African Trypanosomosis Obliterates DTPa Vaccine-Induced Functional Memory So That Post-Treatment Bordetella pertussis Challenge Fails to Trigger a Protective Recall Response
Abstract
1. Introduction
2. Materials and Methods
2.1. Mice and Parasites Infections
2.2. DTPa Vaccine Procedure and In Vivo B. pertussis Challenge
2.3. Quantification of Cytokines by ELISA
2.4. Quantification of Anti-Pertussis and Anti-VSG Antibody Titers by ELISA
2.5. Cell Preparation and Flow Cytometry Analysis
2.6. Flow Cytometry Detection Reagents
2.7. Statistical Analysis
3. Results
3.1. T. b. brucei Destroys DTPa Vaccine-Induced Protection against Bordetella Pertussis
3.2. T. b. brucei Infection Alters the IgG1/IgG2a Ratio of Anti-Pertussis Antibody Titers in DTPa-Vaccinated Mice
3.3. T. b. brucei Infection Gives Rise to the Rapid Destruction of the Host Spleen B Cell Compartment, While Anti-Trypanosome Treatment Results in a Full Cellular Spleen Recovery
3.4. DTPa Vaccination Results in the Temporary Improvement of Trypanosomosis Control Coinciding with an Increased Anti-Parasite IFNγ Response
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Radwanska, M.; Nguyen, H.T.T.; Magez, S. African Trypanosomosis Obliterates DTPa Vaccine-Induced Functional Memory So That Post-Treatment Bordetella pertussis Challenge Fails to Trigger a Protective Recall Response. Vaccines 2021, 9, 603. https://doi.org/10.3390/vaccines9060603
Radwanska M, Nguyen HTT, Magez S. African Trypanosomosis Obliterates DTPa Vaccine-Induced Functional Memory So That Post-Treatment Bordetella pertussis Challenge Fails to Trigger a Protective Recall Response. Vaccines. 2021; 9(6):603. https://doi.org/10.3390/vaccines9060603
Chicago/Turabian StyleRadwanska, Magdalena, Hang Thi Thu Nguyen, and Stefan Magez. 2021. "African Trypanosomosis Obliterates DTPa Vaccine-Induced Functional Memory So That Post-Treatment Bordetella pertussis Challenge Fails to Trigger a Protective Recall Response" Vaccines 9, no. 6: 603. https://doi.org/10.3390/vaccines9060603
APA StyleRadwanska, M., Nguyen, H. T. T., & Magez, S. (2021). African Trypanosomosis Obliterates DTPa Vaccine-Induced Functional Memory So That Post-Treatment Bordetella pertussis Challenge Fails to Trigger a Protective Recall Response. Vaccines, 9(6), 603. https://doi.org/10.3390/vaccines9060603