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Open AccessArticle

N-terminus of Flagellin Fused to an Antigen Improves Vaccine Efficacy against Pasteurella Multocida Infection in Chickens

1
International Degree Program of Animal Vaccine Technology, International College, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan
2
Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(2), 283; https://doi.org/10.3390/vaccines8020283
Received: 7 May 2020 / Revised: 2 June 2020 / Accepted: 4 June 2020 / Published: 6 June 2020
(This article belongs to the Section Vaccine Adjuvants)
Flagellin from bacteria elicits a proinflammatory immune response and may act as a vaccine adjuvant. In this study, we evaluated the adjuvant effect of the N-terminus of flagellin (residues 1–99) when linked to an antigen (a truncated, conserved domain of lipoprotein E of Pasteurella multocida). Immunization of chickens with the antigen-adjuvant chimeric protein showed that the N-terminus of flagellin accelerated the antibody response and enhanced the cellular immunity (CD8+ T cell expansion). Stimulation of peripheral blood mononuclear cells from vaccinated chickens showed both TH1 (IFN-γ and IL-12) and TH2 (IL-4)-type cytokine gene expressions. In a challenge test, the N-terminus of flagellin increased the survival rate to 75%, compared to 25% in the antigen-only group. In conclusion, our study found that the N-terminus of flagellin can increase the immune response and enhance vaccine protection. View Full-Text
Keywords: flagellin; adjuvant; N-terminus; chimeric protein; Pasteurella multocida; lipoprotein E flagellin; adjuvant; N-terminus; chimeric protein; Pasteurella multocida; lipoprotein E
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Doan, T.-D.; Wang, H.-Y.; Ke, G.-M.; Cheng, L.-T. N-terminus of Flagellin Fused to an Antigen Improves Vaccine Efficacy against Pasteurella Multocida Infection in Chickens. Vaccines 2020, 8, 283.

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