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Effective Activation of Human Antigen-Presenting Cells and Cytotoxic CD8+ T Cells by a Calcium Phosphate-Based Nanoparticle Vaccine Delivery System

1
Institute of Medical Microbiology, University of Duisburg-Essen, 45122 Essen, Germany
2
Institute for Virology, University of Duisburg-Essen, 45122 Essen, Germany
3
Institute for Experimental Immunology and Imaging, University of Duisburg-Essen, 45122 Essen, Germany
4
Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, 45122 Essen, Germany
5
Institute of Virology, Heinrich-Heine-University, Medical Faculty, 40225 Düsseldorf, Germany
*
Author to whom correspondence should be addressed.
Authors share first authorship.
Vaccines 2020, 8(1), 110; https://doi.org/10.3390/vaccines8010110
Received: 15 January 2020 / Revised: 12 February 2020 / Accepted: 20 February 2020 / Published: 1 March 2020
(This article belongs to the Special Issue Nanotechnology in Vaccine)
The ability of vaccines to induce T cell responses is crucial for preventing diseases caused by viruses. Nanoparticles (NPs) are considered to be efficient tools for the initiation of potent immune responses. Calcium phosphate (CaP) NPs are a class of biodegradable nanocarriers that are able to deliver immune activating molecules across physiological barriers. Therefore, the aim of this study was to assess whether Toll-like receptor (TLR) ligand and viral antigen functionalized CaP NPs are capable of inducing efficient maturation of human antigen presenting cells (APC). To achieve this, we generated primary human dendritic cells (DCs) and stimulated them with CpG or poly(I:C) functionalized CaP NPs. DCs were profoundly stronger when activated upon NP stimulation compared to treatment with soluble TLR ligands. This is indicated by increased levels of costimulatory molecules and the secretion of proinflammatory cytokines. Consequently, coculture of NP-stimulated APCs with CD8+ T cells resulted in a significant expansion of virus-specific T cells. In summary, our data suggest that functionalized CaP NPs are a suitable tool for activating human virus-specific CD8+ T cells and may represent an excellent vaccine delivery system. View Full-Text
Keywords: nanoparticles; vaccine; cytotoxic T cells; immune modulation; immunotherapy nanoparticles; vaccine; cytotoxic T cells; immune modulation; immunotherapy
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MDPI and ACS Style

Scheffel, F.; Knuschke, T.; Otto, L.; Kollenda, S.; Sokolova, V.; Cosmovici, C.; Buer, J.; Timm, J.; Epple, M.; Westendorf, A.M. Effective Activation of Human Antigen-Presenting Cells and Cytotoxic CD8+ T Cells by a Calcium Phosphate-Based Nanoparticle Vaccine Delivery System. Vaccines 2020, 8, 110. https://doi.org/10.3390/vaccines8010110

AMA Style

Scheffel F, Knuschke T, Otto L, Kollenda S, Sokolova V, Cosmovici C, Buer J, Timm J, Epple M, Westendorf AM. Effective Activation of Human Antigen-Presenting Cells and Cytotoxic CD8+ T Cells by a Calcium Phosphate-Based Nanoparticle Vaccine Delivery System. Vaccines. 2020; 8(1):110. https://doi.org/10.3390/vaccines8010110

Chicago/Turabian Style

Scheffel, Florian, Torben Knuschke, Lucas Otto, Sebastian Kollenda, Viktoriya Sokolova, Christine Cosmovici, Jan Buer, Jörg Timm, Matthias Epple, and Astrid M. Westendorf 2020. "Effective Activation of Human Antigen-Presenting Cells and Cytotoxic CD8+ T Cells by a Calcium Phosphate-Based Nanoparticle Vaccine Delivery System" Vaccines 8, no. 1: 110. https://doi.org/10.3390/vaccines8010110

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