductance-voltage (G-V) data at a high frequency of 105 Hz evaluated on blank devices.
Figure 10. (A) Capacitance-voltage (C-V) and (B) conductance-voltage (G-V) data at a high frequency of 105 Hz evaluated on blank devices.
Membranes 11 00535 g010
Figure 11. (A) Mott–Schottky plot for a-SiC and SiO2. Flat band potential evaluation for a-SiC and SiO2 electrodes in 10−4 M KCl. (B) Extracted density of interface states for a-SiC and SiO2.
Figure 11. (A) Mott–Schottky plot for a-SiC and SiO2. Flat band potential evaluation for a-SiC and SiO2 electrodes in 10−4 M KCl. (B) Extracted density of interface states for a-SiC and SiO2.
Membranes 11 00535 g011
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Silvestri, A.; Di Trani, N.; Canavese, G.; Motto Ros, P.; Iannucci, L.; Grassini, S.; Wang, Y.; Liu, X.; Demarchi, D.; Grattoni, A. Silicon Carbide-Gated Nanofluidic Membrane for Active Control of Electrokinetic Ionic Transport. Membranes 2021, 11, 535. https://doi.org/10.3390/membranes11070535

AMA Style

Silvestri A, Di Trani N, Canavese G, Motto Ros P, Iannucci L, Grassini S, Wang Y, Liu X, Demarchi D, Grattoni A. Silicon Carbide-Gated Nanofluidic Membrane for Active Control of Electrokinetic Ionic Transport. Membranes. 2021; 11(7):535. https://doi.org/10.3390/membranes11070535

Chicago/Turabian Style

Silvestri, Antonia, Nicola Di Trani, Giancarlo Canavese, Paolo Motto Ros, Leonardo Iannucci, Sabrina Grassini, Yu Wang, Xuewu Liu, Danilo Demarchi, and Alessandro Grattoni. 2021. "Silicon Carbide-Gated Nanofluidic Membrane for Active Control of Electrokinetic Ionic Transport" Membranes 11, no. 7: 535. https://doi.org/10.3390/membranes11070535

APA Style

Silvestri, A., Di Trani, N., Canavese, G., Motto Ros, P., Iannucci, L., Grassini, S., Wang, Y., Liu, X., Demarchi, D., & Grattoni, A. (2021). Silicon Carbide-Gated Nanofluidic Membrane for Active Control of Electrokinetic Ionic Transport. Membranes, 11(7), 535. https://doi.org/10.3390/membranes11070535

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop