Tick-Borne Encephalitis Virus Vaccines Contain Non-Structural Protein 1 Antigen and May Elicit NS1-Specific Antibody Responses in Vaccinated Individuals
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Virus
2.3. Vaccines
2.4. Mass Spectrometry
2.5. Patient Samples
2.6. Enzyme-Linked Immunosorbent Assay (ELISA)
2.7. Western Blot
2.8. Immunization of Mice with TBEV Vaccines
2.9. NS1 Immunization in Mice
2.10. Statistical Analysis
3. Results
3.1. Mass Spectrometry Analysis
3.2. NS1-Specific Antibody Response in the Immunized Mice
3.3. Detection of NS1-Specific Antibodies in Sera From TBE Patients and Vaccinees
3.4. NS1 Immunization in Mice
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Salat, J.; Mikulasek, K.; Larralde, O.; Pokorna Formanova, P.; Chrdle, A.; Haviernik, J.; Elsterova, J.; Teislerova, D.; Palus, M.; Eyer, L.; et al. Tick-Borne Encephalitis Virus Vaccines Contain Non-Structural Protein 1 Antigen and May Elicit NS1-Specific Antibody Responses in Vaccinated Individuals. Vaccines 2020, 8, 81. https://doi.org/10.3390/vaccines8010081
Salat J, Mikulasek K, Larralde O, Pokorna Formanova P, Chrdle A, Haviernik J, Elsterova J, Teislerova D, Palus M, Eyer L, et al. Tick-Borne Encephalitis Virus Vaccines Contain Non-Structural Protein 1 Antigen and May Elicit NS1-Specific Antibody Responses in Vaccinated Individuals. Vaccines. 2020; 8(1):81. https://doi.org/10.3390/vaccines8010081
Chicago/Turabian StyleSalat, Jiri, Kamil Mikulasek, Osmany Larralde, Petra Pokorna Formanova, Ales Chrdle, Jan Haviernik, Jana Elsterova, Dana Teislerova, Martin Palus, Ludek Eyer, and et al. 2020. "Tick-Borne Encephalitis Virus Vaccines Contain Non-Structural Protein 1 Antigen and May Elicit NS1-Specific Antibody Responses in Vaccinated Individuals" Vaccines 8, no. 1: 81. https://doi.org/10.3390/vaccines8010081
APA StyleSalat, J., Mikulasek, K., Larralde, O., Pokorna Formanova, P., Chrdle, A., Haviernik, J., Elsterova, J., Teislerova, D., Palus, M., Eyer, L., Zdrahal, Z., Petrik, J., & Ruzek, D. (2020). Tick-Borne Encephalitis Virus Vaccines Contain Non-Structural Protein 1 Antigen and May Elicit NS1-Specific Antibody Responses in Vaccinated Individuals. Vaccines, 8(1), 81. https://doi.org/10.3390/vaccines8010081