Addition of Partial Envelope Domain II into Envelope Domain III of Dengue Virus Antigen Potentiates the Induction of Virus-Neutralizing Antibodies and Induces Protective Immunity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Materials and Animals
2.2. Cell Culture
2.3. Production of Recombinant Ags
2.4. Immunization and Ab Purification
2.5. Virus Propagation, Titration, and Neutralization
2.6. Ab Binding Assay
2.7. Neutralization Assay Using U937-DC-SIGN Cells
2.8. DENV Challenge Infection in AG129 Mice
2.9. Blood Cell Isolation and Serum Cytokine Analysis
2.10. Reverse-Transcription Quantitative PCR (RT-qPCR)
2.11. ADE Assay Using U937 Cells
2.12. Statistical Analysis
3. Results
3.1. EDII of DENV4 Enhanced the Induction of Abs Capable of Binding to DENV4 and Neutralizing DENV3 and DENV4
3.2. Anti-EDII-cEDIII Abs Neutralized the Four DENV Serotypes in U937-DC-SIGN Cells
3.3. ADE Was Triggered by Anti-EDII-cEDIII and Anti-cEDIII Abs
3.4. Anti-EDII-cEDIII Ab Increased the Survival of DENV-Infected Mice
3.5. Anti-EDII-cEDIII Ab Reduced Initial DENV Levels in Axillary Lymph Nodes and Spleen
3.6. Anti-EDII-cEDIII Ab Inhibited DENV Infection of Lymphocytes
3.7. Anti-EDII-cEDIII Ab Inhibited Secondary Infection of the Liver and Small Intestine
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Park, J.; Lee, H.-Y.; Khai, L.T.; Thuy, N.T.T.; Mai, L.Q.; Jang, Y.-S. Addition of Partial Envelope Domain II into Envelope Domain III of Dengue Virus Antigen Potentiates the Induction of Virus-Neutralizing Antibodies and Induces Protective Immunity. Vaccines 2020, 8, 88. https://doi.org/10.3390/vaccines8010088
Park J, Lee H-Y, Khai LT, Thuy NTT, Mai LQ, Jang Y-S. Addition of Partial Envelope Domain II into Envelope Domain III of Dengue Virus Antigen Potentiates the Induction of Virus-Neutralizing Antibodies and Induces Protective Immunity. Vaccines. 2020; 8(1):88. https://doi.org/10.3390/vaccines8010088
Chicago/Turabian StylePark, Jisang, Hyun-Young Lee, Ly Tuan Khai, Nguyen Thi Thu Thuy, Le Quynh Mai, and Yong-Suk Jang. 2020. "Addition of Partial Envelope Domain II into Envelope Domain III of Dengue Virus Antigen Potentiates the Induction of Virus-Neutralizing Antibodies and Induces Protective Immunity" Vaccines 8, no. 1: 88. https://doi.org/10.3390/vaccines8010088