LvYY1 Activates WSSV ie1 Promoter for Enhanced Vaccine Production and Efficacy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Viruses and Cells
2.2. Construction of Baculoviral Expression Vectors Containing LvYY1
2.3. Generation and Titration of Recombinant Baculovirus
2.4. Immunofluorescence Assay (IFA)
2.5. Western Blot
2.6. Flow Cytometry
2.7. Gel Shift Assays
2.8. Hemagglutination Assays
2.9. Transduction and Quantitative PCR (qPCR)
2.10. Animal Study
2.11. Serological Assays
2.12. Hemagglutination Inhibition (HI)
2.13. Statistical Analysis
2.14. Ethical Approval and Informed Consent
3. Results
3.1. LvYY1 Expression and Interaction with WSSV ie1 Promoter in Baculovirus
3.2. LvYY1 Enhanced eGFP Expression Driven by WSSV ie1 Promoter
3.3. LvYY1 Improved CSFV E2 Secretion under WSSV ie1 Promoter in Sf-9 Cells
3.4. LvYY1 Stimulated Active and Functional H5 Hemagglutinin Expression in Baculovirus
3.5. LvYY1-ie1 Baculovirus Mediated H5 Transduction in CEF Cells
3.6. LvYY1-ie1 Baculovirus Presented Improved Protective Immunogenicity in Chicken against H5 AIVs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Primer | Oligonucleotides (from 5′to 3′) | Product Size/bp |
---|---|---|
YY1-Flag-F | ATGGCCTCCTCCGACTTCGTGACCG | 1089 |
YY1-Flag-R | TTACTTATCGTCGTCATCCTTGTAATCGGAGGAGTAGATCACGAATT | |
ie1-F | ACGCGTTTGGCGTTTTATTTTCTTG | 518 |
ie1-R | GGGCTTGAGTGGAGAGAGAGAGCTA | |
eGFP-F | CTCTCTCTCCACTCAAGCCCATGGTGAGCAAGGGCGAGGAG | 720 |
eGFP-R | TTACTTGTACAGCTCGTCCATGCCGAGA | |
E2-F | ATGATAAAAGTATTAAGAGGGCAGG | 1068 |
E2-R | CTACACGTCCAGGTCAAACCAGTAT | |
HA-F | ATGGAGAAAATAGTGCTTCTTCTTGC | 1704 |
HA-R | TTAAATGCAAATTCTGCATTGTAAC | |
HA-F1 | GTCAAGAAAGGGGACTCAGCA | 181 |
HA-R2 | GCCCAGTCGCAAGGACTAAT | |
β-actin-F | GAG AAATTGTGCGTGACATCA | 152 |
β-actin-R | CCTGAACCTCTCATTGCCA |
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Tao, L.-N.; Liu, Z.-H.; Xu, H.-L.; Lu, Y.; Liao, M.; He, F. LvYY1 Activates WSSV ie1 Promoter for Enhanced Vaccine Production and Efficacy. Vaccines 2020, 8, 510. https://doi.org/10.3390/vaccines8030510
Tao L-N, Liu Z-H, Xu H-L, Lu Y, Liao M, He F. LvYY1 Activates WSSV ie1 Promoter for Enhanced Vaccine Production and Efficacy. Vaccines. 2020; 8(3):510. https://doi.org/10.3390/vaccines8030510
Chicago/Turabian StyleTao, Li-Na, Ze-Hui Liu, Hui-Ling Xu, Ying Lu, Min Liao, and Fang He. 2020. "LvYY1 Activates WSSV ie1 Promoter for Enhanced Vaccine Production and Efficacy" Vaccines 8, no. 3: 510. https://doi.org/10.3390/vaccines8030510