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Article

Association of Porcine Swine Leukocyte Antigen (SLA) Haplotypes with B- and T-Cell Immune Response to Foot-and-Mouth Disease Virus (FMDV) Peptides

1
Centro de Biología Molecular “Severo Ochoa” (CSIC-UAM), 28049 Madrid, Spain
2
Computer Science Department, Universidad Carlos III of Madrid, Leganés, 28911 Madrid, Spain
3
Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, 08003 Barcelona, Spain
4
Computer Science Department, Universidad Rey Juan Carlos, 28933 Móstoles, Spain
5
Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid, Spain
6
Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, A-1210 Vienna, Austria
*
Authors to whom correspondence should be addressed.
The authors contributed equally to this manuscript.
Vaccines 2020, 8(3), 513; https://doi.org/10.3390/vaccines8030513
Received: 25 June 2020 / Revised: 15 August 2020 / Accepted: 1 September 2020 / Published: 8 September 2020
(This article belongs to the Special Issue Evaluation of Vaccine Immunogenicity)
Dendrimer peptides are promising vaccine candidates against the foot-and-mouth disease virus (FMDV). Several B-cell epitope (B2T) dendrimers, harboring a major FMDV antigenic B-cell site in VP1 protein, are covalently linked to heterotypic T-cell epitopes from 3A and/or 3D proteins, and elicited consistent levels of neutralizing antibodies and IFN-γ-producing cells in pigs. To address the contribution of the highly polymorphic nature of the porcine MHC (SLA, swine leukocyte antigen) on the immunogenicity of B2T dendrimers, low-resolution (Lr) haplotyping was performed. We looked for possible correlations between particular Lr haplotypes with neutralizing antibody and T-cell responses induced by B2T peptides. In this study, 63 pigs immunized with B2T dendrimers and 10 non-immunized (control) animals are analyzed. The results reveal a robust significant correlation between SLA class-II Lr haplotypes and the T-cell response. Similar correlations of T-cell response with SLA class-I Lr haplotypes, and between B-cell antibody response and SLA class-I and SLA class-II Lr haplotypes, were only found when the sample was reduced to animals with Lr haplotypes represented more than once. These results support the contribution of SLA class-II restricted T-cells to the magnitude of the T-cell response and to the antibody response evoked by the B2T dendrimers, being of potential value for peptide vaccine design against FMDV. View Full-Text
Keywords: swine; foot-and-mouth disease virus; dendrimer peptide; swine leukocyte antigen; SLA polymorphism; adaptive immunity; vaccine; protection swine; foot-and-mouth disease virus; dendrimer peptide; swine leukocyte antigen; SLA polymorphism; adaptive immunity; vaccine; protection
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MDPI and ACS Style

de León, P.; Cañas-Arranz, R.; Saez, Y.; Forner, M.; Defaus, S.; Cuadra, D.; Bustos, M.J.; Torres, E.; Andreu, D.; Blanco, E.; Sobrino, F.; Hammer, S.E. Association of Porcine Swine Leukocyte Antigen (SLA) Haplotypes with B- and T-Cell Immune Response to Foot-and-Mouth Disease Virus (FMDV) Peptides. Vaccines 2020, 8, 513. https://doi.org/10.3390/vaccines8030513

AMA Style

de León P, Cañas-Arranz R, Saez Y, Forner M, Defaus S, Cuadra D, Bustos MJ, Torres E, Andreu D, Blanco E, Sobrino F, Hammer SE. Association of Porcine Swine Leukocyte Antigen (SLA) Haplotypes with B- and T-Cell Immune Response to Foot-and-Mouth Disease Virus (FMDV) Peptides. Vaccines. 2020; 8(3):513. https://doi.org/10.3390/vaccines8030513

Chicago/Turabian Style

de León, Patricia, Rodrigo Cañas-Arranz, Yago Saez, Mar Forner, Sira Defaus, Dolores Cuadra, María J. Bustos, Elisa Torres, David Andreu, Esther Blanco, Francisco Sobrino, and Sabine E. Hammer 2020. "Association of Porcine Swine Leukocyte Antigen (SLA) Haplotypes with B- and T-Cell Immune Response to Foot-and-Mouth Disease Virus (FMDV) Peptides" Vaccines 8, no. 3: 513. https://doi.org/10.3390/vaccines8030513

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