Next Article in Journal
Finding Children with High Risk of Non-Vaccination in 92 Low- and Middle-Income Countries: A Decision Tree Approach
Next Article in Special Issue
What Constitutes Protective Immunity Following Yellow Fever Vaccination?
Previous Article in Journal
Attitude towards Vaccination among Health Science Students before the COVID-19 Pandemic
Previous Article in Special Issue
Prophylactic Multi-Subunit Vaccine against Chlamydia trachomatis: In Vivo Evaluation in Mice
Article

Structure and Immunogenicity of the Bordetella pertussis LOS-Derived Oligosaccharides in the Endosomal-Like Pre-Processing Mice Model

Hirszfeld Institute of Immunology and Experimental Therapy, 53-114 Wroclaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Vasso Apostolopoulos
Vaccines 2021, 9(6), 645; https://doi.org/10.3390/vaccines9060645
Received: 12 May 2021 / Revised: 28 May 2021 / Accepted: 8 June 2021 / Published: 13 June 2021
(This article belongs to the Special Issue Vaccines for Infectious and Chronic Diseases)
Glycoproteins are processed endosomally prior to presentation to T cells and subsequent induction of specific antibodies. The sugar part of glycoconjugate may be degraded while the type of the process depends on the features of the particular structure. The generated carbohydrate epitopes may differ from native structures and influence immunogenicity of the antigens. We have devised a model of endosomal-like pre-processing of Bordetella pertussis 186 oligosaccharides (OSs) to verify how it affects the immunogenicity of their conjugates. The glycoconjugates of structurally defined forms of the dodecasaccharide OS were synthesized and their immunogenicity was assessed using immunochemical methods. The structural features of the oligosaccharides and their sensitivity to deamination were analyzed by NMR spectroscopy. The distal trisaccharide-comprising pentasaccharide conjugated to a protein was the most effective in inducing immune response against the B. pertussis 186 LOS and the immune response to the complete OS conjugates was significantly lower. This could be explained by the loss of the distal trisaccharide during the in-cell deamination process suggesting that the native structure is not optimal for a vaccine antigen. Consequently, our research has shown that designing of new glycoconjugate vaccines requires the antigen structures to be verified in context of possible endosomal reactions beforehand. View Full-Text
Keywords: Bordetella pertussis; lipooligosaccharide; core oligosaccharide; neoglycoconjugates; endosomal glycan processing; reactive nitrogen species; vaccine design Bordetella pertussis; lipooligosaccharide; core oligosaccharide; neoglycoconjugates; endosomal glycan processing; reactive nitrogen species; vaccine design
Show Figures

Graphical abstract

MDPI and ACS Style

Koj, S.; Ucieklak, K.; Lugowski, C.; Niedziela, T. Structure and Immunogenicity of the Bordetella pertussis LOS-Derived Oligosaccharides in the Endosomal-Like Pre-Processing Mice Model. Vaccines 2021, 9, 645. https://doi.org/10.3390/vaccines9060645

AMA Style

Koj S, Ucieklak K, Lugowski C, Niedziela T. Structure and Immunogenicity of the Bordetella pertussis LOS-Derived Oligosaccharides in the Endosomal-Like Pre-Processing Mice Model. Vaccines. 2021; 9(6):645. https://doi.org/10.3390/vaccines9060645

Chicago/Turabian Style

Koj, Sabina, Karolina Ucieklak, Czeslaw Lugowski, and Tomasz Niedziela. 2021. "Structure and Immunogenicity of the Bordetella pertussis LOS-Derived Oligosaccharides in the Endosomal-Like Pre-Processing Mice Model" Vaccines 9, no. 6: 645. https://doi.org/10.3390/vaccines9060645

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop