Mitochondria Isolated from Hearts Subjected to Ischemia/Reperfusion Benefit from Adenine Nucleotide Translocase 1 Overexpression
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Langendorff Perfusion (Volume-Constant Mode)
2.3. Infarct Size Measurement
2.4. Real-Time RT-PCR Analysis
2.5. Western Blot Analysis
2.6. Mitochondria Isolation
2.7. Calcium Retention Assay
2.8. Membrane Potential (Δψm)
2.9. Oxygen Consumption
2.10. Statistical Analysis
3. Results
3.1. ANT Expression and Modification in I/R-Treated Hearts
3.2. ANT1 Overexpression Stabilizes Mitochondrial Calcium Retention Capacity and Membrane Potential after I/R
3.3. ANT1 Overexpression Stabilizes Mitochondrial Oxygen Consumption after I/R
3.4. ANT1 Overexpression Reduces the PLB/SERCA2a Ratio and Stabilizes a Beneficial pPLB/PLB Ratio Leading to Reduced Diastolic Pressure after I/R
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Dörner, A.; Lynetskiy, O.; Euler, G.; Landmesser, U.; Schlüter, K.-D.; Heger, J. Mitochondria Isolated from Hearts Subjected to Ischemia/Reperfusion Benefit from Adenine Nucleotide Translocase 1 Overexpression. Membranes 2021, 11, 836. https://doi.org/10.3390/membranes11110836
Dörner A, Lynetskiy O, Euler G, Landmesser U, Schlüter K-D, Heger J. Mitochondria Isolated from Hearts Subjected to Ischemia/Reperfusion Benefit from Adenine Nucleotide Translocase 1 Overexpression. Membranes. 2021; 11(11):836. https://doi.org/10.3390/membranes11110836
Chicago/Turabian StyleDörner, Andrea, Oleg Lynetskiy, Gerhild Euler, Ulf Landmesser, Klaus-Dieter Schlüter, and Jacqueline Heger. 2021. "Mitochondria Isolated from Hearts Subjected to Ischemia/Reperfusion Benefit from Adenine Nucleotide Translocase 1 Overexpression" Membranes 11, no. 11: 836. https://doi.org/10.3390/membranes11110836