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Article

Priming with Recombinant BCG Expressing HTI Enhances the Magnitude and Breadth of the T-Cell Immune Responses Elicited by MVA.HTI in BALB/c Mice

1
Vall d’Hebron Research Institute, 08035 Barcelona, Spain
2
EAVI2020 European AIDS Vaccine Initiative H2020 Research Programme, London SW7 2BU, UK
3
Irsicaixa AIDS Research Institute, 08916 Badalona, Spain
4
Biosciences Department, Universitat de Vic-Universitat Central de Catalunya (UVic-UCC), 08500 Vic, Barcelona, Spain
5
The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford OX1 2JD, UK
6
International Research Center of Medical Sciences (IRCMS), Kumamoto University, Kumamoto 860-8555, Japan
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ICREA, Pg. Lluís Companys 23, 08010 Barcelona, Spain
8
AELIX Therapeutics, 08028 Barcelona, Spain
9
Microbiology Department, Vall d’Hebron University Hospital, 08035 Barcelona, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Vaccines 2020, 8(4), 678; https://doi.org/10.3390/vaccines8040678
Received: 16 October 2020 / Revised: 9 November 2020 / Accepted: 11 November 2020 / Published: 13 November 2020
(This article belongs to the Special Issue B and T Cell-Mediated Immunity)
The use of Mycobacterium bovis bacillus Calmette–Guérin (BCG) as a live vaccine vehicle is a promising approach for HIV-1-specific T-cell induction. In this study, we used recombinant BCG expressing HIVACAT T-cell immunogen (HTI), BCG.HTI2auxo.int. BALB/c mice immunization with BCG.HTI2auxo.int prime and MVA.HTI boost was safe and induced HIV-1-specific T-cell responses. Two weeks after boost, T-cell responses were assessed by IFN-γ ELISpot. The highest total magnitude of IFN-γ spot-forming cells (SFC)/106 splenocytes was observed in BCG.HTI2auxo.int primed mice compared to mice receiving MVA.HTI alone or mice primed with BCGwt, although the differences between the vaccination regimens only reached trends. In order to evaluate the differences in the breadth of the T-cell immune responses, we examined the number of reactive peptide pools per mouse. Interestingly, both BCG.HTI2auxo.int and BCGwt primed mice recognized an average of four peptide pools per mouse. However, the variation was higher in BCG.HTI2auxo.int primed mice with one mouse recognizing 11 peptide pools and three mice recognizing few or no peptide pools. The recognition profile appeared to be more spread out for BCG.HTI2auxo.int primed mice and mice only receiving MVA.HTI. Here, we describe a useful vaccine platform for priming protective responses against HIV-1/TB and other prevalent infectious diseases. View Full-Text
Keywords: BCG; HIV-1; vaccine; rBCG; HTI; T-cell BCG; HIV-1; vaccine; rBCG; HTI; T-cell
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MDPI and ACS Style

Saubi, N.; Kilpeläinen, A.; Eto, Y.; Chen, C.-W.; Olvera, À.; Hanke, T.; Brander, C.; Joseph-Munné, J. Priming with Recombinant BCG Expressing HTI Enhances the Magnitude and Breadth of the T-Cell Immune Responses Elicited by MVA.HTI in BALB/c Mice. Vaccines 2020, 8, 678. https://doi.org/10.3390/vaccines8040678

AMA Style

Saubi N, Kilpeläinen A, Eto Y, Chen C-W, Olvera À, Hanke T, Brander C, Joseph-Munné J. Priming with Recombinant BCG Expressing HTI Enhances the Magnitude and Breadth of the T-Cell Immune Responses Elicited by MVA.HTI in BALB/c Mice. Vaccines. 2020; 8(4):678. https://doi.org/10.3390/vaccines8040678

Chicago/Turabian Style

Saubi, Narcís, Athina Kilpeläinen, Yoshiki Eto, Chun-Wei Chen, Àlex Olvera, Tomáš Hanke, Christian Brander, and Joan Joseph-Munné. 2020. "Priming with Recombinant BCG Expressing HTI Enhances the Magnitude and Breadth of the T-Cell Immune Responses Elicited by MVA.HTI in BALB/c Mice" Vaccines 8, no. 4: 678. https://doi.org/10.3390/vaccines8040678

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