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Open AccessArticle

Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure

1
The Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, 500 Irvin Court Suite 200, Decatur, GA 30030, USA
2
Department of Obstetrics and Gynecology, University of Kansas Medical Center, 3901 Rainbow Blvd, Kansas City, KS 66160, USA
3
Department of Pediatrics, Emory University School of Medicine, 2015 Uppergate Drive NE, Atlanta, GA 30322, USA
4
The EMMES Company, LLC, 401 N. Washington St., Suite 700, Rockville, MD 20850, USA
5
Cota Enterprises, 16570 46th Street, McLouth, KS 66054, USA
6
Division of Microbiology and Infectious Diseases, NIAID, NIH, 5601 Fishers Lane, Rockville, MD 20892-9825, USA
7
Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA 30329, USA
8
New York University Langone Vaccine Center, Alexandria Center for Life Sciences (West Tower), 430 E 29th St, Room 304, New York, NY 10016, USA
*
Author to whom correspondence should be addressed.
The first two authors contributed equally to this research project.
Vaccines 2020, 8(3), 348; https://doi.org/10.3390/vaccines8030348
Received: 1 June 2020 / Revised: 22 June 2020 / Accepted: 24 June 2020 / Published: 30 June 2020
Human papillomavirus virus (HPV) vaccines aim to provide durable protection and are ideal to study the association of cellular with humoral responses. We assessed the duration and characteristics of immune responses provided by the quadrivalent HPV (4vHPV) vaccine in healthy female adults with or without prior exposure with type 16 and 18 HPV. In a prospective cohort, vaccine naïve females received three doses of 4vHPV vaccine and were followed for two years to assess cellular (intracellular cytokine staining, proliferation and B cell ELISpot assays) and humoral (multiplex L1/L2 viral-like particles (VLP) and M4 ELISAs) responses. Frequencies of vaccine-specific CD4+ T cells correlated with antibody responses. Higher HPV antibody titers were found at all time points in participants previously exposed to HPV, except for anti-HPV-18 at Day 187 (one week post the third vaccination). Retrospective cohorts enrolled females who had previously received two or three 4vHPV doses and tested antibody titers by M4 ELISA and pseudovirion neutralization assay along with memory B cells (MBCs). Almost all women enrolled in a retrospective cohort with two prior doses and all women enrolled in a retrospective cohort with three prior doses had sustained antibody and memory responses. Our findings indicate that HPV vaccination induces a long-lasting, robust cellular and humoral immune responses. View Full-Text
Keywords: HPV vaccine; memory B cell responses; T cell responses; ELISA; pseudovirion neutralization assay HPV vaccine; memory B cell responses; T cell responses; ELISA; pseudovirion neutralization assay
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Lai, L.; Ault, K.; Rouphael, N.; Beck, A.; Domjahn, B.; Xu, Y.; Anderson, E.J.; Cheng, A.; Nakamura, A.; Hoagland, R.J.; Kelley, C.; Edupuganti, S.; Mask, K.; Nesin, M.; Unger, E.R.; Panicker, G.; David, H.; Mulligan, M.J. Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure. Vaccines 2020, 8, 348.

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