Mesenchymal Stem Cells Do Not Lose Direct Labels Including Iron Oxide Nanoparticles and DFO-89Zr Chelates through Secretion of Extracellular Vesicles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Magnetic Labeling of HMSCs
2.3. EV Isolation
2.4. Tunable Resistive Pulse Sensing (TRPS)
2.5. Flow Cytometry
2.6. In Vivo MR Imaging of EVs Derived from Molday ION-Labeled HMSCs
2.7. 89Zr Labelling of Cells for EVs Production
2.8. Image Processing and Statistical Analysis
3. Results
3.1. HMSCs Labeling with Molday ION
3.2. The Effect of Molday ION Treatment on the Number and Size of HMSCs-Derived EVs
3.3. The Effect of Molday ION Treatment on the Zeta Potential of HMSCs-Derived EVs
3.4. Characterization of HMSCs-Derived EVs and the Efficacy of Labeling EVs with Molday ION
3.5. In Vivo MR Imaging of EVs Derived from Molday ION-Treated HMSCs
3.6. The Passing of 89Zr-DFO-Based Radiolabel from MSCs to EVs Derived from Them
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Gao, Y.; Jablonska, A.; Chu, C.; Walczak, P.; Janowski, M. Mesenchymal Stem Cells Do Not Lose Direct Labels Including Iron Oxide Nanoparticles and DFO-89Zr Chelates through Secretion of Extracellular Vesicles. Membranes 2021, 11, 484. https://doi.org/10.3390/membranes11070484
Gao Y, Jablonska A, Chu C, Walczak P, Janowski M. Mesenchymal Stem Cells Do Not Lose Direct Labels Including Iron Oxide Nanoparticles and DFO-89Zr Chelates through Secretion of Extracellular Vesicles. Membranes. 2021; 11(7):484. https://doi.org/10.3390/membranes11070484
Chicago/Turabian StyleGao, Yue, Anna Jablonska, Chengyan Chu, Piotr Walczak, and Miroslaw Janowski. 2021. "Mesenchymal Stem Cells Do Not Lose Direct Labels Including Iron Oxide Nanoparticles and DFO-89Zr Chelates through Secretion of Extracellular Vesicles" Membranes 11, no. 7: 484. https://doi.org/10.3390/membranes11070484
APA StyleGao, Y., Jablonska, A., Chu, C., Walczak, P., & Janowski, M. (2021). Mesenchymal Stem Cells Do Not Lose Direct Labels Including Iron Oxide Nanoparticles and DFO-89Zr Chelates through Secretion of Extracellular Vesicles. Membranes, 11(7), 484. https://doi.org/10.3390/membranes11070484